NMR Solution Structure of the Human Prion Protein Domain reveals Species Dependent Structural Disorder and Intramolecular Association with the Flexible Tail
GSVVGGLGGY MLGSAMSRPI IHFGSDYEDR YYRENMHRYP NQVYYRPMDE YSNQNNFVHD CVNITIKQHT VTTTTKGENF TETDVKMMER VVEQMCITQY ERESQAYYQR GS
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 87.2 % (1141 of 1308) | 97.2 % (663 of 682) | 71.8 % (361 of 503) | 95.1 % (117 of 123) |
Backbone | 81.2 % (541 of 666) | 97.0 % (223 of 230) | 66.1 % (216 of 327) | 93.6 % (102 of 109) |
Sidechain | 91.9 % (685 of 745) | 95.6 % (432 of 452) | 85.7 % (239 of 279) | 100.0 % (14 of 14) |
Aromatic | 73.1 % (98 of 134) | 94.0 % (63 of 67) | 52.2 % (35 of 67) | |
Methyl | 98.8 % (85 of 86) | 97.7 % (42 of 43) | 100.0 % (43 of 43) |
1. human prion protein
GSVVGGLGGY MLGSAMSRPI IHFGSDYEDR YYRENMHRYP NQVYYRPMDE YSNQNNFVHD CVNITIKQHT VTTTTKGENF TETDVKMMER VVEQMCITQY ERESQAYYQR GSTemperature 293 (±1) K, pH 4.5 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | human prion protein | 1.0 mM |
Temperature 293 (±1) K, pH 4.5 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
2 | human prion protein | [U-15N] | 1.0 mM |
Temperature 293 (±1) K, pH 4.5 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
3 | human prion protein | [U-15N; U-13C] | 1.0 mM |