BMRBj - BMREntryMaster

Found 1 Document (0.57 seconds)

BMRB: 435

5MVZ

Complete Resonance Assignment for the Polypeptide Backbone of Interleukin 1beta Using Three-Dimensional Heteronuclear NMR Spectroscopy

Authors

Driscoll, P.C., Clore, G.MARIUS., Marion, D., Wingfield, P., Gronenborn, A.M.

Assembly

interleukin 1-beta

Entity

1. interleukin 1-beta (polymer), 152 monomers, 17305.58 Da Detail

PVRSLNCTLR DSQQKSLVMS GPYELKALHL QGQDMEQQVV FSMSFVQGEE SNDKIPVALG LKEKNLYLSC VLKDDKPTLQ LESVDPKNYP KKKMEKRFVF NKIEINNKLE FESAQFPNWY ISTSQAENMP VFLGGTKGGQ DITDFTMQFV SS

Formula weight

17305.58 Da

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.3 %, Completeness: 71.2 %, Completeness (bb): 98.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	71.2 % (809 of 1137)	68.5 % (665 of 971)	86.7 % (144 of 166)
Backbone	98.9 % (443 of 448)	98.4 % (299 of 304)	100.0 % (144 of 144)
Sidechain	53.1 % (366 of 689)	54.9 % (366 of 667)	0.0 % (0 of 22)
Aromatic	84.3 % (59 of 70)	85.5 % (59 of 69)	0.0 % (0 of 1)
Methyl	63.9 % (46 of 72)	63.9 % (46 of 72)

1. interleukin 1-beta

PVRSLNCTLR DSQQKSLVMS GPYELKALHL QGQDMEQQVV FSMSFVQGEE SNDKIPVALG LKEKNLYLSC VLKDDKPTLQ LESVDPKNYP KKKMEKRFVF NKIEINNKLE FESAQFPNWY ISTSQAENMP VFLGGTKGGQ DITDFTMQFV SS

Show sample condition

Sample

Temperature 309 K, pH 5.4

Release date

1995-07-30

Citation

Complete Resonance Assignment for the Polypeptide Backbone of Interleukin 1beta Using Three-Dimensional Heteronuclear NMR Spectroscopy
Driscoll, P.C., Clore, G.MARIUS., Marion, D., Wingfield, P., Gronenborn, A.M.
Biochemistry (1990), 29, 3542-3556, DOI 10.1021/bi00466a018

Related entities 1. interleukin 1-beta, : 1 : 47 : 7 : 5 : 81 entities Detail

Interaction partners 1. interleukin 1-beta, : 9 interactors Detail

More details for 9 interactors identified by 8 citations

Experiments performed 1 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr435_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr435_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	chemical_shift_assignment_data_set_one	Warning	901		99.3 (chain: 1, length: 152)

Assigned chemical shifts

Saveframe: chemical_shift_assignment_data_set_one
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 152, Sequence coverage: 99.3%
- Total number of assignments
  - ¹H chemical shifts: 757
  - ¹⁵N chemical shifts: 144
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

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Found 1 Document (0.57 seconds)

BMRB: 435

Sample

Related entities 1. interleukin 1-beta, : 1 : 47 : 7 : 5 : 81 entities Detail

Interaction partners 1. interleukin 1-beta, : 9 interactors Detail

Experiments performed 1 experiments Detail

Instrument

Sample

Chemical shift validation 3 contents Detail

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	971	665	68.5
¹⁵N chemical shifts	169	144	85.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	304	299	98.4
¹⁵N chemical shifts	144	144	100.0