BMRB: 4223

Solution Structure of a TBP-TAFII230 Complex: Protein Mimicry of the Minor Groove Surface of the TATA Box Unwound by TBP

Authors

Liu, D., Ishima, R., Tong, K.I., Baby, S., Kokubo, T., Muhandiram, D.R., Kay, L.E., Nakatani, Y., Ikura, M.

Assembly

TATA box binding protein associated factor II 230

Entity

1. TATA box binding protein associated factor II 230 (polymer), 67 monomers, 7007.630 Da Detail

EGSIGNGLDL TGILFGNIDS EGRLLQDDDG EGRGGTGFDA ELRENIGSLS KLGLDSMLLE VIDLKEA

Formula weight

7007.63 Da

Source organism

Drosophila

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.5 %, Completeness: 88.1 %, Completeness (bb): 90.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	88.1 % (630 of 715)	87.3 % (322 of 369)	87.3 % (240 of 275)	95.8 % (68 of 71)
Backbone	90.8 % (365 of 402)	89.8 % (132 of 147)	89.9 % (169 of 188)	95.5 % (64 of 67)
Sidechain	86.1 % (316 of 367)	85.6 % (190 of 222)	86.5 % (122 of 141)	100.0 % (4 of 4)
Aromatic	50.0 % (10 of 20)	100.0 % (10 of 10)	0.0 % (0 of 10)
Methyl	100.0 % (80 of 80)	100.0 % (40 of 40)	100.0 % (40 of 40)

1. TATA box binding protein associated factor

EGSIGNGLDL TGILFGNIDS EGRLLQDDDG EGRGGTGFDA ELRENIGSLS KLGLDSMLLE VIDLKEA

Show sample condition

Sample #1

Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	TATA box binding protein associated factor	[U-13C; U-15N; U60%-2H]		1.0 mM

Sample #2

Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	TATA box binding protein associated factor	[U-13C; U-15N]		1.0 mM

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LACS Plot; CA

Referencing offset: -0.24 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.24 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.14 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.07 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 25 models in PDB: 1TBA, Strand ID: A Detail

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Release date

1999-12-05

Citation

Solution Structure of a TBP-TAFII230 Complex: Protein Mimicry of the Minor Groove Surface of the TATA Box Unwound by TB
Liu, D., Ishima, R., Tong, K.I., Bagby, S., Kokubo, T., Muhandiram, D.R., Kay, L.E., Nakatani, Y., Ikura, M.
Cell (1998), 94, 573-583, PubMed , DOI:

Related entities 1. TATA box binding protein associated factor II 230, : 1 : 2 : 2 entities Detail

Interaction partners 1. TATA box binding protein associated factor II 230, : 19 interactors Detail

Experiments performed 1 experiments Detail