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1Z1D

1H, 15N and 13C Resonance Assignments for the C-terminal Protein Interaction Region of the 32 kDa Subunit of Human Replication Protein A

Authors

Mer, G., Edwards, A.M., Chazin, W.J.

Assembly

Replication Protein A 32kDa subunit

Entity

1. Replication Protein A 32kDa subunit (polymer, Thiol state: all free), 103 monomers, 10988.04 Da Detail

GSHMANSQPS AGRAPISNPG MSEAGNFGGN SFMPANGLTV AQNQVLNLIK ACPRPEGLNF QDLKNQLKHM SVSSIKQAVD FLSNEGHIYS TVDDDHFKST DAE

Formula weight

10988.04 Da

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.1 %, Completeness: 83.1 %, Completeness (bb): 82.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	83.1 % (944 of 1136)	91.4 % (540 of 591)	68.8 % (297 of 432)	94.7 % (107 of 113)
Backbone	82.7 % (501 of 606)	95.2 % (199 of 209)	69.7 % (209 of 300)	95.9 % (93 of 97)
Sidechain	85.6 % (534 of 624)	89.3 % (341 of 382)	79.2 % (179 of 226)	87.5 % (14 of 16)
Aromatic	36.5 % (27 of 74)	73.0 % (27 of 37)	0.0 % (0 of 37)
Methyl	98.9 % (87 of 88)	97.7 % (43 of 44)	100.0 % (44 of 44)

1. Replication Protein A

GSHMANSQPS AGRAPISNPG MSEAGNFGGN SFMPANGLTV AQNQVLNLIK ACPRPEGLNF QDLKNQLKHM SVSSIKQAVD FLSNEGHIYS TVDDDHFKST DAE

Show sample condition

Sample

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	Replication Protein A	[U-13C; U-15N]	1 mM
2	phosphate buffer		25 mM
3	DTT		5 mM
4	H2O		93 %
5	D2O		7 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	0.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.06 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	0.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.06 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	0.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.04 ppm, Outliers: 1 Detail

Release date

2005-04-24

Citation 1

1H, 15N and 13C resonance assignments for the C-terminal protein interaction region of the 32 kDa subunit of human replication protein A
Mer, G., Edwards, A.M., Chazin, W.J.
J. Biomol. NMR (2000), 17, 179-180, PubMed 10921783 , DOI: , Abstract

Show more 1 citaion

Citation 2

The primary structure of the 32-kDa subunit of human replication protein A
Erdile, L.F., Wold, M.S., Kelly, T.J.
J. Biol. Chem. (1990), 265, 3177-3182, PubMed 2406247 , DOI: , Abstract

Hide non-primary 1 citaion

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: P15927 released on 1990-04-01
Title RFA2_HUMAN Entity Replication protein A 32 kDa subunit

Related entities 1. Replication Protein A 32kDa subunit, : 1 : 5 : 12 entities Detail

Interaction partners 1. Replication Protein A 32kDa subunit, : 94 interactors Detail

More details for 94 interactors identified by 29 citations

Experiments performed 17 experiments Detail

1 2D 1H-15N HSQC

Instrument

#1 Model Bruker AMX (500 MHz)

#2 Model Bruker AMX (600 MHz)

#3 Model Bruker DRX (600 MHz)

#4 Model Bruker DMX (750 MHz)

#5 Model Bruker DRX (800 MHz)

Sample #1

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	Replication Protein A	[U-13C; U-15N]	1 mM
2	phosphate buffer		25 mM
3	DTT		5 mM
4	H2O		93 %
5	D2O		7 %

Sample #2

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
6	Replication Protein A	[U-10% 13C; U-15N]	1 mM
7	phosphate buffer		25 mM
8	DTT		5 mM
9	H2O		93 %
10	D2O		7 %

Sample #3

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
11	Replication Protein A	[U-15N]	1 mM
12	phosphate buffer		25 mM
13	DTT		5 mM
14	H2O		93 %
15	D2O		7 %

Sample #4

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
16	Replication Protein A	NA	1 mM
17	phosphate buffer		25 mM
18	DTT		5 mM
19	H2O		93 %
20	D2O		7 %

2 2D 1H-13C HSQC

Instrument

#1 Model Bruker AMX (500 MHz)

#2 Model Bruker AMX (600 MHz)

#3 Model Bruker DRX (600 MHz)

#4 Model Bruker DMX (750 MHz)

#5 Model Bruker DRX (800 MHz)

Sample #1

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	Replication Protein A	[U-13C; U-15N]	1 mM
2	phosphate buffer		25 mM
3	DTT		5 mM
4	H2O		93 %
5	D2O		7 %

Sample #2

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
6	Replication Protein A	[U-10% 13C; U-15N]	1 mM
7	phosphate buffer		25 mM
8	DTT		5 mM
9	H2O		93 %
10	D2O		7 %

