BMRBj - BMREntryMaster

Found 1 Document (1.45 seconds)

BMRB: 4350

8BQF

1H,15N Resonance Assignment of Escherichia coli Adenylate Kinase with Its Bi-Substrate Analog AP5A

Authors

Burlacu-Miron, S., Gilles, A., Popescu, A., Barzu, O., Craescu, C.T.

Assembly

Adenylate kinase from E. coli

Entity

1. Adenylate kinase from E. coli (polymer), 214 monomers, 23585.72 Da Detail

MRIILLGAPG AGKGTQAQFI MEKYGIPQIS TGDMLRAAVK SGSELGKQAK DIMDAGKLVT DELVIALVKE RIAQEDCRNG FLLDGFPRTI PQADAMKEAG INVDYVLEFD VPDELIVDRI VGRRVHAPSG RVYHVKFNPP KVEGKDDVTG EELTTRKDDQ EETVRKRLVE YHQMTAPLIG YYSKEAEAGN TKYAKVDGTK PVAEVRADLE KILG

Formula weight

23585.72 Da

Source organism

Escherichia coli

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 89.7 %, Completeness: 53.7 %, Completeness (bb): 87.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	53.7 % (805 of 1498)	47.9 % (614 of 1282)	88.4 % (191 of 216)
Backbone	87.2 % (560 of 642)	84.2 % (369 of 438)	93.6 % (191 of 204)
Sidechain	28.7 % (246 of 856)	29.1 % (246 of 844)	0.0 % (0 of 12)
Aromatic	40.7 % (24 of 59)	40.7 % (24 of 59)
Methyl	42.2 % (54 of 128)	42.2 % (54 of 128)

1. Adenylate kinase from E. coli

Show sample condition

Sample #1

Temperature 308 K, pH 6.7

#	Name	Isotope labeling	Type	Concentration
1	Adenylate kinase from E. coli			1.4 mM

Sample #2

Temperature 308 K, pH 6.7, Details H2O:93%/D2O:7%

#	Name	Isotope labeling	Type	Concentration
2	Adenylate kinase from E. coli	[U-15N]		1.2 mM

Release date

1999-09-26

Citation

NMR Studies on Escherichia coli Adenylate Kinase, Free and in Complex with AP5A
Burlacu-Miron, S., Gilles, A., Popescu, A., Barzu, O., Craescu, C.T.
Eur. J. Biochem. (1999), 264, 765-774, PubMed , DOI:

Related entities 1. Adenylate kinase from E. coli, : 1 : 11 : 3 : 14 : 269 entities Detail

Interaction partners 1. Adenylate kinase from E. coli, : 11 interactors Detail

More details for 11 interactors identified by 2 citations

Experiments performed 6 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr4350_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	OK

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr4350_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	shift_AK	OK	832		88.8 (chain: 1, length: 214)
1	Chemical shift references	chemical_shift_reference	OK	2		No information

Assigned chemical shifts

Saveframe: shift_AK
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 214, Sequence coverage: 88.8%
- Total number of assignments
  - ¹H chemical shifts: 643
  - ¹⁵N chemical shifts: 189
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	1282	551	43.0
¹⁵N chemical shifts	229	189	82.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	438	363	82.9
¹⁵N chemical shifts	204	189	92.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	844	188	22.3
¹⁵N chemical shifts	25	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	134	46	34.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	59	21	35.6

Keywords Adenylate Kinase, NMPkinase, AKe, AP5A, NMR, nuclear magnetic resonance, protein, resonance assignment

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (1.45 seconds)

BMRB: 4350

Sample #1

Sample #2

Related entities 1. Adenylate kinase from E. coli, : 1 : 11 : 3 : 14 : 269 entities Detail

Interaction partners 1. Adenylate kinase from E. coli, : 11 interactors Detail

Experiments performed 6 experiments Detail

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Chemical shift validation 3 contents Detail