BMRBj - BMREntryMaster

Found 1 Document (1.726 seconds)

BMRB: 4215

1SSN

Staphylokinase (NMR, Sakstar Variant)

Authors

Ohlenschlager, O., Ramachandran, R., Guhrs, K.H., Schlott, B., Brown, L.R.

Assembly

Staphylokinase

Entity

1. Staphylokinase (polymer, Thiol state: not present), 136 monomers, 15494.41 Da Detail

SSSFDKGKYK KGDDASYFEP TGPYLMVNVT GVDSKGNELL SPHYVEFPIK PGTTLTKEKI EYYVEWALDA TAYKEFRVVE LDPSAKIEVT YYDKNKKKEE TKSFPITEKG FVVPDLSEHI KNPGFNLITK VVIEKK

Formula weight

15494.41 Da

Source organism

Staphylococcus aureus

Exptl. method

NMR

Refine. method

DISTANCE GEOMETRY

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.6 %, Completeness (bb): 97.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	97.6 % (1610 of 1650)	98.5 % (849 of 862)	96.6 % (633 of 655)	96.2 % (128 of 133)
Backbone	97.0 % (774 of 798)	98.5 % (267 of 271)	96.2 % (385 of 400)	96.1 % (122 of 127)
Sidechain	98.4 % (964 of 980)	98.5 % (582 of 591)	98.2 % (376 of 383)	100.0 % (6 of 6)
Aromatic	95.1 % (154 of 162)	97.5 % (79 of 81)	92.5 % (74 of 80)	100.0 % (1 of 1)
Methyl	99.3 % (137 of 138)	98.6 % (68 of 69)	100.0 % (69 of 69)

1. Staphylokinase

SSSFDKGKYK KGDDASYFEP TGPYLMVNVT GVDSKGNELL SPHYVEFPIK PGTTLTKEKI EYYVEWALDA TAYKEFRVVE LDPSAKIEVT YYDKNKKKEE TKSFPITEKG FVVPDLSEHI KNPGFNLITK VVIEKK

Show sample condition

Sample

Temperature 300 (±1) K, pH 6.7 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Staphylokinase	[U-100% 15N]		1.10 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	0.0 ppm	external	null	0.0

Referencing offset: 1.85 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	0.0 ppm	external	null	0.0

Referencing offset: 1.85 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	0.0 ppm	external	null	0.0

Referencing offset: -0.04 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	0.0 ppm	external	null	0.0

Referencing offset: 2.18 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1SSN, Strand ID: A Detail

Release date

2000-10-01

Citation 1

Nuclear magnetic resonance solution structure of the plasminogen-activator protein staphylokinase
Ohlenschlager, O., Ramachandran, R., Guhrs, K.H., Schlott, B., Brown, L.R.
Biochemistry (1998), 37, 10635-10642, PubMed 9692953 , DOI 10.1021/bi980673i , Abstract

Show more 1 citaion

Citation 2

The program XEASY for computer-supported NMR spectral analysis of biological macromolecules
J. Biomol. NMR (1995), 6, 1-10, PubMed 22911575 , DOI 10.1007/BF00417486 , Abstract

Hide non-primary 1 citaion

Related entities 1. Staphylokinase, : 1 : 1 : 9 : 3 entities Detail

Interaction partners 1. Staphylokinase, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 15 experiments Detail

1 15N-1H HSQC

Instrument

Model Varian Inova (750 MHz)

Sample #1

Temperature 300 (±1) K, pH 6.7 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Staphylokinase	[U-100% 15N]		1.10 mM

Sample #2

Temperature 300 (±1) K, pH 6.7 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	Staphylokinase	[U-100% 13C;U-100% 15N]		1.28 mM

2 13C-1H HSQC

Instrument

Model Varian Inova (750 MHz)

Sample #1

Temperature 300 (±1) K, pH 6.7 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Staphylokinase	[U-100% 15N]		1.10 mM

Sample #2

Temperature 300 (±1) K, pH 6.7 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	Staphylokinase	[U-100% 13C;U-100% 15N]		1.28 mM

3 1H-15N-1H TOCSY-HSQC

Instrument

Model Varian Inova (750 MHz)

Sample #1

Temperature 300 (±1) K, pH 6.7 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Staphylokinase	[U-100% 15N]		1.10 mM

Sample #2

Temperature 300 (±1) K, pH 6.7 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	Staphylokinase	[U-100% 13C;U-100% 15N]		1.28 mM

4 1H-15N-1H NOESY-HSQC

Instrument

Model Varian Inova (750 MHz)

Sample #1

Temperature 300 (±1) K, pH 6.7 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Staphylokinase	[U-100% 15N]		1.10 mM