Sample #3

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
11	Replication Protein A	[U-15N]	1 mM
12	phosphate buffer		25 mM
13	DTT		5 mM
14	H2O		93 %
15	D2O		7 %

Sample #4

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
16	Replication Protein A	NA	1 mM
17	phosphate buffer		25 mM
18	DTT		5 mM
19	H2O		93 %
20	D2O		7 %

3 2D NOESY

Instrument

#1 Model Bruker AMX (500 MHz)

#2 Model Bruker AMX (600 MHz)

#3 Model Bruker DRX (600 MHz)

#4 Model Bruker DMX (750 MHz)

#5 Model Bruker DRX (800 MHz)

Sample #1

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	Replication Protein A	[U-13C; U-15N]	1 mM
2	phosphate buffer		25 mM
3	DTT		5 mM
4	H2O		93 %
5	D2O		7 %

Sample #2

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
6	Replication Protein A	[U-10% 13C; U-15N]	1 mM
7	phosphate buffer		25 mM
8	DTT		5 mM
9	H2O		93 %
10	D2O		7 %

Sample #3

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
11	Replication Protein A	[U-15N]	1 mM
12	phosphate buffer		25 mM
13	DTT		5 mM
14	H2O		93 %
15	D2O		7 %

Sample #4

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
16	Replication Protein A	NA	1 mM
17	phosphate buffer		25 mM
18	DTT		5 mM
19	H2O		93 %
20	D2O		7 %

4 2D DQF-COSY

Instrument

#1 Model Bruker AMX (500 MHz)

#2 Model Bruker AMX (600 MHz)

#3 Model Bruker DRX (600 MHz)

#4 Model Bruker DMX (750 MHz)

#5 Model Bruker DRX (800 MHz)

Sample #1

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	Replication Protein A	[U-13C; U-15N]	1 mM
2	phosphate buffer		25 mM
3	DTT		5 mM
4	H2O		93 %
5	D2O		7 %

Sample #2

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
6	Replication Protein A	[U-10% 13C; U-15N]	1 mM
7	phosphate buffer		25 mM
8	DTT		5 mM
9	H2O		93 %
10	D2O		7 %

Sample #3

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
11	Replication Protein A	[U-15N]	1 mM
12	phosphate buffer		25 mM
13	DTT		5 mM
14	H2O		93 %
15	D2O		7 %

Sample #4

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
16	Replication Protein A	NA	1 mM
17	phosphate buffer		25 mM
18	DTT		5 mM
19	H2O		93 %
20	D2O		7 %

5 2D TOCSY

Instrument

#1 Model Bruker AMX (500 MHz)

#2 Model Bruker AMX (600 MHz)

#3 Model Bruker DRX (600 MHz)

#4 Model Bruker DMX (750 MHz)

#5 Model Bruker DRX (800 MHz)

Sample #1

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	Replication Protein A	[U-13C; U-15N]	1 mM
2	phosphate buffer		25 mM
3	DTT		5 mM
4	H2O		93 %
5	D2O		7 %

Sample #2

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
6	Replication Protein A	[U-10% 13C; U-15N]	1 mM
7	phosphate buffer		25 mM
8	DTT		5 mM
9	H2O		93 %
10	D2O		7 %

Sample #3

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
11	Replication Protein A	[U-15N]	1 mM
12	phosphate buffer		25 mM
13	DTT		5 mM
14	H2O		93 %
15	D2O		7 %

Sample #4

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
16	Replication Protein A	NA	1 mM
17	phosphate buffer		25 mM
18	DTT		5 mM
19	H2O		93 %
20	D2O		7 %

6 2D 2Q

Instrument

#1 Model Bruker AMX (500 MHz)

#2 Model Bruker AMX (600 MHz)

#3 Model Bruker DRX (600 MHz)

#4 Model Bruker DMX (750 MHz)

#5 Model Bruker DRX (800 MHz)

Sample #1

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	Replication Protein A	[U-13C; U-15N]	1 mM
2	phosphate buffer		25 mM
3	DTT		5 mM
4	H2O		93 %
5	D2O		7 %

Sample #2

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
6	Replication Protein A	[U-10% 13C; U-15N]	1 mM
7	phosphate buffer		25 mM
8	DTT		5 mM
9	H2O		93 %
10	D2O		7 %

Sample #3

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
11	Replication Protein A	[U-15N]	1 mM
12	phosphate buffer		25 mM
13	DTT		5 mM
14	H2O		93 %
15	D2O		7 %

Sample #4

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
16	Replication Protein A	NA	1 mM
17	phosphate buffer		25 mM
18	DTT		5 mM
19	H2O		93 %
20	D2O		7 %

7 3D HNCACB

Instrument

#1 Model Bruker AMX (500 MHz)

#2 Model Bruker AMX (600 MHz)

#3 Model Bruker DRX (600 MHz)

#4 Model Bruker DMX (750 MHz)

#5 Model Bruker DRX (800 MHz)

