BMRB: 4321

1H, 13C, and 15N Chemical Shift Assignments for the Catalytic Domain of Tetrahymena GCN5 Histone Acetyltransferase in Complex with CoA

Authors

Lin, Y., Wagner, G.

Assembly

p55 monomer in complex with CoA

Entity

1. Tetrahymena histone acetyltransferase (polymer, Thiol state: all free), 166 monomers, 19698.71 Da Detail

MKGLLDFDIL TNDGTHRNMK LLIDLKNIFS RQLPKMPKEY IVKLVFDRHH ESMVILKNKQ KVIGGICFRQ YKPQRFAEVA FLAVTANEQV RGYGTRLMNK FKDHMQKQNI EYLLTYADNF AIGYFKKQGF TKEHRMPQEK WKGYIKDYDG GTLMECYIHP YVDYGN

2. COENZYME A (non-polymer), 767.534 Da

Total weight

20466.244 Da

Max. entity weight

19698.71 Da

Source organism

Tetrahymena thermophila

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.8 %, Completeness: 88.5 %, Completeness (bb): 96.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	88.5 % (1861 of 2103)	92.1 % (1017 of 1104)	82.7 % (671 of 811)	92.0 % (173 of 188)
Backbone	96.3 % (938 of 974)	96.9 % (317 of 327)	96.1 % (467 of 486)	95.7 % (154 of 161)
Sidechain	83.5 % (1071 of 1283)	90.1 % (700 of 777)	73.5 % (352 of 479)	70.4 % (19 of 27)
Aromatic	41.5 % (93 of 224)	75.9 % (85 of 112)	6.3 % (7 of 111)	100.0 % (1 of 1)
Methyl	100.0 % (158 of 158)	100.0 % (79 of 79)	100.0 % (79 of 79)

1. Tetrahymena histone acetyltransferase

MKGLLDFDIL TNDGTHRNMK LLIDLKNIFS RQLPKMPKEY IVKLVFDRHH ESMVILKNKQ KVIGGICFRQ YKPQRFAEVA FLAVTANEQV RGYGTRLMNK FKDHMQKQNI EYLLTYADNF AIGYFKKQGF TKEHRMPQEK WKGYIKDYDG GTLMECYIHP YVDYGN

Show sample condition

Sample

Temperature 300 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Tetrahymena histone acetyltransferase			0.4 mM
2	coenzyme A			0.8 mM
3	sodium phosphate			50 mM
4	NaCl			100 mM
5	DTT			0.1 mM
6	NaN3			0.1 mM
7	EDTA			0.1 mM

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LACS Plot; CA

Referencing offset: -0.1 ppm, Outliers: 4 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.1 ppm, Outliers: 4 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.04 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 1.21 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Release date

2000-12-13

Citation

Efficient side-chain and backbone assignment in large proteins: application to tGCN5
Lin, Y., Wagner, G.
J. Biomol. NMR (1999), 15, 227-239, PubMed 10677826 , DOI: , Abstract

Related entities 1. Tetrahymena histone acetyltransferase, : 1 : 8 : 23 entities Detail

Experiments performed 1 experiments Detail