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2LEF

LEF1 HMG Domain (From Mouse), Complexed with DNA (15bp), NMR, 12 Structures

Authors

Li, X., Love, J.J., Case, D.A., Wright, P.E.

Assembly

Lymphoid enhancer-binding factor

Entity

1. Lymphoid enhancer-binding factor (polymer), 86 monomers, 10379.02 Da Detail

MHIKKPLNAF MLYMKEMRAN VVAESTLKES AAINQILGRR WHALSREEQA KYYELARKER QLHMQLYPGW SARDNYGKKK KRKREK

2. DNA (polymer), 15 monomers, 4567.908 Da Detail

CACCCTTTGA AGCTC

3. DNA (polymer), 15 monomers, 4737.018 Da Detail

GAGCTTCAAA GGGTG

Total weight

19683.945 Da

Max. entity weight

10379.02 Da

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

DG IN TORSION SPACE (DIANA) AND DYNAMICAL SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 74.1 %, Completeness: 64.9 %, Completeness (bb): 59.4 % Detail

Polymer type: polypeptide(L) polydeoxyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	64.9 % (901 of 1389)	56.3 % (496 of 881)	76.3 % (316 of 414)	94.7 % (89 of 94)
Backbone	59.4 % (429 of 722)	44.6 % (171 of 383)	69.0 % (176 of 255)	97.6 % (82 of 84)
Sidechain	74.1 % (556 of 750)	65.5 % (326 of 498)	92.1 % (223 of 242)	70.0 % (7 of 10)
Aromatic	35.6 % (52 of 146)	26.2 % (27 of 103)	56.1 % (23 of 41)	100.0 % (2 of 2)
Methyl	91.1 % (72 of 79)	83.7 % (36 of 43)	100.0 % (36 of 36)

1. Lymphoid enhancer-binding factor

MHIKKPLNAF MLYMKEMRAN VVAESTLKES AAINQILGRR WHALSREEQA KYYELARKER QLHMQLYPGW SARDNYGKKK KRKREK

2. DNA

CACCCTTTGA AGCTC

3. DNA

GAGCTTCAAA GGGTG

Show sample condition

Sample

Pressure 1 atm, Temperature 303 (±1) K, pH 6.7 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Lymphoid enhancer-binding factor	[U-95% 13C; U-95% 15N]		1.2 mM

Chem. Shift Complete2

Sequence coverage: 22.4 %, Completeness: 39.6 %, Completeness (bb): 39.2 % Detail

Polymer type: polypeptide(L) polydeoxyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	39.6 % (1101 of 2778)	37.5 % (660 of 1762)	41.3 % (342 of 828)	52.7 % (99 of 188)
Backbone	39.2 % (566 of 1444)	36.3 % (278 of 766)	38.4 % (196 of 510)	54.8 % (92 of 168)
Sidechain	41.7 % (625 of 1500)	38.5 % (383 of 996)	48.6 % (235 of 484)	35.0 % (7 of 20)
Aromatic	28.1 % (82 of 292)	27.7 % (57 of 206)	28.0 % (23 of 82)	50.0 % (2 of 4)
Methyl	50.6 % (80 of 158)	48.8 % (42 of 86)	52.8 % (38 of 72)

1. Lymphoid enhancer-binding factor

MHIKKPLNAF MLYMKEMRAN VVAESTLKES AAINQILGRR WHALSREEQA KYYELARKER QLHMQLYPGW SARDNYGKKK KRKREK

2. DNA

CACCCTTTGA AGCTC

3. DNA

GAGCTTCAAA GGGTG

Show sample condition

Sample

Pressure 1 atm, Temperature 303 (±1) K, pH 6.7 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Lymphoid enhancer-binding factor	[U-95% 13C; U-95% 15N]		1.2 mM

Chem. Shift Complete3

Sequence coverage: 22.4 %, Completeness: 30.8 %, Completeness (bb): 32.2 % Detail

Polymer type: polypeptide(L) polydeoxyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	30.8 % (1285 of 4167)	30.4 % (804 of 2643)	30.0 % (373 of 1242)	38.3 % (108 of 282)
Backbone	32.2 % (697 of 2166)	32.7 % (376 of 1149)	28.8 % (220 of 765)	40.1 % (101 of 252)
Sidechain	30.4 % (685 of 2250)	28.7 % (429 of 1494)	34.3 % (249 of 726)	23.3 % (7 of 30)
Aromatic	23.5 % (103 of 438)	25.2 % (78 of 309)	18.7 % (23 of 123)	33.3 % (2 of 6)
Methyl	39.7 % (94 of 237)	40.3 % (52 of 129)	38.9 % (42 of 108)

1. Lymphoid enhancer-binding factor

MHIKKPLNAF MLYMKEMRAN VVAESTLKES AAINQILGRR WHALSREEQA KYYELARKER QLHMQLYPGW SARDNYGKKK KRKREK

2. DNA

CACCCTTTGA AGCTC

3. DNA

GAGCTTCAAA GGGTG

Show sample condition

Sample

Pressure 1 atm, Temperature 303 (±1) K, pH 6.7 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Lymphoid enhancer-binding factor	[U-95% 13C; U-95% 15N]		1.2 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	null	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 2.23 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	null	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 2.23 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	null	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 12 models in PDB: 2LEF, Strand ID: A Detail

Release date

1999-11-22

Citation

Structural basis for DNA bending by the architectural transcription factor LEF-1
Love, J.J., Li, X., Case, D.A., Giese, K., Grosschedl, R., Wright, P.E.
Nature (1995), 376, 791-795, PubMed 7651541 , DOI 10.1038/376791a0 , Abstract

Related entities 1. Lymphoid enhancer-binding factor, : 1 : 8 : 213 entities Detail

Experiments performed 18 experiments Detail

1 CT-HNCA