BMRBj - BMREntryMaster

Found 1 Document (1.429 seconds)

BMRB: 4358

1B72

NMR studies of the Pbx1 TALE Homeodomain Protein free in Solution and bound to DNA : Proposal for a Mechanism of HoxB1-Pbx1-DNA Complex Assembly

Authors

Jabet, C., Gitti, R., Summers, M.F., Wolberger, C.

Assembly

Pbx1 homeodomain

Entity

1. Pbx1 homeodomain (polymer), 87 monomers, 10055.19 Da Detail

ARRKRRNFNK QATEILNEYF YSHLSNPYPS EEAKEELAKK CGITVSQVSN WFGNKRIRYK KNIGKFQEEA NIYAAKTAVT ATNVSAH

Formula weight

10055.19 Da

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 85.1 %, Completeness: 21.9 %, Completeness (bb): 42.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	21.9 % (231 of 1054)	14.8 % (82 of 555)	19.2 % (77 of 401)	73.5 % (72 of 98)
Backbone	42.5 % (220 of 518)	42.3 % (74 of 175)	29.1 % (75 of 258)	83.5 % (71 of 85)
Sidechain	1.9 % (12 of 620)	2.1 % (8 of 380)	1.3 % (3 of 227)	7.7 % (1 of 13)
Aromatic	2.0 % (2 of 100)	2.0 % (1 of 50)	0.0 % (0 of 49)	100.0 % (1 of 1)
Methyl	5.1 % (4 of 78)	5.1 % (2 of 39)	5.1 % (2 of 39)

1. Pbx1

ARRKRRNFNK QATEILNEYF YSHLSNPYPS EEAKEELAKK CGITVSQVSN WFGNKRIRYK KNIGKFQEEA NIYAAKTAVT ATNVSAH

Show sample condition

Sample #1

Temperature 298 (±1) K, pH 7 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Pbx1	[U-90% 15N]		1 mM

Sample #2

Temperature 298 (±1) K, pH 7 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	Pbx1	[U-95% 13C; U-90% 15N]		1 mM

Release date

1999-11-29

Citation

NMR studies of the pbx1 TALE homeodomain protein free in solution and bound to DNA: proposal for a mechanism of HoxB1-Pbx1-DNA complex assembly
Jabet, C., Gitti, R., Summers, M.F., Wolberger, C.
J. Mol. Biol. (1999), 291, 521-530, PubMed 10448033 , DOI 10.1006/jmbi.1999.2983 , Abstract

Related entities 1. Pbx1 homeodomain, : 1 : 4 : 4 : 62 entities Detail

Interaction partners 1. Pbx1 homeodomain, : 14 interactors Detail

More details for 14 interactors identified by 8 citations

Experiments performed 4 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr4358_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	OK

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr4358_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assign_chemical_shifts_1	OK	215		85.1 (chain: 1, length: 87)
1	Chemical shift references	chemical_shift_reference	OK	3		No information

Assigned chemical shifts

Saveframe: assign_chemical_shifts_1
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 87, Sequence coverage: 85.1%
- Total number of assignments
  - ¹⁵N chemical shifts: 72
  - ¹H chemical shifts: 72
  - ¹³C chemical shifts: 71
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹⁵N chemical shifts	104	72	69.2
¹H chemical shifts	555	72	13.0
¹³C chemical shifts	401	71	17.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹⁵N chemical shifts	85	71	83.5
¹H chemical shifts	175	71	40.6
¹³C chemical shifts	174	71	40.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹⁵N chemical shifts	19	1	5.3
¹H chemical shifts	380	1	0.3
¹³C chemical shifts	227	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	39	0	0.0
¹³C chemical shifts	39	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹⁵N chemical shifts	1	1	100.0
¹H chemical shifts	50	1	2.0
¹³C chemical shifts	49	0	0.0

Keywords Hox, Pbx, homeodomain

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (1.429 seconds)

BMRB: 4358

Sample #1

Sample #2

Related entities 1. Pbx1 homeodomain, : 1 : 4 : 4 : 62 entities Detail

Interaction partners 1. Pbx1 homeodomain, : 14 interactors Detail

Experiments performed 4 experiments Detail

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Chemical shift validation 3 contents Detail