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Found 1 Document (1.938 seconds)

BMRB: 4264

1VRC

Complex of the amino terminal domain of enzyme I and the histidine-containing phosphocarrier protein HPr from Escherichia coli nmr, restrained regularized mean structure

Authors

Clore, G.M., Garrett, D.S., Gronenborn, A.M.

Assembly

Phosphotransferase system, enzyme I

Entity

1. Enzyme I, N-terminal (polymer, Thiol state: not present), 259 monomers, 28345.97 Da Detail

MISGILASPG IAFGKALLLK EDEIVIDRKK ISADQVDQEV ERFLSGRAKA SAQLETIKTK AGETFGEEKE AIFEGHIMLL EDEELEQEII ALIKDKHMTA DAAAHEVIEG QASALEELDD EYLKERAADV RDIGKRLLRN ILGLKIIDLS AIQDEVILVA ADLTPSETAQ LNLKKVLGFI TDAGGRTSHT SIMARSLELP AIVGTGSVTS QVKNDDYLIL DAVNNQVYVN PTNEVIDKMR AVQEQVASEK AELAKLKDR

2. Histidine Phosphocarrier (polymer, Thiol state: not present), 85 monomers, 9119.249 Da Detail

MFQQEVTITA PNGLHTRPAA QFVKEAKGFT SEITVTSNGK SASAKSLFKL QTLGLTQGTV VTISAEGEDE QKAVEHLVKL MAELE

Total weight

37465.22 Da

Max. entity weight

28345.97 Da

Source organism

Escherichia coli

Exptl. method

NMR

Refine. method

SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 82.6 %, Completeness: 64.5 %, Completeness (bb): 75.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	64.5 % (2522 of 3913)	59.8 % (1209 of 2022)	67.2 % (1026 of 1527)	78.8 % (287 of 364)
Backbone	75.4 % (1547 of 2052)	75.8 % (533 of 703)	73.3 % (741 of 1011)	80.8 % (273 of 338)
Sidechain	56.5 % (1233 of 2184)	51.3 % (676 of 1319)	64.7 % (543 of 839)	53.8 % (14 of 26)
Aromatic	34.8 % (48 of 138)	37.7 % (26 of 69)	31.9 % (22 of 69)
Methyl	67.9 % (322 of 474)	68.4 % (162 of 237)	67.5 % (160 of 237)

1. Enzyme I, N-terminal

2. Histidine Phosphocarrier

MFQQEVTITA PNGLHTRPAA QFVKEAKGFT SEITVTSNGK SASAKSLFKL QTLGLTQGTV VTISAEGEDE QKAVEHLVKL MAELE

Show sample condition

Sample

Temperature 313 K, pH 7.0

#	Name	Concentration
1	Enzyme I, N-terminal	0.0 ~ 0.0 mM
2	Histidine Phosphocarrier	0.0 ~ 0.0 mM
3	D2O	0.0 ~ 0.0 %
4	H2O	0.0 ~ 0.0 %

Chem. Shift Complete2

Sequence coverage: 59.9 %, Completeness: 53.3 %, Completeness (bb): 63.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	53.3 % (4174 of 7826)	49.9 % (2018 of 4044)	54.8 % (1673 of 3054)	66.3 % (483 of 728)
Backbone	63.3 % (2598 of 4104)	64.1 % (901 of 1406)	61.0 % (1233 of 2022)	68.6 % (464 of 676)
Sidechain	45.7 % (1996 of 4368)	42.3 % (1117 of 2638)	51.3 % (860 of 1678)	36.5 % (19 of 52)
Aromatic	26.1 % (72 of 276)	29.0 % (40 of 138)	23.2 % (32 of 138)
Methyl	53.1 % (503 of 948)	53.6 % (254 of 474)	52.5 % (249 of 474)

1. Enzyme I, N-terminal

2. Histidine Phosphocarrier

MFQQEVTITA PNGLHTRPAA QFVKEAKGFT SEITVTSNGK SASAKSLFKL QTLGLTQGTV VTISAEGEDE QKAVEHLVKL MAELE

Show sample condition

Sample

Temperature 313 K, pH 7.0

#	Name	Concentration
1	Enzyme I, N-terminal	0.0 ~ 0.0 mM
2	Histidine Phosphocarrier	0.0 ~ 0.0 mM
3	D2O	0.0 ~ 0.0 %
4	H2O	0.0 ~ 0.0 %

