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High-Resolution Solution Structure of a Sweet Protein Single-Chain Monellin (SCM) determined by Nuclear Magnetic Resonance Spectroscopy and Dynamical Simulated Annealing Calculations
Authors
Lee, S., Lee, J., Chang, H., Cho, J., Jung, J., Lee, W.
Assembly
monellin
Entity
1. monellin (polymer, Thiol state: not reported), 94 monomers, 11067.49 Da Detail

GEWEIIDIGP FTQNLGKFAV DEENKIGQYG RLTFNKVIRP CMKKTIYENE REIKGYEYQL YVYASDKLFR ADISEDYKTR GRKLLRFNGP VPPP


Formula weight
11067.49 Da
Source organism
Dioscoreophyllum cumminsii
Exptl. method
NMR
Refine. method
HYBRID GEOMETRY/DYNAMICAL SIMULATED ANNEALING CALCULATIONS
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 96.8 %, Completeness: 76.5 %, Completeness (bb): 98.5 % Detail

Polymer type: polypeptide(L)

Total1H15N
All76.5 % (554 of 724)75.3 % (467 of 620)83.7 % (87 of 104)
Backbone98.5 % (266 of 270)98.4 % (179 of 182)98.9 % (87 of 88)
Sidechain63.4 % (288 of 454)65.8 % (288 of 438) 0.0 % (0 of 16)
Aromatic60.0 % (36 of 60)61.0 % (36 of 59) 0.0 % (0 of 1)
Methyl55.8 % (24 of 43)55.8 % (24 of 43)

1. monellin

GEWEIIDIGP FTQNLGKFAV DEENKIGQYG RLTFNKVIRP CMKKTIYENE REIKGYEYQL YVYASDKLFR ADISEDYKTR GRKLLRFNGP VPPP

Sample

Temperature 298 K, pH 7.0


#NameIsotope labelingTypeConcentration
1monellin0.0 ~ 0.0 mM

Protein Blocks Logo
Calculated from 21 models in PDB: 1MNL, Strand ID: A Detail


Release date
2000-04-30
Citation
Solution structure of a sweet protein single-chain monellin determined by nuclear magnetic resonance and dynamical simulated annealing calculations
Lee, S., Lee, J., Chang, H., Cho, J., Jung, J., Lee, W.
Biochemistry (1999), 38, 2340-2346, PubMed 10029527 , DOI 10.1021/bi9822731 ,
Related entities 1. monellin, : 1 : 1 : 25 entities Detail
Keywords alpha/beta motif, sweet protein, sweet receptor binding