BMRB: 4245

Assessing Potential Bias in the Determination of Rotational Correlation Times of Proteins by NMR Relaxation

Authors

Lee, A.L., Wand, A.J.

Assembly

ubiquitin

Entity

1. ubiquitin (polymer, Thiol state: not present), 76 monomers, 8564.731 Da Detail

MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG

Formula weight

8564.731 Da

Source organism

Homo sapiens

Exptl. method

NMR

Data set

heteronucl_NOEs, heteronucl_T1_relaxation, heteronucl_T2_relaxation, order_parameters, heteronucl_T1rho_relaxation

Heteronucl. T₁

248 T₁ values in 4 lists
Coherence Sz, Field strength (¹H) 750 MHz, 400 MHz, 500 MHz, 600 MHz, Temperature 298 (±0.2) K, pH 5.0 (±0.05) Detail

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Temperature 298 (±0.2) K, pH 5.0 (±0.05)

Heteronucl. T₂

124 T₂ values in 2 lists
Coherence S(+,-), Field strength (¹H) 500 MHz, 600 MHz, Temperature 298 (±0.2) K, pH 5.0 (±0.05) Detail

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Temperature 298 (±0.2) K, pH 5.0 (±0.05)

Heteronucl. NOE

186 NOE values in 3 lists
Value type na, Field strength (¹H) 750 MHz, 500 MHz, 600 MHz, Temperature 298 (±0.2) K, pH 5.0 (±0.05) Detail

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Temperature 298 (±0.2) K, pH 5.0 (±0.05)

Heteronucl. T₁/T₂

124 T₁/T₂ values in 2 lists
Field strength (¹H) 500 MHz, 600 MHz, Temperature 298 (±0.2) K, pH 5.0 (±0.05) Detail

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Temperature 298 (±0.2) K, pH 5.0 (±0.05)

Order parameters

51 S² values in 1 lists
Temperature 298 (±0.2) K, pH 5.0 (±0.05) Detail

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Temperature 298 (±0.2) K, pH 5.0 (±0.05)

List #1 S2_parameters, Details fitted from T1 data at 41, 51, 61, and 76 MHz 15N frequencies and from {1H}-15N data at 51, 61, and 76 MHz 15N frequencies overall correlation time, tau-m, was fixed at 4.1 ns

Release date

1999-09-14

Citation 1

Assessing potential bias in the determination of rotational correlation times of proteins by NMR relaxation
Lee, A.L., Wand, A.J.
J. Biomol. NMR (1999), 13, 101-112, PubMed 10070752 , Abstract

Show more 1 citaion

Citation 2

1H, 13C and 15N chemical shift referencing in biomolecular NMR
Wishart, D.S., Bigam, C.G., Yao, J., Abildgaard, F., Dyson, H.J., Oldfield, E., Markley, J.L., Sykes, B.D.
J. Biomol. NMR (1995), 6, 135-140, PubMed 8589602 , DOI: , Abstract

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Related entities 1. ubiquitin, : 1 : 292 : 11 : 71 : 321 entities Detail

Interaction partners 1. ubiquitin, : 27 interactors Detail

Experiments performed 4 experiments Detail