BMRBj - BMREntryMaster

Found 1 Document (0.747 seconds)

BMRB: 434

5MVZ

Complete Resonance Assignment for the Polypeptide Backbone of Interleukin 1beta Using Three-Dimensional Heteronuclear NMR Spectroscopy

Authors

Driscoll, P.C., Clore, G.MARIUS., Marion, D., Wingfield, P., Gronenborn, A.M.

Assembly

interleukin 1-beta

Entity

1. interleukin 1-beta (polymer), 153 monomers, 17376.66 Da Detail

APVRSLNCTL RDSQQKSLVM SGPYELKALH LQGQDMEQQV VFSMSFVQGE ESNDKIPVAL GLKEKNLYLS CVLKDDKPTL QLESVDPKNY PKKKMEKRFV FNKIEINNKL EFESAQFPNW YISTSQAENM PVFLGGTKGG QDITDFTMQF VSS

Formula weight

17376.66 Da

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.7 %, Completeness: 73.6 %, Completeness (bb): 98.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	73.6 % (840 of 1141)	71.6 % (697 of 974)	85.6 % (143 of 167)
Backbone	98.7 % (445 of 451)	98.7 % (302 of 306)	98.6 % (143 of 145)
Sidechain	57.2 % (395 of 690)	59.1 % (395 of 668)	0.0 % (0 of 22)
Aromatic	91.4 % (64 of 70)	92.8 % (64 of 69)	0.0 % (0 of 1)
Methyl	69.9 % (51 of 73)	69.9 % (51 of 73)

1. interleukin 1-beta

APVRSLNCTL RDSQQKSLVM SGPYELKALH LQGQDMEQQV VFSMSFVQGE ESNDKIPVAL GLKEKNLYLS CVLKDDKPTL QLESVDPKNY PKKKMEKRFV FNKIEINNKL EFESAQFPNW YISTSQAENM PVFLGGTKGG QDITDFTMQF VSS

Show sample condition

Sample

Temperature 309 K, pH 5.4

Release date

1995-07-30

Citation

Complete Resonance Assignment for the Polypeptide Backbone of Interleukin 1beta Using Three-Dimensional Heteronuclear NMR Spectroscopy
Driscoll, P.C., Clore, G.MARIUS., Marion, D., Wingfield, P., Gronenborn, A.M.
Biochemistry (1990), 29, 3542-3556, PubMed , DOI:

Related entities 1. interleukin 1-beta, : 1 : 47 : 7 : 5 : 83 entities Detail

Interaction partners 1. interleukin 1-beta, : 9 interactors Detail

More details for 9 interactors identified by 8 citations

Experiments performed 1 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr434_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr434_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	chemical_shift_assignment_data_set_one	Warning	926		99.3 (chain: 1, length: 153)

Assigned chemical shifts

Saveframe: chemical_shift_assignment_data_set_one
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 153, Sequence coverage: 99.3%
- Total number of assignments
  - ¹H chemical shifts: 782
  - ¹⁵N chemical shifts: 144
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	974	684	70.2
¹⁵N chemical shifts	170	144	84.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	668	380	56.9
¹⁵N chemical shifts	25	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	69	63	91.3
¹⁵N chemical shifts	1	0	0.0

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Found 1 Document (0.747 seconds)

BMRB: 434

Sample

Related entities 1. interleukin 1-beta, : 1 : 47 : 7 : 5 : 83 entities Detail

Interaction partners 1. interleukin 1-beta, : 9 interactors Detail

Experiments performed 1 experiments Detail

Instrument

Sample

Chemical shift validation 3 contents Detail

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	306	304	99.3
¹⁵N chemical shifts	145	144	99.3