BMRB: 4235

NMR Solution Structure of [d(GCGAATTCGC)2]

Authors

Aramini, J.M., Mujeeb, A., Germann, M.W.

Assembly

DNA decamer

Entity

1. DNA decamer (polymer), 10 monomers, 3107.976 × 2 Da Detail

GCGAATTCGC

Total weight

6215.952 Da

Max. entity weight

3107.976 Da

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 80.6 %, Completeness (bb): 81.3 % Detail

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Polymer type: polydeoxyribonucleotide

	Total	1H	31P
All	80.6 % (87 of 108)	79.6 % (78 of 98)	90.0 % (9 of 10)
Suger, PO4	81.3 % (65 of 80)	80.0 % (56 of 70)	90.0 % (9 of 10)
Nucleobase	78.6 % (22 of 28)	78.6 % (22 of 28)
Aromatic	100.0 % (20 of 20)	100.0 % (20 of 20)
Methyl	100.0 % (2 of 2)	100.0 % (2 of 2)

1. DNA decamer

GCGAATTCGC

Show sample condition

Sample

Pressure 1 atm, Temperature 303 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	DNA decamer			2.6 mM
2	NaCl			50 mM
3	NaPhosphate			10 mM
4	EDTA			0.1 mM

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Release date

1999-02-07

Citation

NMR Solution Structures of [d(GCGAAT-3'-3'-alphaT-5'-5'-CGC)2] and its Unmodified Control
Aramini, J.M., Mujeeb, A., Germann, M.W.
Nucleic Acids Res. (1998), 15, 5644-5654, PubMed , DOI:

Experiments performed 3 experiments Detail