Expression and Assignment of 1H, 15N,and 13C Resonances of the C-terminal Domain of the Diphtheria Toxin Repressor
MRGSHHHHHH GSNPIPGLDE LGVGNSDAAA PGTRVIDAAT SMPRKVRIVQ INEIFQVETD QFTQLLDADI RVGSEVEIVD RDGHITLSHN GKDVELLDDL AHTIRIEEL
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 83.0 % (998 of 1203) | 80.5 % (498 of 619) | 84.9 % (400 of 471) | 88.5 % (100 of 113) |
Backbone | 88.7 % (573 of 646) | 87.4 % (195 of 223) | 89.3 % (284 of 318) | 89.5 % (94 of 105) |
Sidechain | 77.6 % (510 of 657) | 75.8 % (300 of 396) | 80.6 % (204 of 253) | 75.0 % (6 of 8) |
Aromatic | 8.9 % (5 of 56) | 17.9 % (5 of 28) | 0.0 % (0 of 28) | |
Methyl | 90.6 % (125 of 138) | 88.4 % (61 of 69) | 92.8 % (64 of 69) |
1. DtxR subunit 3, C-terminal domain
MRGSHHHHHH GSNPIPGLDE LGVGNSDAAA PGTRVIDAAT SMPRKVRIVQ INEIFQVETD QFTQLLDADI RVGSEVEIVD RDGHITLSHN GKDVELLDDL AHTIRIEELTemperature 303 (±0.5) K, pH 6.5 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | DtxR subunit 3, C-terminal domain | [U-95% 15N] | 1.0 ~ 2.0 mM |
Temperature 303 (±0.5) K, pH 6.5 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
2 | DtxR subunit 3, C-terminal domain | [U-95% 15N; U-95% 13C] | 1.0 ~ 2.0 mM |