BMRB: 4265

1H, 15N and 13C Resonance Assignments for the 22KDa LC1 Light Chain from Chlamydomonas Outer Arm Dynein

Authors

Wu, H., Maciejewski, M.W., Benashski, S.E., Mullen, G.P., King, S.M.

Assembly

Dynein light chain 1

Entity

1. Dynein light chain 1 (polymer), 199 monomers, 22287.45 Da Detail

HMAKATTIKD AIRIFEERKS VVATEAEKVE LHGMIPPIEK MDATLSTLKA CKHLALSTNN IEKISSLSGM ENLRILSLGR NLIKKIENLD AVADTLEELW ISYNQIASLS GIEKLVNLRV LYMSNNKITN WGEIDKLAAL DKLEDLLLAG NPLYNDYKEN NATSEYRIEV VKRLPNLKKL DGMPVDVDER EQANVARGG

Formula weight

22287.45 Da

Source organism

Chlamydomonas reinhardtii

Exptl. method

NMR

Refine. method

simulated annealing, torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.5 %, Completeness: 77.3 %, Completeness (bb): 97.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	77.3 % (1805 of 2335)	73.5 % (895 of 1217)	78.1 % (706 of 904)	95.3 % (204 of 214)
Backbone	97.7 % (1157 of 1184)	97.8 % (393 of 402)	97.6 % (574 of 588)	97.9 % (190 of 194)
Sidechain	62.3 % (835 of 1341)	61.6 % (502 of 815)	63.0 % (319 of 506)	70.0 % (14 of 20)
Aromatic	7.0 % (6 of 86)	9.3 % (4 of 43)	0.0 % (0 of 41)	100.0 % (2 of 2)
Methyl	67.9 % (182 of 268)	79.1 % (106 of 134)	56.7 % (76 of 134)

1. Dynein light chain 1

HMAKATTIKD AIRIFEERKS VVATEAEKVE LHGMIPPIEK MDATLSTLKA CKHLALSTNN IEKISSLSGM ENLRILSLGR NLIKKIENLD AVADTLEELW ISYNQIASLS GIEKLVNLRV LYMSNNKITN WGEIDKLAAL DKLEDLLLAG NPLYNDYKEN NATSEYRIEV VKRLPNLKKL DGMPVDVDER EQANVARGG

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Sample

Temperature 298 (±1) K, pH 6.76 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Dynein light chain 1	[U-15N]		1.0 mM

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LACS Plot; CA

Referencing offset: -0.57 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	H2O	protons	4.772 ppm	internal	direct	0.0

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LACS Plot; CB

Referencing offset: -0.57 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	H2O	protons	4.772 ppm	internal	direct	0.0

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LACS Plot; HA

Referencing offset: -0.1 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	H2O	protons	4.772 ppm	internal	direct	0.0

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LACS Plot; CO

Referencing offset: 0.22 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	H2O	protons	4.772 ppm	internal	direct	0.0

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Protein Blocks Logo

Calculated from 17 models in PDB: 1DS9, Strand ID: A Detail

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Release date

1999-09-26

Citation

Assessment of inner dynein arm structure and possible function in ciliary and flagellar axonemes
Wu, H., Maciejewski, M.W., Benashski, S.E., Mullen, G.P., King, S.M.
Cell Motil. Cytoskeleton (1999), 43, 167-177, PubMed 10379841 , DOI 10.1002/(SICI)1097-0169(1999)43:2<167::AID-CM8>3.0.CO;2-Q , Abstract

Related entities 1. Dynein light chain 1, : 1 : 1 : 3 : 31 entities Detail

Experiments performed 14 experiments Detail