BMRB: 4222

High-Resolution Solution Structure of a Sweet Protein Single-Chain Monellin (SCM) determined by Nuclear Magnetic Resonance Spectroscopy and Dynamical Simulated Annealing Calculations

Authors

Lee, S., Lee, J., Chang, H., Cho, J., Jung, J., Lee, W.

Assembly

monellin

Entity

1. monellin (polymer, Thiol state: not reported), 94 monomers, 11067.49 Da Detail

GEWEIIDIGP FTQNLGKFAV DEENKIGQYG RLTFNKVIRP CMKKTIYENE REIKGYEYQL YVYASDKLFR ADISEDYKTR GRKLLRFNGP VPPP

Formula weight

11067.49 Da

Source organism

Dioscoreophyllum cumminsii

Exptl. method

NMR

Refine. method

HYBRID GEOMETRY/DYNAMICAL SIMULATED ANNEALING CALCULATIONS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.7 %, Completeness: 77.1 %, Completeness (bb): 96.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	77.1 % (553 of 717)	75.2 % (466 of 620)	89.7 % (87 of 97)
Backbone	96.0 % (267 of 278)	95.8 % (182 of 190)	96.6 % (85 of 88)
Sidechain	65.1 % (286 of 439)	66.0 % (284 of 430)	22.2 % (2 of 9)
Aromatic	60.0 % (36 of 60)	61.0 % (36 of 59)	0.0 % (0 of 1)
Methyl	55.8 % (24 of 43)	55.8 % (24 of 43)

1. monellin

GEWEIIDIGP FTQNLGKFAV DEENKIGQYG RLTFNKVIRP CMKKTIYENE REIKGYEYQL YVYASDKLFR ADISEDYKTR GRKLLRFNGP VPPP

Show sample condition

Sample

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	monellin			0.0 ~ 0.0 mM

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Protein Blocks Logo

Calculated from 21 models in PDB: 1MNL, Strand ID: A Detail

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Release date

2000-04-30

Citation

Solution structure of a sweet protein single-chain monellin determined by nuclear magnetic resonance and dynamical simulated annealing calculations
Lee, S., Lee, J., Chang, H., Cho, J., Jung, J., Lee, W.
Biochemistry (1999), 38, 2340-2346, PubMed 10029527 , DOI 10.1021/bi9822731 , Abstract

Related entities 1. monellin, : 1 : 1 : 2 : 42 entities Detail

Experiments performed 7 experiments Detail