Triple Resonance Assignment for Abl SH(32) and One in the Complex with a Consolidated Ligand
GSPGGSLFVA LYDFVASGDN TLSITKGEKL RVLGYNHNGE WAEAQTKNGQ GWVPSNYITP VNSLEKHSWY HGPVSRNAAE YLLSSGINGS FLVRESESSP GQRSISLRYE GRVYHYRINT ASDGKLYVSS ESRFNTLAEL VHHHSTVADG LITTLHYPAP KRGIRD
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 63.3 % (1327 of 2097) | 69.0 % (747 of 1083) | 52.1 % (429 of 823) | 79.1 % (151 of 191) |
Backbone | 70.0 % (770 of 1100) | 81.8 % (315 of 385) | 56.4 % (305 of 541) | 86.2 % (150 of 174) |
Sidechain | 59.6 % (692 of 1162) | 62.2 % (434 of 698) | 57.5 % (257 of 447) | 5.9 % (1 of 17) |
Aromatic | 7.4 % (15 of 204) | 12.7 % (13 of 102) | 2.0 % (2 of 99) | 0.0 % (0 of 3) |
Methyl | 83.3 % (155 of 186) | 83.9 % (78 of 93) | 82.8 % (77 of 93) |
1. Src homology domain 3 and 2
GSPGGSLFVA LYDFVASGDN TLSITKGEKL RVLGYNHNGE WAEAQTKNGQ GWVPSNYITP VNSLEKHSWY HGPVSRNAAE YLLSSGINGS FLVRESESSP GQRSISLRYE GRVYHYRINT ASDGKLYVSS ESRFNTLAEL VHHHSTVADG LITTLHYPAP KRGIRD2. Abl SH3 binding peptide
PPAYAPPPVP3. Abl SH2 binding peptide
PVYENV4. G6-Kamide linker
GGGGGGTemperature 308 (±0.1) K, pH 7.2 (±0.5)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Src homology domain 3 and 2 | 0.8 mM |