BMRB: 4260

Effects of Glycosylation on the Structure and Dynamics of Eel Calcitonin in Micelles and Lipid Bilayers Determined by NMR Spectroscopy

Authors

Hashimoto, Y., Toma, K., Nishikido, J., Yamamoto, K., Haneda, K., Inazu, T., Valentine, K.G., Opella, S.J.

Assembly

GlcNAc eel calcitonin

Entity

1. GlcNAc eel calcitonin (polymer), 32 monomers, 3416.881 Da Detail

CSNLSTCVLG KLSQELHKLQ TYPRTDVGAG TX

Formula weight

3416.881 Da

Entity Connection

disulfide 1, covalent 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS1:SG	1:CYS7:SG
2	covalent	sing	1:ASN3:ND2	2:NAG1:C1

Source organism

Anguilla

Exptl. method

NMR

Refine. method

DISTANCE GEOMETRY

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.9 %, Completeness: 93.7 %, Completeness (bb): 96.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	93.7 % (164 of 175)	93.7 % (164 of 175)
Backbone	96.9 % (62 of 64)	96.9 % (62 of 64)
Sidechain	91.9 % (102 of 111)	91.9 % (102 of 111)
Aromatic	83.3 % (5 of 6)	83.3 % (5 of 6)
Methyl	94.7 % (18 of 19)	94.7 % (18 of 19)

1. GlcNAc eel calcitonin

CSNLSTCVLG KLSQELHKLQ TYPRTDVGAG TX

Show sample condition

Sample

Temperature 308 (±0.1) K, Details Sodium dodecyl sulfate-d25 was used as micelles

#	Name	Isotope labeling	Type	Concentration
1	GlcNAc eel calcitonin			1 mM

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Protein Blocks Logo

Calculated from 10 models in PDB: 1BYV, Strand ID: A Detail

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Release date

2000-05-21

Citation

Effects of glycosylation on the structure and dynamics of eel calcitonin in micelles and lipid bilayers determined by nuclear magnetic resonance spectroscopy
Hashimoto, Y., Toma, K., Nishikido, J., Yamamoto, K., Haneda, K., Inazu, T., Valentine, K.G., Opella, S.J.
Biochemistry (1999), 38, 8377-8384, PubMed 10387083 , DOI 10.1021/bi983018j , Abstract

Related entities 1. GlcNAc eel calcitonin, : 1 : 3 : 42 entities Detail

Experiments performed 3 experiments Detail