BMRBj - BMREntryMaster

Found 1 Document (1.517 seconds)

BMRB: 4281

1Z0R

Backbone and Side Chain 1H, 13C, and 15N Chemical Shift Assignments for AbrBN

Authors

Bobay, B.G., Andreeva, A., Mueller, G.A., Cavanagh, J., Murzin, A.G.

Assembly

AbrBN

Entity

1. AbrBN (polymer), 53 monomers, 6100.197 × 2 Da Detail

MKSTGIVRKV DELGRVVIPI ELRRTLGIAE KDALEIYVDD EKIILKKYKP NMT

Total weight

12200.394 Da

Max. entity weight

6100.197 Da

Source organism

Bacillus subtilis

Exptl. method

NMR

Refine. method

distance geometry, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.1 %, Completeness: 94.5 %, Completeness (bb): 96.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	94.5 % (613 of 649)	95.0 % (325 of 342)	93.3 % (238 of 255)	96.2 % (50 of 52)
Backbone	96.5 % (303 of 314)	97.2 % (104 of 107)	96.2 % (150 of 156)	96.1 % (49 of 51)
Sidechain	93.2 % (359 of 385)	94.0 % (221 of 235)	91.9 % (137 of 149)	100.0 % (1 of 1)
Aromatic	0.0 % (0 of 16)	0.0 % (0 of 8)	0.0 % (0 of 8)
Methyl	100.0 % (78 of 78)	100.0 % (39 of 39)	100.0 % (39 of 39)

1. AbrB

MKSTGIVRKV DELGRVVIPI ELRRTLGIAE KDALEIYVDD EKIILKKYKP NMT

Show sample condition

Sample #1

Temperature 305 (±1) K, pH 5.8 (±0.1), Details pH 5.8, 15 mM KCl, 20 mM K-PO, 1mM DTT, .02% NaN3 All experiments run at 305 K. Samples were in 95%H2O/5%D2O.

#	Name	Isotope labeling	Concentration
1	AbrB	[U-99% 13C; U-99% 15N]	1.5 ~ 2.5 mM
2	KCl		15 mM
3	K-PO		10 mM
4	DTT		1 mM
5	NaN3		0.02 %
6	D20		5 %
7	H2O		95 %

Sample #2

Temperature 305 (±1) K, pH 5.8 (±0.1), Details pH 5.8, 15 mM KCl, 20 mM K-PO, 1mM DTT, .02% NaN3 All experiments run at 305 K. Samples were in 99% d2o buffers.

#	Name	Isotope labeling	Concentration
8	AbrB	[U-99% 13C; U-99% 15N]	1.5 ~ 2.5 mM
9	KCl		15 mM
10	K-PO		10 mM
11	DTT		1 mM
12	NaN3		0.02 %
13	D20		5 %
14	H2O		95 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	H2O	protons	internal	direct	1.0
¹³C	TMS	methyl protons	null	indirect	0.2514495
¹⁵N	TMS	methyl protons	null	indirect	0.1013291

Referencing offset: 2.57 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	H2O	protons	internal	direct	1.0
¹³C	TMS	methyl protons	null	indirect	0.2514495
¹⁵N	TMS	methyl protons	null	indirect	0.1013291

Referencing offset: 2.57 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	H2O	protons	internal	direct	1.0
¹³C	TMS	methyl protons	null	indirect	0.2514495
¹⁵N	TMS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	H2O	protons	internal	direct	1.0
¹³C	TMS	methyl protons	null	indirect	0.2514495
¹⁵N	TMS	methyl protons	null	indirect	0.1013291

Referencing offset: -1.08 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 7 models in PDB: 1Z0R, Strand ID: A, B Detail

Release date

2001-01-10

Citation

Revised structure of the AbrB N-terminal domain unifies a diverse superfamily of putative DNA-binding proteins
Bobay, B.G., Andreeva, A., Mueller, G.A., Cavanagh, J., Murzin, A.G.
FEBS Lett. (2005), 579, 5669-5674, PubMed 16223496 , DOI 10.1016/j.febslet.2005.09.045 , Abstract

Related entities 1. AbrBN, : 1 : 3 : 2 : 7 entities Detail

Interaction partners 1. AbrBN, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 12 experiments Detail

1 1H-15N NOESY (120mS and 150mS mixing time)

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Temperature 305 (±1) K, pH 5.8 (±0.1), Details pH 5.8, 15 mM KCl, 20 mM K-PO, 1mM DTT, .02% NaN3 All experiments run at 305 K. Samples were in 95%H2O/5%D2O.

