1H, 13C, and 15N Chemical Shift Assignments for the Catalytic Domain of Tetrahymena GCN5 Histone Acetyltransferase in Complex with CoA
MKGLLDFDIL TNDGTHRNMK LLIDLKNIFS RQLPKMPKEY IVKLVFDRHH ESMVILKNKQ KVIGGICFRQ YKPQRFAEVA FLAVTANEQV RGYGTRLMNK FKDHMQKQNI EYLLTYADNF AIGYFKKQGF TKEHRMPQEK WKGYIKDYDG GTLMECYIHP YVDYGN
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 88.5 % (1861 of 2103) | 92.1 % (1017 of 1104) | 82.7 % (671 of 811) | 92.0 % (173 of 188) |
Backbone | 96.3 % (938 of 974) | 96.9 % (317 of 327) | 96.1 % (467 of 486) | 95.7 % (154 of 161) |
Sidechain | 83.5 % (1071 of 1283) | 90.1 % (700 of 777) | 73.5 % (352 of 479) | 70.4 % (19 of 27) |
Aromatic | 41.5 % (93 of 224) | 75.9 % (85 of 112) | 6.3 % (7 of 111) | 100.0 % (1 of 1) |
Methyl | 100.0 % (158 of 158) | 100.0 % (79 of 79) | 100.0 % (79 of 79) |
1. Tetrahymena histone acetyltransferase
MKGLLDFDIL TNDGTHRNMK LLIDLKNIFS RQLPKMPKEY IVKLVFDRHH ESMVILKNKQ KVIGGICFRQ YKPQRFAEVA FLAVTANEQV RGYGTRLMNK FKDHMQKQNI EYLLTYADNF AIGYFKKQGF TKEHRMPQEK WKGYIKDYDG GTLMECYIHP YVDYGNTemperature 300 (±1) K, pH 6.5 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Tetrahymena histone acetyltransferase | 0.4 mM | ||
2 | coenzyme A | 0.8 mM | ||
3 | sodium phosphate | 50 mM | ||
4 | NaCl | 100 mM | ||
5 | DTT | 0.1 mM | ||
6 | NaN3 | 0.1 mM | ||
7 | EDTA | 0.1 mM |