Sample #2

Temperature 300 (±1) K, pH 6.7 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	Staphylokinase	[U-100% 13C;U-100% 15N]		1.28 mM

5 1H-15N-1H HBHA(CBCACO)NH

Instrument

Model Varian Inova (750 MHz)

Sample #1

Temperature 300 (±1) K, pH 6.7 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Staphylokinase	[U-100% 15N]		1.10 mM

Sample #2

Temperature 300 (±1) K, pH 6.7 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	Staphylokinase	[U-100% 13C;U-100% 15N]		1.28 mM

6 15N-1H-1H HNHA

Instrument

Model Varian Inova (750 MHz)

Sample #1

Temperature 300 (±1) K, pH 6.7 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Staphylokinase	[U-100% 15N]		1.10 mM

Sample #2

Temperature 300 (±1) K, pH 6.7 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	Staphylokinase	[U-100% 13C;U-100% 15N]		1.28 mM

7 15N-15N-1H HSQC-NOESY-HSQC

Instrument

Model Varian Inova (750 MHz)

Sample #1

Temperature 300 (±1) K, pH 6.7 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Staphylokinase	[U-100% 15N]		1.10 mM

Sample #2

Temperature 300 (±1) K, pH 6.7 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	Staphylokinase	[U-100% 13C;U-100% 15N]		1.28 mM

8 1H-13C-1H HCCH-TOCSY

Instrument

Model Varian Inova (750 MHz)

Sample #1

Temperature 300 (±1) K, pH 6.7 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Staphylokinase	[U-100% 15N]		1.10 mM

Sample #2

Temperature 300 (±1) K, pH 6.7 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	Staphylokinase	[U-100% 13C;U-100% 15N]		1.28 mM

9 1H-13C-1H NOESY-HSQC

Instrument

Model Varian Inova (750 MHz)

Sample #1

Temperature 300 (±1) K, pH 6.7 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Staphylokinase	[U-100% 15N]		1.10 mM

Sample #2

Temperature 300 (±1) K, pH 6.7 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	Staphylokinase	[U-100% 13C;U-100% 15N]		1.28 mM

10 15N-13C-1H HNCO

Instrument

Model Varian Inova (750 MHz)

Sample #1

Temperature 300 (±1) K, pH 6.7 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Staphylokinase	[U-100% 15N]		1.10 mM

Sample #2

Temperature 300 (±1) K, pH 6.7 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	Staphylokinase	[U-100% 13C;U-100% 15N]		1.28 mM

11 15N-13C-1H HNCACB

Instrument

Model Varian Inova (750 MHz)

Sample #1

Temperature 300 (±1) K, pH 6.7 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Staphylokinase	[U-100% 15N]		1.10 mM

Sample #2

Temperature 300 (±1) K, pH 6.7 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	Staphylokinase	[U-100% 13C;U-100% 15N]		1.28 mM

12 13C-15N-1H CBCA(CO)NH

Instrument

Model Varian Inova (750 MHz)

Sample #1

Temperature 300 (±1) K, pH 6.7 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Staphylokinase	[U-100% 15N]		1.10 mM

Sample #2

Temperature 300 (±1) K, pH 6.7 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	Staphylokinase	[U-100% 13C;U-100% 15N]		1.28 mM

13 13C-15N-1H CC(CO)NH

Instrument

Model Varian Inova (750 MHz)

Sample #1

Temperature 300 (±1) K, pH 6.7 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Staphylokinase	[U-100% 15N]		1.10 mM

Sample #2

Temperature 300 (±1) K, pH 6.7 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	Staphylokinase	[U-100% 13C;U-100% 15N]		1.28 mM

14 13C-1H-15N-1H HMQC-NOESY-HSQC

Instrument

Model Varian Inova (750 MHz)

Sample #1

Temperature 300 (±1) K, pH 6.7 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Staphylokinase	[U-100% 15N]		1.10 mM

Sample #2

Temperature 300 (±1) K, pH 6.7 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	Staphylokinase	[U-100% 13C;U-100% 15N]		1.28 mM

15 13C-1H-13C-1H HMQC-NOESY-HSQC

Instrument

Model Varian Inova (750 MHz)

Sample #1

Temperature 300 (±1) K, pH 6.7 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Staphylokinase	[U-100% 15N]		1.10 mM

Sample #2

Temperature 300 (±1) K, pH 6.7 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	Staphylokinase	[U-100% 13C;U-100% 15N]		1.28 mM

nullKeywords Plasminogen-activator, Staphylokinase

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Found 1 Document (1.726 seconds)

BMRB: 4215

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Staphylokinase, : 1 : 1 : 9 : 3 entities Detail

Interaction partners 1. Staphylokinase, : 1 interactors Detail

Experiments performed 15 experiments Detail

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