#	Name	Isotope labeling	Concentration
1	AbrB	[U-99% 13C; U-99% 15N]	1.5 ~ 2.5 mM
2	KCl		15 mM
3	K-PO		10 mM
4	DTT		1 mM
5	NaN3		0.02 %
6	D20		5 %
7	H2O		95 %

Sample #2

Temperature 305 (±1) K, pH 5.8 (±0.1), Details pH 5.8, 15 mM KCl, 20 mM K-PO, 1mM DTT, .02% NaN3 All experiments run at 305 K. Samples were in 99% d2o buffers.

#	Name	Isotope labeling	Concentration
8	AbrB	[U-99% 13C; U-99% 15N]	1.5 ~ 2.5 mM
9	KCl		15 mM
10	K-PO		10 mM
11	DTT		1 mM
12	NaN3		0.02 %
13	D20		5 %
14	H2O		95 %

2 1H-13C COESY (120mS and 120mS folded mixing time)

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Temperature 305 (±1) K, pH 5.8 (±0.1), Details pH 5.8, 15 mM KCl, 20 mM K-PO, 1mM DTT, .02% NaN3 All experiments run at 305 K. Samples were in 95%H2O/5%D2O.

#	Name	Isotope labeling	Concentration
1	AbrB	[U-99% 13C; U-99% 15N]	1.5 ~ 2.5 mM
2	KCl		15 mM
3	K-PO		10 mM
4	DTT		1 mM
5	NaN3		0.02 %
6	D20		5 %
7	H2O		95 %

Sample #2

Temperature 305 (±1) K, pH 5.8 (±0.1), Details pH 5.8, 15 mM KCl, 20 mM K-PO, 1mM DTT, .02% NaN3 All experiments run at 305 K. Samples were in 99% d2o buffers.

#	Name	Isotope labeling	Concentration
8	AbrB	[U-99% 13C; U-99% 15N]	1.5 ~ 2.5 mM
9	KCl		15 mM
10	K-PO		10 mM
11	DTT		1 mM
12	NaN3		0.02 %
13	D20		5 %
14	H2O		95 %

3 3-D HNCA

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Temperature 305 (±1) K, pH 5.8 (±0.1), Details pH 5.8, 15 mM KCl, 20 mM K-PO, 1mM DTT, .02% NaN3 All experiments run at 305 K. Samples were in 95%H2O/5%D2O.

#	Name	Isotope labeling	Concentration
1	AbrB	[U-99% 13C; U-99% 15N]	1.5 ~ 2.5 mM
2	KCl		15 mM
3	K-PO		10 mM
4	DTT		1 mM
5	NaN3		0.02 %
6	D20		5 %
7	H2O		95 %

Sample #2

Temperature 305 (±1) K, pH 5.8 (±0.1), Details pH 5.8, 15 mM KCl, 20 mM K-PO, 1mM DTT, .02% NaN3 All experiments run at 305 K. Samples were in 99% d2o buffers.

#	Name	Isotope labeling	Concentration
8	AbrB	[U-99% 13C; U-99% 15N]	1.5 ~ 2.5 mM
9	KCl		15 mM
10	K-PO		10 mM
11	DTT		1 mM
12	NaN3		0.02 %
13	D20		5 %
14	H2O		95 %

4 3-D HN(CO)CA

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Temperature 305 (±1) K, pH 5.8 (±0.1), Details pH 5.8, 15 mM KCl, 20 mM K-PO, 1mM DTT, .02% NaN3 All experiments run at 305 K. Samples were in 95%H2O/5%D2O.

#	Name	Isotope labeling	Concentration
1	AbrB	[U-99% 13C; U-99% 15N]	1.5 ~ 2.5 mM
2	KCl		15 mM
3	K-PO		10 mM
4	DTT		1 mM
5	NaN3		0.02 %
6	D20		5 %
7	H2O		95 %

Sample #2

Temperature 305 (±1) K, pH 5.8 (±0.1), Details pH 5.8, 15 mM KCl, 20 mM K-PO, 1mM DTT, .02% NaN3 All experiments run at 305 K. Samples were in 99% d2o buffers.

#	Name	Isotope labeling	Concentration
8	AbrB	[U-99% 13C; U-99% 15N]	1.5 ~ 2.5 mM
9	KCl		15 mM
10	K-PO		10 mM
11	DTT		1 mM
12	NaN3		0.02 %
13	D20		5 %
14	H2O		95 %

5 3-D HNCO

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Temperature 305 (±1) K, pH 5.8 (±0.1), Details pH 5.8, 15 mM KCl, 20 mM K-PO, 1mM DTT, .02% NaN3 All experiments run at 305 K. Samples were in 95%H2O/5%D2O.

