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Assignment of 1H, 13C and 15N Signals of the DNase Domain of Colicin E9
Authors
Whittaker, S., Boetzel, R., MacDonald, C., Lian, L., James, R., Kleanthous, C., Moore, G.
Assembly
DNase domain of colicin E9
Entity
1. DNase domain of colicin E9 (polymer), 134 monomers, 15087.75 × 2 Da Detail

MESKRNKPGK ATGKGKPVGD KWLDDAGKDS GAPIPDRIAD KLRDKEFKSF DDFRKAVWEE VSKDPELSKN LNPSNKSSVS KGYSPFTPKN QQVGGRKVYE LHHDKPISQG GEVYDMDNIR VTTPKRHIDI HRGK


Total weight
30175.5 Da
Max. entity weight
15087.75 Da
Source organism
Escherichia coli
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete1
Sequence coverage: 97.0 %, Completeness: 64.4 %, Completeness (bb): 92.9 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All64.4 % (1026 of 1592)59.8 % (506 of 846)65.5 % (395 of 603)87.4 % (125 of 143)
Backbone92.9 % (717 of 772)91.1 % (235 of 258)93.3 % (364 of 390)95.2 % (118 of 124)
Sidechain45.0 % (424 of 942)46.1 % (271 of 588)43.6 % (146 of 335)36.8 % (7 of 19)
Aromatic 5.8 % (6 of 104) 7.7 % (4 of 52) 0.0 % (0 of 50)100.0 % (2 of 2)
Methyl27.7 % (26 of 94)38.3 % (18 of 47)17.0 % (8 of 47)

1. DNase domain of colicin E9

MESKRNKPGK ATGKGKPVGD KWLDDAGKDS GAPIPDRIAD KLRDKEFKSF DDFRKAVWEE VSKDPELSKN LNPSNKSSVS KGYSPFTPKN QQVGGRKVYE LHHDKPISQG GEVYDMDNIR VTTPKRHIDI HRGK

Sample #1

Temperature 293 (±1) K, pH 6.2 (±0.2)


#NameIsotope labelingTypeConcentration
1DNase domain of colicin E9[U-99% 13C; U-99% 15N]2.0 mM
2sodium phosphate25.0 mM
3H2090 %
4D2010 %
5sodium azide0.01 %
Sample #2

Temperature 293 (±1) K, pH 6.2 (±0.2)


#NameIsotope labelingTypeConcentration
6DNase domain of colicin E9[U-99% 15N]1.5 mM
7sodium phosphate50 mM
8H2090 %
9D2010 %
10sodium azide0.01 %

Chem. Shift Complete2
Sequence coverage: 47.8 %, Completeness: 45.1 %, Completeness (bb): 66.8 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All45.1 % (1437 of 3184)40.8 % (691 of 1692)46.6 % (562 of 1206)64.3 % (184 of 286)
Backbone66.8 % (1031 of 1544)64.7 % (334 of 516)66.8 % (521 of 780)71.0 % (176 of 248)
Sidechain30.4 % (572 of 1884)30.4 % (357 of 1176)30.9 % (207 of 670)21.1 % (8 of 38)
Aromatic 3.8 % (8 of 208) 4.8 % (5 of 104) 0.0 % (0 of 100)75.0 % (3 of 4)
Methyl21.8 % (41 of 188)29.8 % (28 of 94)13.8 % (13 of 94)

1. DNase domain of colicin E9

MESKRNKPGK ATGKGKPVGD KWLDDAGKDS GAPIPDRIAD KLRDKEFKSF DDFRKAVWEE VSKDPELSKN LNPSNKSSVS KGYSPFTPKN QQVGGRKVYE LHHDKPISQG GEVYDMDNIR VTTPKRHIDI HRGK

Sample #1

Temperature 293 (±1) K, pH 6.2 (±0.2)


#NameIsotope labelingTypeConcentration
1DNase domain of colicin E9[U-99% 13C; U-99% 15N]2.0 mM
2sodium phosphate25.0 mM
3H2090 %
4D2010 %
5sodium azide0.01 %
Sample #2

Temperature 293 (±1) K, pH 6.2 (±0.2)


#NameIsotope labelingTypeConcentration
6DNase domain of colicin E9[U-99% 15N]1.5 mM
7sodium phosphate50 mM
8H2090 %
9D2010 %
10sodium azide0.01 %

LACS Plot; CA
Referencing offset: 0.85 ppm, Outliers: 3 Detail
LACS Plot; CB
Referencing offset: 0.85 ppm, Outliers: 3 Detail
LACS Plot; HA
Referencing offset: -0.03 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: -0.05 ppm, Outliers: 2 Detail
Release date
1999-03-08
Citation 1
Assignment of 1H, 13C and 15N signals of the DNase domain of colicin E9
Whittaker, S., Boetzel, R., MacDonald, C., Lian, L., James, R., Kleanthous, C., Moore, G.
J. Biomol. NMR (1999), 14, 201-202, PubMed 10610142 , DOI: ,
Citation 2
NMRPipe: a multidimensional spectral processing system based on UNIX pipes
Delaglio, F., Grzesiek, S., Vuister, G.W., Zhu, G., Pfeifer, J., Bax, A.
J. Biomol. NMR (1995), 6, 277-293, PubMed 8520220 , DOI 10.1007/bf00197809 ,
Citation 3
The program XEASY for computer-supported NMR spectral analysis of biological macromolecules
J. Biomol. NMR (1995), 6, 1-10, PubMed 22911575 , DOI 10.1007/BF00417486 ,
Citation 4
Complete nucleotide sequence of the colicin E9 (cei) gene
Eaton, T., James, R.
Nucleic Acids Res. (1989), 17, 1761-1761, PubMed 2646600 , DOI 10.1093/nar/17.4.1761 ,
Related entities 1. DNase domain of colicin E9, : 1 : 10 : 10 : 28 entities Detail
Interaction partners 1. DNase domain of colicin E9, : 4 interactors Detail
Experiments performed 9 experiments Detail
Keywords chemical exchange, colicin, EXSY