BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	91.3 % (1098 of 1203)	92.4 % (581 of 629)	89.4 % (414 of 463)	92.8 % (103 of 111)
Backbone	94.1 % (593 of 630)	93.6 % (206 of 220)	95.1 % (290 of 305)	92.4 % (97 of 105)
Sidechain	89.5 % (598 of 668)	91.9 % (376 of 409)	85.4 % (216 of 253)	100.0 % (6 of 6)
Aromatic	47.9 % (23 of 48)	87.5 % (21 of 24)	8.3 % (2 of 24)
Methyl	99.2 % (121 of 122)	98.4 % (60 of 61)	100.0 % (61 of 61)

1. resolvase

MRLFGYARVS TSQQSLDIQV RALKDAGVKA NRIFTDKASG SSSDRKGLDL LRMKVEEGDV ILVKKLDRLG RDTADMIQLI KEFDAQGVSI RFIDDGISTD GEMGK

Show sample condition

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	resolvase	[U-15N]	2.0 mM
2	D2O		10 %
3	H2O		90 %

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	resolvase	[U-13C; U-15N]		2.0 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.45 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.45 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.17 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.1 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 1 models in PDB: 1GHT, Strand ID: A Detail

Release date

1999-10-13

Citation

1H, 15N and 13C Chemical Shift Assignments for the Catalytic Core of Resolvase
Pan, B., Mullen, G.P.
J. Biomol. NMR (1999), 13, 307-308, PubMed , DOI:

Related entities 1. gamma delta resolvase catalytic core, : 1 : 5 : 4 : 42 entities Detail

Experiments performed 9 experiments Detail

1 HNCA

Instrument

#1 Model Varian na (500 MHz)

#2 Model Varian na (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	resolvase	[U-15N]	2.0 mM
2	D2O		10 %
3	H2O		90 %

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	resolvase	[U-13C; U-15N]		2.0 mM

2 HNCOCA

Instrument

#1 Model Varian na (500 MHz)

#2 Model Varian na (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	resolvase	[U-15N]	2.0 mM
2	D2O		10 %
3	H2O		90 %

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	resolvase	[U-13C; U-15N]		2.0 mM

3 HNCACB

Instrument

#1 Model Varian na (500 MHz)

#2 Model Varian na (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	resolvase	[U-15N]	2.0 mM
2	D2O		10 %
3	H2O		90 %

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	resolvase	[U-13C; U-15N]		2.0 mM

4 CBCACONH

Instrument

#1 Model Varian na (500 MHz)

#2 Model Varian na (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	resolvase	[U-15N]	2.0 mM
2	D2O		10 %
3	H2O		90 %

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	resolvase	[U-13C; U-15N]		2.0 mM

5 HNCO,

Instrument

#1 Model Varian na (500 MHz)

#2 Model Varian na (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	resolvase	[U-15N]	2.0 mM
2	D2O		10 %
3	H2O		90 %

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	resolvase	[U-13C; U-15N]		2.0 mM

6 CCONH

Instrument

#1 Model Varian na (500 MHz)

#2 Model Varian na (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	resolvase	[U-15N]	2.0 mM
2	D2O		10 %
3	H2O		90 %

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	resolvase	[U-13C; U-15N]		2.0 mM

7 HCCONH

Instrument

#1 Model Varian na (500 MHz)

#2 Model Varian na (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	resolvase	[U-15N]	2.0 mM
2	D2O		10 %
3	H2O		90 %

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	resolvase	[U-13C; U-15N]		2.0 mM

8 HCCH-TOCSY

Instrument

#1 Model Varian na (500 MHz)

#2 Model Varian na (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	resolvase	[U-15N]	2.0 mM
2	D2O		10 %
3	H2O		90 %

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	resolvase	[U-13C; U-15N]		2.0 mM

9 1H-NOESY

Instrument

#1 Model Varian na (500 MHz)

#2 Model Varian na (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	resolvase	[U-15N]	2.0 mM
2	D2O		10 %
3	H2O		90 %

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	resolvase	[U-13C; U-15N]		2.0 mM

nullKeywords resolvase, recombination, DNA, Protein, NMR, Structure

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BMRB: 4269

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. gamma delta resolvase catalytic core, : 1 : 5 : 4 : 42 entities Detail

Experiments performed 9 experiments Detail

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