#	Name	Isotope labeling	Concentration
1	AbrB	[U-99% 13C; U-99% 15N]	1.5 ~ 2.5 mM
2	KCl		15 mM
3	K-PO		10 mM
4	DTT		1 mM
5	NaN3		0.02 %
6	D20		5 %
7	H2O		95 %

Sample #2

Temperature 305 (±1) K, pH 5.8 (±0.1), Details pH 5.8, 15 mM KCl, 20 mM K-PO, 1mM DTT, .02% NaN3 All experiments run at 305 K. Samples were in 99% d2o buffers.

#	Name	Isotope labeling	Concentration
8	AbrB	[U-99% 13C; U-99% 15N]	1.5 ~ 2.5 mM
9	KCl		15 mM
10	K-PO		10 mM
11	DTT		1 mM
12	NaN3		0.02 %
13	D20		5 %
14	H2O		95 %

6 3-D HN(CA)CO

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Temperature 305 (±1) K, pH 5.8 (±0.1), Details pH 5.8, 15 mM KCl, 20 mM K-PO, 1mM DTT, .02% NaN3 All experiments run at 305 K. Samples were in 95%H2O/5%D2O.

#	Name	Isotope labeling	Concentration
1	AbrB	[U-99% 13C; U-99% 15N]	1.5 ~ 2.5 mM
2	KCl		15 mM
3	K-PO		10 mM
4	DTT		1 mM
5	NaN3		0.02 %
6	D20		5 %
7	H2O		95 %

Sample #2

Temperature 305 (±1) K, pH 5.8 (±0.1), Details pH 5.8, 15 mM KCl, 20 mM K-PO, 1mM DTT, .02% NaN3 All experiments run at 305 K. Samples were in 99% d2o buffers.

#	Name	Isotope labeling	Concentration
8	AbrB	[U-99% 13C; U-99% 15N]	1.5 ~ 2.5 mM
9	KCl		15 mM
10	K-PO		10 mM
11	DTT		1 mM
12	NaN3		0.02 %
13	D20		5 %
14	H2O		95 %

7 3-D CBCA(CO)NH

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Temperature 305 (±1) K, pH 5.8 (±0.1), Details pH 5.8, 15 mM KCl, 20 mM K-PO, 1mM DTT, .02% NaN3 All experiments run at 305 K. Samples were in 95%H2O/5%D2O.

#	Name	Isotope labeling	Concentration
1	AbrB	[U-99% 13C; U-99% 15N]	1.5 ~ 2.5 mM
2	KCl		15 mM
3	K-PO		10 mM
4	DTT		1 mM
5	NaN3		0.02 %
6	D20		5 %
7	H2O		95 %

Sample #2

Temperature 305 (±1) K, pH 5.8 (±0.1), Details pH 5.8, 15 mM KCl, 20 mM K-PO, 1mM DTT, .02% NaN3 All experiments run at 305 K. Samples were in 99% d2o buffers.

#	Name	Isotope labeling	Concentration
8	AbrB	[U-99% 13C; U-99% 15N]	1.5 ~ 2.5 mM
9	KCl		15 mM
10	K-PO		10 mM
11	DTT		1 mM
12	NaN3		0.02 %
13	D20		5 %
14	H2O		95 %

8 3-D HNCACB

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Temperature 305 (±1) K, pH 5.8 (±0.1), Details pH 5.8, 15 mM KCl, 20 mM K-PO, 1mM DTT, .02% NaN3 All experiments run at 305 K. Samples were in 95%H2O/5%D2O.

#	Name	Isotope labeling	Concentration
1	AbrB	[U-99% 13C; U-99% 15N]	1.5 ~ 2.5 mM
2	KCl		15 mM
3	K-PO		10 mM
4	DTT		1 mM
5	NaN3		0.02 %
6	D20		5 %
7	H2O		95 %

Sample #2

Temperature 305 (±1) K, pH 5.8 (±0.1), Details pH 5.8, 15 mM KCl, 20 mM K-PO, 1mM DTT, .02% NaN3 All experiments run at 305 K. Samples were in 99% d2o buffers.

#	Name	Isotope labeling	Concentration
8	AbrB	[U-99% 13C; U-99% 15N]	1.5 ~ 2.5 mM
9	KCl		15 mM
10	K-PO		10 mM
11	DTT		1 mM
12	NaN3		0.02 %
13	D20		5 %
14	H2O		95 %

9 D2O exchange via 2-D 1H-15N HSQC

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Temperature 305 (±1) K, pH 5.8 (±0.1), Details pH 5.8, 15 mM KCl, 20 mM K-PO, 1mM DTT, .02% NaN3 All experiments run at 305 K. Samples were in 95%H2O/5%D2O.

