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1H, 13C, and 15N Chemical Shift Assignments for the Catalytic Domain of Tetrahymena GCN5 Histone Acetyltransferase in Complex with CoA
Authors
Lin, Y., Wagner, G.
Assembly
p55 monomer in complex with CoA
Entity
1. Tetrahymena histone acetyltransferase (polymer, Thiol state: all free), 166 monomers, 19698.71 Da Detail

MKGLLDFDIL TNDGTHRNMK LLIDLKNIFS RQLPKMPKEY IVKLVFDRHH ESMVILKNKQ KVIGGICFRQ YKPQRFAEVA FLAVTANEQV RGYGTRLMNK FKDHMQKQNI EYLLTYADNF AIGYFKKQGF TKEHRMPQEK WKGYIKDYDG GTLMECYIHP YVDYGN


2. COENZYME A (non-polymer), 767.534 Da
Total weight
20466.244 Da
Max. entity weight
19698.71 Da
Source organism
Tetrahymena thermophila
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 98.8 %, Completeness: 88.5 %, Completeness (bb): 96.3 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All88.5 % (1861 of 2103)92.1 % (1017 of 1104)82.7 % (671 of 811)92.0 % (173 of 188)
Backbone96.3 % (938 of 974)96.9 % (317 of 327)96.1 % (467 of 486)95.7 % (154 of 161)
Sidechain83.5 % (1071 of 1283)90.1 % (700 of 777)73.5 % (352 of 479)70.4 % (19 of 27)
Aromatic41.5 % (93 of 224)75.9 % (85 of 112) 6.3 % (7 of 111)100.0 % (1 of 1)
Methyl100.0 % (158 of 158)100.0 % (79 of 79)100.0 % (79 of 79)

1. Tetrahymena histone acetyltransferase

MKGLLDFDIL TNDGTHRNMK LLIDLKNIFS RQLPKMPKEY IVKLVFDRHH ESMVILKNKQ KVIGGICFRQ YKPQRFAEVA FLAVTANEQV RGYGTRLMNK FKDHMQKQNI EYLLTYADNF AIGYFKKQGF TKEHRMPQEK WKGYIKDYDG GTLMECYIHP YVDYGN

Sample

Temperature 300 (±1) K, pH 6.5 (±0.1)


#NameIsotope labelingTypeConcentration
1Tetrahymena histone acetyltransferase0.4 mM
2coenzyme A0.8 mM
3sodium phosphate50 mM
4NaCl100 mM
5DTT0.1 mM
6NaN30.1 mM
7EDTA0.1 mM

LACS Plot; CA
Referencing offset: -0.1 ppm, Outliers: 4 Detail
LACS Plot; CB
Referencing offset: -0.1 ppm, Outliers: 4 Detail
LACS Plot; HA
Referencing offset: -0.04 ppm, Outliers: 2 Detail
LACS Plot; CO
Referencing offset: 1.21 ppm, Outliers: 1 Detail
Release date
2000-12-13
Citation
Efficient side-chain and backbone assignment in large proteins: application to tGCN5
Lin, Y., Wagner, G.
J. Biomol. NMR (1999), 15, 227-239, PubMed 10677826 , DOI: ,
Related entities 1. Tetrahymena histone acetyltransferase, : 1 : 8 : 23 entities Detail
Experiments performed 1 experiments Detail