BMRB: 4186

NMR Solution Structure of Human Cellular Retinoic Acid Binding Protein-Type II

Authors

Wang, L., Li, Y., Abildgaard, F., Markley, J.L., Yan, H.

Assembly

CRABPII system

Entity

1. CRABPII system (polymer), 137 monomers, 15561.63 Da Detail

PNFSGNWKII RSENFEELLK VLGVNVMLRK IAVAAASKPA VEIKQEGDTF YIKTSTTVRT TEINFKVGEE FEEQTVDGRP CKSLVKWESE NKMVCEQKLL KGEGPKTSWT RELTNDGELI LTMTADDVVC TRVYVRE

Formula weight

15561.63 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

DGSA

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.1 %, Completeness: 73.0 %, Completeness (bb): 89.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	73.0 % (1182 of 1620)	81.4 % (687 of 844)	58.9 % (371 of 630)	84.9 % (124 of 146)
Backbone	89.2 % (726 of 814)	92.8 % (258 of 278)	85.4 % (344 of 403)	93.2 % (124 of 133)
Sidechain	60.7 % (568 of 935)	75.8 % (429 of 566)	39.0 % (139 of 356)	0.0 % (0 of 13)
Aromatic	4.9 % (5 of 102)	9.8 % (5 of 51)	0.0 % (0 of 48)	0.0 % (0 of 3)
Methyl	56.2 % (91 of 162)	96.3 % (78 of 81)	16.0 % (13 of 81)

1. Cellular Retinoic Acid Binding Protein-Type II

PNFSGNWKII RSENFEELLK VLGVNVMLRK IAVAAASKPA VEIKQEGDTF YIKTSTTVRT TEINFKVGEE FEEQTVDGRP CKSLVKWESE NKMVCEQKLL KGEGPKTSWT RELTNDGELI LTMTADDVVC TRVYVRE

Show sample condition

Sample

Temperature 298 (±0.5) K, pH 7.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Cellular Retinoic Acid Binding Protein-Type II			1.5 ~ 2.0 mM

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LACS Plot; CA

Referencing offset: -0.37 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	direct	0.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.37 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	direct	0.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.09 ppm, Outliers: 5 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	direct	0.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 3.21 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	direct	0.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 22 models in PDB: 1BLR, Strand ID: A Detail

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Release date

2000-03-07

Citation 1

NMR solution structure of type II human cellular retinoic acid binding protein: implications for ligand binding
Wang, L., Li, Y., Abildgaard, F., Markley, J.L., Yan, H.
Biochemistry (1998), 37, 12727-12736, PubMed 9737849 , DOI 10.1021/bi9808924 , Abstract

Show more 1 citaion

Citation 2

1H, 13C and 15N chemical shift referencing in biomolecular NMR
Wishart, D.S., Bigam, C.G., Yao, J., Abildgaard, F., Dyson, H.J., Oldfield, E., Markley, J.L., Sykes, B.D.
J. Biomol. NMR (1995), 6, 135-140, PubMed 8589602 , DOI: , Abstract

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Related entities 1. CRABPII system, : 1 : 15 : 5 : 4 : 247 entities Detail

Interaction partners 1. CRABPII system, : 3 interactors Detail

Experiments performed 3 experiments Detail