#	Name	Isotope labeling	Concentration
1	AbrB	[U-99% 13C; U-99% 15N]	1.5 ~ 2.5 mM
2	KCl		15 mM
3	K-PO		10 mM
4	DTT		1 mM
5	NaN3		0.02 %
6	D20		5 %
7	H2O		95 %

Sample #2

Temperature 305 (±1) K, pH 5.8 (±0.1), Details pH 5.8, 15 mM KCl, 20 mM K-PO, 1mM DTT, .02% NaN3 All experiments run at 305 K. Samples were in 99% d2o buffers.

#	Name	Isotope labeling	Concentration
8	AbrB	[U-99% 13C; U-99% 15N]	1.5 ~ 2.5 mM
9	KCl		15 mM
10	K-PO		10 mM
11	DTT		1 mM
12	NaN3		0.02 %
13	D20		5 %
14	H2O		95 %

10 CC-TOCSY-(CO)NH

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Temperature 305 (±1) K, pH 5.8 (±0.1), Details pH 5.8, 15 mM KCl, 20 mM K-PO, 1mM DTT, .02% NaN3 All experiments run at 305 K. Samples were in 95%H2O/5%D2O.

#	Name	Isotope labeling	Concentration
1	AbrB	[U-99% 13C; U-99% 15N]	1.5 ~ 2.5 mM
2	KCl		15 mM
3	K-PO		10 mM
4	DTT		1 mM
5	NaN3		0.02 %
6	D20		5 %
7	H2O		95 %

Sample #2

Temperature 305 (±1) K, pH 5.8 (±0.1), Details pH 5.8, 15 mM KCl, 20 mM K-PO, 1mM DTT, .02% NaN3 All experiments run at 305 K. Samples were in 99% d2o buffers.

#	Name	Isotope labeling	Concentration
8	AbrB	[U-99% 13C; U-99% 15N]	1.5 ~ 2.5 mM
9	KCl		15 mM
10	K-PO		10 mM
11	DTT		1 mM
12	NaN3		0.02 %
13	D20		5 %
14	H2O		95 %

11 H(CC-TOCSY-CO)NH

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Temperature 305 (±1) K, pH 5.8 (±0.1), Details pH 5.8, 15 mM KCl, 20 mM K-PO, 1mM DTT, .02% NaN3 All experiments run at 305 K. Samples were in 95%H2O/5%D2O.

#	Name	Isotope labeling	Concentration
1	AbrB	[U-99% 13C; U-99% 15N]	1.5 ~ 2.5 mM
2	KCl		15 mM
3	K-PO		10 mM
4	DTT		1 mM
5	NaN3		0.02 %
6	D20		5 %
7	H2O		95 %

Sample #2

Temperature 305 (±1) K, pH 5.8 (±0.1), Details pH 5.8, 15 mM KCl, 20 mM K-PO, 1mM DTT, .02% NaN3 All experiments run at 305 K. Samples were in 99% d2o buffers.

#	Name	Isotope labeling	Concentration
8	AbrB	[U-99% 13C; U-99% 15N]	1.5 ~ 2.5 mM
9	KCl		15 mM
10	K-PO		10 mM
11	DTT		1 mM
12	NaN3		0.02 %
13	D20		5 %
14	H2O		95 %

12 HCCH-TOCSY

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Temperature 305 (±1) K, pH 5.8 (±0.1), Details pH 5.8, 15 mM KCl, 20 mM K-PO, 1mM DTT, .02% NaN3 All experiments run at 305 K. Samples were in 95%H2O/5%D2O.

#	Name	Isotope labeling	Concentration
1	AbrB	[U-99% 13C; U-99% 15N]	1.5 ~ 2.5 mM
2	KCl		15 mM
3	K-PO		10 mM
4	DTT		1 mM
5	NaN3		0.02 %
6	D20		5 %
7	H2O		95 %

Sample #2

Temperature 305 (±1) K, pH 5.8 (±0.1), Details pH 5.8, 15 mM KCl, 20 mM K-PO, 1mM DTT, .02% NaN3 All experiments run at 305 K. Samples were in 99% d2o buffers.

#	Name	Isotope labeling	Concentration
8	AbrB	[U-99% 13C; U-99% 15N]	1.5 ~ 2.5 mM
9	KCl		15 mM
10	K-PO		10 mM
11	DTT		1 mM
12	NaN3		0.02 %
13	D20		5 %
14	H2O		95 %

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Found 1 Document (1.517 seconds)

BMRB: 4281

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. AbrBN, : 1 : 3 : 2 : 7 entities Detail

Interaction partners 1. AbrBN, : 1 interactors Detail

Experiments performed 12 experiments Detail

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