Chem. Shift Complete3

Sequence coverage: 79.9 %, Completeness: 50.6 %, Completeness (bb): 61.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	50.6 % (5940 of 11739)	43.9 % (2665 of 6066)	54.9 % (2517 of 4581)	69.4 % (758 of 1092)
Backbone	61.7 % (3799 of 6156)	59.6 % (1257 of 2109)	59.9 % (1816 of 3033)	71.6 % (726 of 1014)
Sidechain	42.8 % (2803 of 6552)	35.6 % (1407 of 3957)	54.2 % (1364 of 2517)	41.0 % (32 of 78)
Aromatic	20.0 % (83 of 414)	22.2 % (46 of 207)	17.9 % (37 of 207)
Methyl	48.9 % (695 of 1422)	43.6 % (310 of 711)	54.1 % (385 of 711)

1. Enzyme I, N-terminal

2. Histidine Phosphocarrier

MFQQEVTITA PNGLHTRPAA QFVKEAKGFT SEITVTSNGK SASAKSLFKL QTLGLTQGTV VTISAEGEDE QKAVEHLVKL MAELE

Show sample condition

Sample

Temperature 313 K, pH 7.0

#	Name	Isotope labeling	Concentration
5	Enzyme I, N-terminal	[U-2H]	0.0 ~ 0.0 mM
6	Histidine Phosphocarrier	[U-2H]	0.0 ~ 0.0 mM
7	D2O		0.0 ~ 0.0 %
8	H2O		0.0 ~ 0.0 %

Chem. Shift Complete4

Sequence coverage: 55.5 %, Completeness: 46.7 %, Completeness (bb): 57.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	46.7 % (7306 of 15652)	39.9 % (3230 of 8088)	51.3 % (3132 of 6108)	64.8 % (944 of 1456)
Backbone	57.2 % (4693 of 8208)	54.4 % (1531 of 2812)	55.9 % (2259 of 4044)	66.8 % (903 of 1352)
Sidechain	39.3 % (3431 of 8736)	32.1 % (1696 of 5276)	50.5 % (1694 of 3356)	39.4 % (41 of 104)
Aromatic	18.7 % (103 of 552)	19.9 % (55 of 276)	17.4 % (48 of 276)
Methyl	45.4 % (860 of 1896)	39.8 % (377 of 948)	50.9 % (483 of 948)

1. Enzyme I, N-terminal

2. Histidine Phosphocarrier

MFQQEVTITA PNGLHTRPAA QFVKEAKGFT SEITVTSNGK SASAKSLFKL QTLGLTQGTV VTISAEGEDE QKAVEHLVKL MAELE

Show sample condition

Sample

Temperature 313 K, pH 7.0

#	Name	Isotope labeling	Concentration
5	Enzyme I, N-terminal	[U-2H]	0.0 ~ 0.0 mM
6	Histidine Phosphocarrier	[U-2H]	0.0 ~ 0.0 mM
7	D2O		0.0 ~ 0.0 %
8	H2O		0.0 ~ 0.0 %

LACS Plot; CA

Referencing offset: 0.4 ppm, Outliers: 2 Detail

LACS Plot; CB

Referencing offset: 0.4 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 1 models in PDB: 3EZA, Strand ID: A, B Detail

Release date

2000-05-01

Citation

Solution structure of the 40,000 Mr phosphoryl transfer complex between the N-terminal domain of enzyme I and HPr
GARRETT, D.S., SEOK, Y.J., PETERKOFSKY, A., GRONENBORN, A.M., CLORE, G.M.
Nat. Struct. Biol. (1999), 6, 166-173, PubMed 10048929 , DOI 10.1038/5854 , Abstract

Related entities 1. Enzyme I, N-terminal, : 9 : 10 : 52 entities Detail

Related entities 2. Histidine Phosphocarrier, : 1 : 17 : 1 : 4 : 93 entities Detail

Interaction partners 1. Enzyme I, N-terminal, : 10 : 1 interactors Detail

More details for 11 interactors identified by 5 citations

Interaction partners 2. Histidine Phosphocarrier, : 9 : 1 interactors Detail

More details for 10 interactors identified by 5 citations

Experiments performed 6 experiments Detail

1 (1) TRIPLE RESONANCE FOR ASSIGNMENT OF PROTEIN.

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

Sample #1

Temperature 313 K, pH 7.0

#	Name	Concentration
1	Enzyme I, N-terminal	0.0 ~ 0.0 mM
2	Histidine Phosphocarrier	0.0 ~ 0.0 mM
3	D2O	0.0 ~ 0.0 %
4	H2O	0.0 ~ 0.0 %

Sample #2

Temperature 313 K, pH 7.0

#	Name	Isotope labeling	Concentration
5	Enzyme I, N-terminal	[U-2H]	0.0 ~ 0.0 mM
6	Histidine Phosphocarrier	[U-2H]	0.0 ~ 0.0 mM
7	D2O		0.0 ~ 0.0 %
8	H2O		0.0 ~ 0.0 %

2 (2) QUANTITATIVE J CORRELATION FOR COUPLING CONSTANTS.

