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The Solution Structure of a Superpotent B-chain-shortened Single-replacement Insulin Analogue
Authors
Kurapkat, G., Siedentop, M., Gattner, H., Hagelstein, M., Brandenburg, D., Grotzinger, J., Wollmer, A.
Assembly
[D-AlaB26]destetra(B27-B30)insulin-B26-amide
Entity
1. [D-AlaB26]destetra(B27-B30)insulin-B26-amide (polymer), 47 monomers, 5276.010 × 2 Da Detail

GIVEQCCTSI CSLYQLENYC NFVNQHLCGS HLVEALYLVC GERGFFA


Total weight
10552.02 Da
Max. entity weight
5276.01 Da
Source organism
Homo sapiens
Exptl. method
NMR
Refine. method
DISTANCE GEOMETRY
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 87.9 %, Completeness (bb): 96.8 % Detail

Polymer type: polypeptide(L)

Total1H
All87.9 % (239 of 272)87.9 % (239 of 272)
Backbone96.8 % (91 of 94)96.8 % (91 of 94)
Sidechain83.1 % (148 of 178)83.1 % (148 of 178)
Aromatic48.4 % (15 of 31)48.4 % (15 of 31)
Methyl96.3 % (26 of 27)96.3 % (26 of 27)

1. [D-AlaB26]destetra(B27-B30)insulin-B26-amide

GIVEQCCTSI CSLYQLENYC NFVNQHLCGS HLVEALYLVC GERGFFA

Sample

Temperature 298 (±1) K


#NameIsotope labelingTypeConcentration
1[D-AlaB26]destetra(B27-B30)insulin-B26-amide4 mM

Protein Blocks Logo
Calculated from 1 models in PDB: 1BZV, Strand ID: A, B Detail


Release date
1999-10-04
Citation
The solution structure of a superpotent B-chain-shortened single-replacement insulin analogue
Kurapkat, G., Siedentop, M., Gattner, H., Hagelstein, M., Brandenburg, D., Grotzinger, J., Wollmer, A.
Protein Sci. (1999), 8, 499-508, PubMed 10091652 , DOI 10.1110/ps.8.3.499 ,
Related entities 1. [D-AlaB26]destetra(B27-B30)insulin-B26-amide, : 3 entities Detail
Keywords active conformation, CD spectroscopy, insulin, NMR spectroscopy, receptor_binding, structure, superpotency