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Triple Resonance Assignment for Abl SH(32) and One in the Complex with a Consolidated Ligand
Authors
Xu, R., Cahill, S., Cowburn, D.
Assembly
Src homology domain 3 and 2
Entity
1. Src homology domain 3 and 2 (polymer), 166 monomers, 18296.00 Da Detail

GSPGGSLFVA LYDFVASGDN TLSITKGEKL RVLGYNHNGE WAEAQTKNGQ GWVPSNYITP VNSLEKHSWY HGPVSRNAAE YLLSSGINGS FLVRESESSP GQRSISLRYE GRVYHYRINT ASDGKLYVSS ESRFNTLAEL VHHHSTVADG LITTLHYPAP KRGIRD


Formula weight
18296.0 Da
Source organism
Homo sapiens
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 99.4 %, Completeness: 74.2 %, Completeness (bb): 79.7 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All74.2 % (1400 of 1886)81.2 % (785 of 967)63.5 % (466 of 734)80.5 % (149 of 185)
Backbone79.7 % (769 of 965)91.4 % (297 of 325)67.6 % (325 of 481)92.5 % (147 of 159)
Sidechain72.6 % (777 of 1070)76.0 % (488 of 642)71.4 % (287 of 402) 7.7 % (2 of 26)
Aromatic 5.3 % (10 of 188) 5.3 % (5 of 94) 5.5 % (5 of 91) 0.0 % (0 of 3)
Methyl94.7 % (161 of 170)95.3 % (81 of 85)94.1 % (80 of 85)

1. Src homology domain 3 and 2

GSPGGSLFVA LYDFVASGDN TLSITKGEKL RVLGYNHNGE WAEAQTKNGQ GWVPSNYITP VNSLEKHSWY HGPVSRNAAE YLLSSGINGS FLVRESESSP GQRSISLRYE GRVYHYRINT ASDGKLYVSS ESRFNTLAEL VHHHSTVADG LITTLHYPAP KRGIRD

Sample

Temperature 308 (±0.1) K, pH 7.2 (±0.5)


#NameIsotope labelingTypeConcentration
1Src homology domain 3 and 20.8 mM

LACS Plot; CA
Referencing offset: 0.02 ppm, Outliers: 2 Detail
LACS Plot; CB
Referencing offset: 0.02 ppm, Outliers: 2 Detail
LACS Plot; HA
Referencing offset: 0.0 ppm, Outliers: 1 Detail
Release date
1998-10-18
Citation 1
Triple resonance-based assignment for Abl SH(32) and its complex with a consolidated ligand
Xu, R., Cahill, S., Cowburn, D.
J. Biomol. NMR (1999), 14, 187-188, PubMed 10427746 ,
Citation 2
No title is available
Wishart, D.
Entries sharing articles BMRB: 1 entries Detail
  BMRB: 4252 released on 2007-07-12
    Title Triple Resonance Assignment for Abl SH(32) and One in the Complex with a Consolidated Ligand
Related entities 1. Src homology domain 3 and 2, : 2 : 143 entities Detail
Interaction partners 1. Src homology domain 3 and 2, : 19 interactors Detail
Experiments performed 1 experiments Detail
Keywords Abl_SH(32), NMR_assignment, consolidated_ligand