BMRB: 4211

Ragweed pollen allergen from Ambrosia trifida V

Authors

Warren, G.L., Tuner, C.J., Petsko, G.A., Brunger, A.T.

Assembly

giant ragweed allergen 5

Entity

1. giant ragweed allergen 5 (polymer, Thiol state: all disulfide bound), 40 monomers, 4320.944 Da Detail

DDGLCYEGTN CGKVGKYCCS PIGKYCVCYD SKAICNKNCT

Formula weight

4320.944 Da

Entity Connection

disulfide 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS5:SG	1:CYS35:SG
2	disulfide	sing	1:CYS11:SG	1:CYS26:SG
3	disulfide	sing	1:CYS18:SG	1:CYS28:SG
4	disulfide	sing	1:CYS19:SG	1:CYS39:SG

Source organism

Ambrosia trifida

Exptl. method

NMR

Refine. method

ITERATIVE SIMULATED ANNEALING

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 99.5 %, Completeness (bb): 98.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	99.5 % (221 of 222)	99.5 % (221 of 222)
Backbone	98.8 % (83 of 84)	98.8 % (83 of 84)
Sidechain	100.0 % (138 of 138)	100.0 % (138 of 138)
Aromatic	100.0 % (16 of 16)	100.0 % (16 of 16)
Methyl	100.0 % (13 of 13)	100.0 % (13 of 13)

1. giant ragweed allergen 5

DDGLCYEGTN CGKVGKYCCS PIGKYCVCYD SKAICNKNCT

Show sample condition

Sample #1

Temperature 293 K, pH 4.0

#	Name	Isotope labeling	Type	Concentration
1	giant ragweed allergen 5			7.8 mM
2	acetate			50 mM
3	NaN3			1 mM
4	EDTA			11 mM
5	D2O			10 %
6	H2O			90 %

Sample #2

Temperature 293 K, pH 4.0

#	Name	Isotope labeling	Type	Concentration
7	giant ragweed allergen 5			7.8 mM
8	acetate			50 mM
9	NaN3			1 mM
10	EDTA			11 mM
11	D2O			99.9 %

Hide sample codition

Protein Blocks Logo

Calculated from 1 models in PDB: 1BBG, Strand ID: A Detail

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Coupling constant

32 J values in 1 lists
Temperature 293 K, pH 4.0 Detail

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Temperature 293 K, pH 4.0

List #1 J_three_bond_constraints_label

Release date

2007-06-18

Citation

A highly precise solution 1H NMR structure of ragweed allergen Amb. t. V
Warren, G.L., Turner, C.J., Petsko, G.A., Brunger, A.T.
J. Mol. Biol.

Related entities 1. giant ragweed allergen 5, : 1 : 3 : 23 entities Detail

Experiments performed 1 experiments Detail