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Complete 1H, 13C, and 15N Chemical Shift Assignments for the N-terminal Domain of DNA Polymerase B (residues 2-87).
Authors
Liu, D., DeRose, E.F., Prasad, R., Wilson, S.H., Mullen, G.P.
Assembly
N-Terminal Domain of DNA Polymerase B
Entity
1. N-Terminal Domain of DNA Polymerase B (polymer), 87 monomers, 9612.135 Da Detail

MSKRKAPQET LNGGITDMLV ELANFEKNVS QAIHKYNAYR KAASVIAKYP HKIKSGAEAK KLPGVGTKIA EKIDEFLATG KLRKLEK


Formula weight
9612.135 Da
Source organism
Rattus norvegicus
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 98.9 %, Completeness: 85.9 %, Completeness (bb): 91.6 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All85.9 % (898 of 1045)90.7 % (499 of 550)78.6 % (316 of 402)89.2 % (83 of 93)
Backbone91.6 % (467 of 510)94.7 % (162 of 171)89.4 % (228 of 255)91.7 % (77 of 84)
Sidechain81.2 % (500 of 616)88.9 % (337 of 379)68.9 % (157 of 228)66.7 % (6 of 9)
Aromatic48.1 % (25 of 52)96.2 % (25 of 26) 0.0 % (0 of 26)
Methyl99.0 % (97 of 98)100.0 % (49 of 49)98.0 % (48 of 49)

1. DNA Polymerase B

MSKRKAPQET LNGGITDMLV ELANFEKNVS QAIHKYNAYR KAASVIAKYP HKIKSGAEAK KLPGVGTKIA EKIDEFLATG KLRKLEK

Sample #1

Temperature 298 (±0.5) K, pH 6.7 (±0.15), Details After purification the sample was eluted from a Sephadex G15 column (18 x 1.0 cm) with 5 mM Tris-d11, pH 7.5, 400 mM NaCl. Prior to performing NMR expeiments the pH was adjusted to 6.7.


#NameIsotope labelingTypeConcentration
1DNA Polymerase B[U-100% 13C; U-100% 15N]2.8 mM
2Tris-d115 mM
3NaCl400 mM
Sample #2

Temperature 298 (±0.5) K, pH 6.7 (±0.15)


#NameIsotope labelingTypeConcentration
4DNA Polymerase B[U-100% 15N]4.7 mM
5Tris-d115 mM
6NaCl400 mM
Sample #3

Temperature 298 (±0.5) K, pH 6.7 (±0.15)


#NameIsotope labelingTypeConcentration
7DNA Polymerase B1.4 mM
8Tris-d115 mM
9NaCl400 mM

LACS Plot; CA
Referencing offset: 0.32 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: 0.32 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: 0.07 ppm, Outliers: 2 Detail
LACS Plot; CO
Referencing offset: -0.37 ppm, Outliers: 1 Detail
Release date
1999-05-18
Citation
Assignments of 1H, 15N, and 13C Resonances for the Backbone and Side Chains of the N-Terminal Domain of DNA Polymerase B. Determination of the Secondary Structure and Tertiary Contacts
Liu, D., DeRose, E.F., Prasad, R., Wilson, S.H., Mullen, G.P.
Biochemistry (1994), 33, 9537-9545
Related entities 1. N-Terminal Domain of DNA Polymerase B, : 1 : 14 : 88 : 14 entities Detail
Interaction partners 1. N-Terminal Domain of DNA Polymerase B, : 2 interactors Detail