BMRB: 4342

Assignment of the 1H, 15N, and 13C Resonances of the C-terminal Domain of Frataxin, the Protein Involved in Friedreich Ataxia.

Authors

Musco, G., de Tommasi, T., Stier, G., Kolmerer, B., Bottomle, M., Adinolfi, S., Musket, F., Gibson, T., Frenkiel, T., Pastore, A.

Assembly

Frataxin

Entity

1. Frataxin (polymer), 122 monomers, 13588.93 Da Detail

PMDETTYERL AEETLDSLAE FFEDLADKPY TFEDYDVSFG SGVLTVKLGG DLGTYVINKQ TPNKQIWLSS PSSGPKRYDW TGKNWVYSHD GVSLHELLAA ELTKALKTKL DLSSLAYSGK DA

Formula weight

13588.93 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 94.3 %, Completeness (bb): 93.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.3 % (1323 of 1403)	98.8 % (712 of 721)	87.4 % (487 of 557)	99.2 % (124 of 125)
Backbone	93.9 % (678 of 722)	99.2 % (246 of 248)	88.5 % (316 of 357)	99.1 % (116 of 117)
Sidechain	95.3 % (757 of 794)	98.5 % (466 of 473)	90.4 % (283 of 313)	100.0 % (8 of 8)
Aromatic	81.4 % (114 of 140)	100.0 % (70 of 70)	61.2 % (41 of 67)	100.0 % (3 of 3)
Methyl	99.2 % (131 of 132)	98.5 % (65 of 66)	100.0 % (66 of 66)

1. Frataxin

PMDETTYERL AEETLDSLAE FFEDLADKPY TFEDYDVSFG SGVLTVKLGG DLGTYVINKQ TPNKQIWLSS PSSGPKRYDW TGKNWVYSHD GVSLHELLAA ELTKALKTKL DLSSLAYSGK DA

Show sample condition

Sample

Temperature 300 (±1) K, pH 6.8 (±0.3)

#	Name	Isotope labeling	Type	Concentration
1	Frataxin	[U-95% 13C; U-90% 15N]		1.0 mM

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LACS Plot; CA

Referencing offset: -0.03 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	direct	0.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.03 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	direct	0.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.04 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	direct	0.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.4 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	direct	0.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 15 models in PDB: 1LY7, Strand ID: A Detail

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Release date

2000-01-04

Citation

Assignment of the 1H, 15N, and 13C resonances of the C-terminal domain of frataxin, the protein responsible for Friedreich ataxia
Musco, G., de Tommasi, T., Stier, G., Kolmerer, B., Bottomle, M., Adinolfi, S., Musket, F., Gibson, T., Frenkiel, T., Pastore, A.
J. Biomol. NMR (1999), 15, 87-88, PubMed 10549137 , DOI: , Abstract

Related entities 1. Frataxin, : 1 : 128 entities Detail

Interaction partners 1. Frataxin, : 4 interactors Detail

Experiments performed 9 experiments Detail