1H, 13C, and 15N Chemical Shift Assignments for Tn916 N-terminal DNA Binding Domain complex with DNA
Polymer type: polypeptide(L) polydeoxyribonucleotide
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 64.2 % (738 of 1150) | 56.8 % (415 of 730) | 72.6 % (249 of 343) | 96.1 % (74 of 77) |
Backbone | 57.4 % (357 of 622) | 43.1 % (144 of 334) | 66.7 % (144 of 216) | 95.8 % (69 of 72) |
Sidechain | 74.5 % (444 of 596) | 68.4 % (271 of 396) | 86.2 % (168 of 195) | 100.0 % (5 of 5) |
Aromatic | 31.7 % (38 of 120) | 22.1 % (19 of 86) | 54.5 % (18 of 33) | 100.0 % (1 of 1) |
Methyl | 87.7 % (64 of 73) | 78.0 % (32 of 41) | 100.0 % (32 of 32) |
1. Tn916 integrase
MSEKRRDNRG RILKTGESQR KDGRYLYKYI DSFGEPQFVY SWKLVATDRV PAGKRDAISL REKIAELQKD IHDG2. DNA binding site of Tn916 integrase
GAGTAGTAAA TTC3. DNA binding site of Tn916 integrase
GAATTTACTA CTCTemperature 300 (±1) K, pH 6.0 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Tn916 integrase | 1.0 mM | ||
2 | DNA binding site of Tn916 integrase | 1.0 mM | ||
3 | DNA binding site of Tn916 integrase | 1.0 mM |
Polymer type: polypeptide(L) polydeoxyribonucleotide
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 39.9 % (917 of 2300) | 37.3 % (545 of 1460) | 41.7 % (286 of 686) | 55.8 % (86 of 154) |
Backbone | 38.6 % (480 of 1244) | 33.7 % (225 of 668) | 40.3 % (174 of 432) | 56.3 % (81 of 144) |
Sidechain | 42.9 % (511 of 1192) | 40.5 % (321 of 792) | 47.4 % (185 of 390) | 50.0 % (5 of 10) |
Aromatic | 26.2 % (63 of 240) | 25.6 % (44 of 172) | 27.3 % (18 of 66) | 50.0 % (1 of 2) |
Methyl | 52.7 % (77 of 146) | 50.0 % (41 of 82) | 56.3 % (36 of 64) |
1. Tn916 integrase
MSEKRRDNRG RILKTGESQR KDGRYLYKYI DSFGEPQFVY SWKLVATDRV PAGKRDAISL REKIAELQKD IHDG2. DNA binding site of Tn916 integrase
GAGTAGTAAA TTC3. DNA binding site of Tn916 integrase
GAATTTACTA CTCTemperature 300 (±1) K, pH 6.0 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Tn916 integrase | 1.0 mM | ||
2 | DNA binding site of Tn916 integrase | 1.0 mM | ||
3 | DNA binding site of Tn916 integrase | 1.0 mM |
Polymer type: polypeptide(L) polydeoxyribonucleotide
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 31.8 % (1098 of 3450) | 32.1 % (703 of 2190) | 29.5 % (304 of 1029) | 39.4 % (91 of 231) |
Backbone | 30.5 % (570 of 1866) | 29.7 % (298 of 1002) | 28.7 % (186 of 648) | 39.8 % (86 of 216) |
Sidechain | 33.9 % (607 of 1788) | 34.3 % (407 of 1188) | 33.3 % (195 of 585) | 33.3 % (5 of 15) |
Aromatic | 25.0 % (90 of 360) | 27.5 % (71 of 258) | 18.2 % (18 of 99) | 33.3 % (1 of 3) |
Methyl | 39.7 % (87 of 219) | 41.5 % (51 of 123) | 37.5 % (36 of 96) |
1. Tn916 integrase
MSEKRRDNRG RILKTGESQR KDGRYLYKYI DSFGEPQFVY SWKLVATDRV PAGKRDAISL REKIAELQKD IHDG2. DNA binding site of Tn916 integrase
GAGTAGTAAA TTC3. DNA binding site of Tn916 integrase
GAATTTACTA CTCTemperature 300 (±1) K, pH 6.0 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Tn916 integrase | 1.0 mM | ||
2 | DNA binding site of Tn916 integrase | 1.0 mM | ||
3 | DNA binding site of Tn916 integrase | 1.0 mM |