BMRBj - BMREntryMaster

Found 1 Document (0.956 seconds)

BMRB: 4359

1B72

NMR studies of the Pbx1 TALE Homeodomain Protein free in Solution and bound to DNA : Proposal for a Mechanism of HoxB1-Pbx1-DNA Complex Assembly

Authors

Jabet, C., Gitti, R., Summers, M.F., Wolberger, C.

Assembly

Pbx1 homeodomain

Entity

1. Pbx1 (polymer), 19 monomers, 5889.740 Da Detail

CTCTATGATT GATCGGCTG

2. Pbx1 (polymer), 19 monomers, 5885.772 Da Detail

CAGCCGATCA ATCATAGAG

3. Pbx1 (polymer, Thiol state: all free), 87 monomers, 10055.19 Da Detail

ARRKRRNFNK QATEILNEYF YSHLSNPYPS EEAKEELAKK CGITVSQVSN WFGNKRIRYK KNIGKFQEEA NIYAAKTAVT ATNVSAH

Total weight

21830.703 Da

Max. entity weight

10055.19 Da

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 60.8 %, Completeness: 16.4 %, Completeness (bb): 29.7 % Detail

Polymer type: polydeoxyribonucleotide polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	16.4 % (234 of 1424)	8.2 % (76 of 925)	20.7 % (83 of 401)	76.5 % (75 of 98)
Backbone	29.7 % (233 of 784)	17.2 % (76 of 441)	31.8 % (82 of 258)	88.2 % (75 of 85)
Sidechain	1.0 % (7 of 724)	0.0 % (0 of 484)	3.1 % (7 of 227)	0.0 % (0 of 13)
Aromatic	0.6 % (1 of 176)	0.0 % (0 of 126)	2.0 % (1 of 49)	0.0 % (0 of 1)
Methyl	0.0 % (0 of 88)	0.0 % (0 of 49)	0.0 % (0 of 39)

1. Pbx1

CTCTATGATT GATCGGCTG

2. Pbx1

CAGCCGATCA ATCATAGAG

3. Pbx1

ARRKRRNFNK QATEILNEYF YSHLSNPYPS EEAKEELAKK CGITVSQVSN WFGNKRIRYK KNIGKFQEEA NIYAAKTAVT ATNVSAH

Show sample condition

Sample #1

Temperature 298 (±1) K, pH 7 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Pbx1 homeodomain	[U-90% 15N]		1 mM

Sample #2

Temperature 298 (±1) K, pH 7 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	Pbx1 homeodomain	[U-95% 13C; U-90% 15N]		1 mM

Release date

1999-11-29

Citation

NMR studies of the pbx1 TALE homeodomain protein free in solution and bound to DNA: proposal for a mechanism of HoxB1-Pbx1-DNA complex assembly
Jabet, C., Gitti, R., Summers, M.F., Wolberger, C.
J. Mol. Biol. (1999), 291, 521-530, PubMed 10448033 , DOI 10.1006/jmbi.1999.2983 , Abstract

Related entities 3. Pbx1, : 1 : 4 : 4 : 62 entities Detail

Interaction partners 3. Pbx1, : 14 interactors Detail

More details for 14 interactors identified by 8 citations

Experiments performed 4 experiments Detail

Chemical shift validation 5 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr4359_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr4359_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assign_chemical_shifts_1	Warning	219		86.2 (chain: 3, length: 87)
1	Chemical shift references	chemical_shift_reference	OK	3		No information

Assigned chemical shifts

Saveframe: assign_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 3, Chain length: 87, Sequence coverage: 86.2%
- Total number of assignments
  - ¹⁵N chemical shifts: 74
  - ¹H chemical shifts: 74
  - ¹³C chemical shifts: 71
- Completeness of assignments
  - Entity_assemble_ID: 3
- Redox state of CYS
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹⁵N chemical shifts	104	74	71.2
¹H chemical shifts	555	74	13.3
¹³C chemical shifts	401	71	17.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹⁵N chemical shifts	85	74	87.1
¹H chemical shifts	175	74	42.3
¹³C chemical shifts	174	71	40.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹⁵N chemical shifts	19	0	0.0
¹H chemical shifts	380	0	0.0
¹³C chemical shifts	227	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	39	0	0.0
¹³C chemical shifts	39	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹⁵N chemical shifts	1	0	0.0
¹H chemical shifts	50	0	0.0
¹³C chemical shifts	49	0	0.0

Keywords Hox, Pbx, homeodomain

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Found 1 Document (0.956 seconds)

BMRB: 4359

Sample #1

Sample #2

Related entities 3. Pbx1, : 1 : 4 : 4 : 62 entities Detail

Interaction partners 3. Pbx1, : 14 interactors Detail

Experiments performed 4 experiments Detail

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Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

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Instrument

Sample #1

Sample #2

Chemical shift validation 5 contents Detail