BMRB: 4204

Three-Dimensional Structure of Human Insulin-Like Growth Factor-I (IGF-I) Determined by 1H-NMR and Distance Geometry

Authors

Sato, A., Nishimura, S., Ohkubo, T., Kyogoku, Y., Koyama, S., Kobayashi, M., Kobayashi, T.Y.

Assembly

Insulin-like growth factor-I (Somatomedin C)

Entity

1. Insulin-like growth factor-I (Somatomedin C) (polymer), 70 monomers, 7654.677 Da Detail

GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA

Formula weight

7654.677 Da

Entity Connection

disulfide 3 Detail

---

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS6:SG	1:CYS48:SG
2	disulfide	sing	1:CYS18:SG	1:CYS61:SG
3	disulfide	sing	1:CYS47:SG	1:CYS52:SG

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

DISTANCE GEOMETRY

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 98.3 %, Completeness (bb): 100.0 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H
All	98.3 % (400 of 407)	98.3 % (400 of 407)
Backbone	100.0 % (142 of 142)	100.0 % (142 of 142)
Sidechain	97.4 % (258 of 265)	97.4 % (258 of 265)
Aromatic	78.1 % (25 of 32)	78.1 % (25 of 32)
Methyl	100.0 % (29 of 29)	100.0 % (29 of 29)

1. Insulin-like growth factor-I (Somatomedin C)

GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA

Show sample condition

Sample

Temperature 313 (±0.1) K, pH 3.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Insulin-like growth factor-I (Somatomedin C)			10 mM
2	D20			90 %
3	acetic acid	[U-2H]		10 %

Hide sample codition

Protein Blocks Logo

Calculated from 6 models in PDB: 1BQT, Strand ID: A Detail

---

Release date

1999-12-21

Citation

Three-dimensional structure of human insulin-like growth factor-I (IGF-I) determined by 1H-NMR and distance geometry
Sato, A., Nishumura, S., Ohkubo, T., Kyogoku, Y., Koyama, S., Kobatashi, M., Yasuda, T., Kobayashi, Y.
Int. J. Pept. Protein Res. (1993), 41, 433-440, PubMed 8391516 , Abstract

Related entities 1. Insulin-like growth factor-I (Somatomedin C), : 1 : 30 : 3 : 6 : 155 entities Detail

Interaction partners 1. Insulin-like growth factor-I (Somatomedin C), : 9 interactors Detail

Experiments performed 3 experiments Detail