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Assessing Potential Bias in the Determination of Rotational Correlation Times of Proteins by NMR Relaxation
Authors
Lee, A.L., Wand, A.J.
Assembly
ubiquitin
Entity
1. ubiquitin (polymer, Thiol state: not present), 76 monomers, 8564.731 Da Detail

MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG


Formula weight
8564.731 Da
Source organism
Homo sapiens
Exptl. method
NMR
Data set
heteronucl_NOEs, heteronucl_T1_relaxation, heteronucl_T2_relaxation, order_parameters, heteronucl_T1rho_relaxation
Heteronucl. T1
248 T1 values in 4 lists
Coherence Sz, Field strength (1H) 750 MHz, 400 MHz, 500 MHz, 600 MHz, Temperature 298 (±0.2) K, pH 5.0 (±0.05) Detail
Heteronucl. T2
124 T2 values in 2 lists
Coherence S(+,-), Field strength (1H) 500 MHz, 600 MHz, Temperature 298 (±0.2) K, pH 5.0 (±0.05) Detail
Heteronucl. NOE
186 NOE values in 3 lists
Value type na, Field strength (1H) 750 MHz, 500 MHz, 600 MHz, Temperature 298 (±0.2) K, pH 5.0 (±0.05) Detail
Heteronucl. T1/T2
124 T1/T2 values in 2 lists
Field strength (1H) 500 MHz, 600 MHz, Temperature 298 (±0.2) K, pH 5.0 (±0.05) Detail
Order parameters
51 S2 values in 1 lists
Temperature 298 (±0.2) K, pH 5.0 (±0.05) Detail
Release date
1999-09-14
Citation 1
Assessing potential bias in the determination of rotational correlation times of proteins by NMR relaxation
Lee, A.L., Wand, A.J.
J. Biomol. NMR (1999), 13, 101-112, PubMed 10070752 ,
Citation 2
1H, 13C and 15N chemical shift referencing in biomolecular NMR
Wishart, D.S., Bigam, C.G., Yao, J., Abildgaard, F., Dyson, H.J., Oldfield, E., Markley, J.L., Sykes, B.D.
J. Biomol. NMR (1995), 6, 135-140, PubMed 8589602 , DOI 10.1007/bf00211777 ,
Related entities 1. ubiquitin, : 1 : 200 : 5 : 25 : 140 entities Detail
Interaction partners 1. ubiquitin, : 27 interactors Detail
Experiments performed 4 experiments Detail
Keywords 15N relaxation, chemical shift anisotropy, correlation time, nuclear Overhauser effect, protein NMR, spin-lattice relaxation, spin-spin relaxation