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

Sample #1

Temperature 313 K, pH 7.0

#	Name	Concentration
1	Enzyme I, N-terminal	0.0 ~ 0.0 mM
2	Histidine Phosphocarrier	0.0 ~ 0.0 mM
3	D2O	0.0 ~ 0.0 %
4	H2O	0.0 ~ 0.0 %

Sample #2

Temperature 313 K, pH 7.0

#	Name	Isotope labeling	Concentration
5	Enzyme I, N-terminal	[U-2H]	0.0 ~ 0.0 mM
6	Histidine Phosphocarrier	[U-2H]	0.0 ~ 0.0 mM
7	D2O		0.0 ~ 0.0 %
8	H2O		0.0 ~ 0.0 %

3 (3) 3D AND 4D HETERONUCLEAR SEPARATED AND FILTERED NOE E

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

Sample #1

Temperature 313 K, pH 7.0

#	Name	Concentration
1	Enzyme I, N-terminal	0.0 ~ 0.0 mM
2	Histidine Phosphocarrier	0.0 ~ 0.0 mM
3	D2O	0.0 ~ 0.0 %
4	H2O	0.0 ~ 0.0 %

Sample #2

Temperature 313 K, pH 7.0

#	Name	Isotope labeling	Concentration
5	Enzyme I, N-terminal	[U-2H]	0.0 ~ 0.0 mM
6	Histidine Phosphocarrier	[U-2H]	0.0 ~ 0.0 mM
7	D2O		0.0 ~ 0.0 %
8	H2O		0.0 ~ 0.0 %

4 (4) IPAP EXPTS FOR DIPOLAR COUPLINGS DIPOLAR COUPLINGS WERE MEASURED IN A

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

Sample #1

Temperature 313 K, pH 7.0

#	Name	Concentration
1	Enzyme I, N-terminal	0.0 ~ 0.0 mM
2	Histidine Phosphocarrier	0.0 ~ 0.0 mM
3	D2O	0.0 ~ 0.0 %
4	H2O	0.0 ~ 0.0 %

Sample #2

Temperature 313 K, pH 7.0

#	Name	Isotope labeling	Concentration
5	Enzyme I, N-terminal	[U-2H]	0.0 ~ 0.0 mM
6	Histidine Phosphocarrier	[U-2H]	0.0 ~ 0.0 mM
7	D2O		0.0 ~ 0.0 %
8	H2O		0.0 ~ 0.0 %

5 NEMATIC PHASE OF A CO SUSPENSION OF PHAGE FD (CLORE ET AL. 1998 J. AM. CHEM.

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

Sample #1

Temperature 313 K, pH 7.0

#	Name	Concentration
1	Enzyme I, N-terminal	0.0 ~ 0.0 mM
2	Histidine Phosphocarrier	0.0 ~ 0.0 mM
3	D2O	0.0 ~ 0.0 %
4	H2O	0.0 ~ 0.0 %

Sample #2

Temperature 313 K, pH 7.0

#	Name	Isotope labeling	Concentration
5	Enzyme I, N-terminal	[U-2H]	0.0 ~ 0.0 mM
6	Histidine Phosphocarrier	[U-2H]	0.0 ~ 0.0 mM
7	D2O		0.0 ~ 0.0 %
8	H2O		0.0 ~ 0.0 %

6 SOC

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

Sample #1

Temperature 313 K, pH 7.0

#	Name	Concentration
1	Enzyme I, N-terminal	0.0 ~ 0.0 mM
2	Histidine Phosphocarrier	0.0 ~ 0.0 mM
3	D2O	0.0 ~ 0.0 %
4	H2O	0.0 ~ 0.0 %

Sample #2

Temperature 313 K, pH 7.0

#	Name	Isotope labeling	Concentration
5	Enzyme I, N-terminal	[U-2H]	0.0 ~ 0.0 mM
6	Histidine Phosphocarrier	[U-2H]	0.0 ~ 0.0 mM
7	D2O		0.0 ~ 0.0 %
8	H2O		0.0 ~ 0.0 %

nullKeywords PHOSPHOTRANSFERASE, TRANSFERASE, KINASE, SUGAR TRANSPORT

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Found 1 Document (1.938 seconds)

BMRB: 4264

Sample

Sample

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Chemical shift reference

Chemical shift reference

Related entities 1. Enzyme I, N-terminal, : 9 : 10 : 52 entities Detail

Related entities 2. Histidine Phosphocarrier, : 1 : 17 : 1 : 4 : 93 entities Detail

Interaction partners 1. Enzyme I, N-terminal, : 10 : 1 interactors Detail

Interaction partners 2. Histidine Phosphocarrier, : 9 : 1 interactors Detail

Experiments performed 6 experiments Detail

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