Sample #1

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	Replication Protein A	[U-13C; U-15N]	1 mM
2	phosphate buffer		25 mM
3	DTT		5 mM
4	H2O		93 %
5	D2O		7 %

Sample #2

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
6	Replication Protein A	[U-10% 13C; U-15N]	1 mM
7	phosphate buffer		25 mM
8	DTT		5 mM
9	H2O		93 %
10	D2O		7 %

Sample #3

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
11	Replication Protein A	[U-15N]	1 mM
12	phosphate buffer		25 mM
13	DTT		5 mM
14	H2O		93 %
15	D2O		7 %

Sample #4

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
16	Replication Protein A	NA	1 mM
17	phosphate buffer		25 mM
18	DTT		5 mM
19	H2O		93 %
20	D2O		7 %

8 3D CBCA(CO)NH

Instrument

#1 Model Bruker AMX (500 MHz)

#2 Model Bruker AMX (600 MHz)

#3 Model Bruker DRX (600 MHz)

#4 Model Bruker DMX (750 MHz)

#5 Model Bruker DRX (800 MHz)

Sample #1

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	Replication Protein A	[U-13C; U-15N]	1 mM
2	phosphate buffer		25 mM
3	DTT		5 mM
4	H2O		93 %
5	D2O		7 %

Sample #2

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
6	Replication Protein A	[U-10% 13C; U-15N]	1 mM
7	phosphate buffer		25 mM
8	DTT		5 mM
9	H2O		93 %
10	D2O		7 %

Sample #3

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
11	Replication Protein A	[U-15N]	1 mM
12	phosphate buffer		25 mM
13	DTT		5 mM
14	H2O		93 %
15	D2O		7 %

Sample #4

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
16	Replication Protein A	NA	1 mM
17	phosphate buffer		25 mM
18	DTT		5 mM
19	H2O		93 %
20	D2O		7 %

9 3D C(CO)NH-TOCSY

Instrument

#1 Model Bruker AMX (500 MHz)

#2 Model Bruker AMX (600 MHz)

#3 Model Bruker DRX (600 MHz)

#4 Model Bruker DMX (750 MHz)

#5 Model Bruker DRX (800 MHz)

Sample #1

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	Replication Protein A	[U-13C; U-15N]	1 mM
2	phosphate buffer		25 mM
3	DTT		5 mM
4	H2O		93 %
5	D2O		7 %

Sample #2

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
6	Replication Protein A	[U-10% 13C; U-15N]	1 mM
7	phosphate buffer		25 mM
8	DTT		5 mM
9	H2O		93 %
10	D2O		7 %

Sample #3

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
11	Replication Protein A	[U-15N]	1 mM
12	phosphate buffer		25 mM
13	DTT		5 mM
14	H2O		93 %
15	D2O		7 %

Sample #4

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
16	Replication Protein A	NA	1 mM
17	phosphate buffer		25 mM
18	DTT		5 mM
19	H2O		93 %
20	D2O		7 %

10 3D H(CCO)NH-TOCSY

Instrument

#1 Model Bruker AMX (500 MHz)

#2 Model Bruker AMX (600 MHz)

#3 Model Bruker DRX (600 MHz)

#4 Model Bruker DMX (750 MHz)

#5 Model Bruker DRX (800 MHz)

Sample #1

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	Replication Protein A	[U-13C; U-15N]	1 mM
2	phosphate buffer		25 mM
3	DTT		5 mM
4	H2O		93 %
5	D2O		7 %

Sample #2

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
6	Replication Protein A	[U-10% 13C; U-15N]	1 mM
7	phosphate buffer		25 mM
8	DTT		5 mM
9	H2O		93 %
10	D2O		7 %

Sample #3

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
11	Replication Protein A	[U-15N]	1 mM
12	phosphate buffer		25 mM
13	DTT		5 mM
14	H2O		93 %
15	D2O		7 %

Sample #4

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
16	Replication Protein A	NA	1 mM
17	phosphate buffer		25 mM
18	DTT		5 mM
19	H2O		93 %
20	D2O		7 %

11 3D HBHA(CBCACO)NH

Instrument

#1 Model Bruker AMX (500 MHz)

#2 Model Bruker AMX (600 MHz)

#3 Model Bruker DRX (600 MHz)

#4 Model Bruker DMX (750 MHz)

#5 Model Bruker DRX (800 MHz)

Sample #1

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	Replication Protein A	[U-13C; U-15N]	1 mM
2	phosphate buffer		25 mM
3	DTT		5 mM
4	H2O		93 %
5	D2O		7 %

Sample #2

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
6	Replication Protein A	[U-10% 13C; U-15N]	1 mM
7	phosphate buffer		25 mM
8	DTT		5 mM
9	H2O		93 %
10	D2O		7 %

Sample #3

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
11	Replication Protein A	[U-15N]	1 mM
12	phosphate buffer		25 mM
13	DTT		5 mM
14	H2O		93 %
15	D2O		7 %

Sample #4

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
16	Replication Protein A	NA	1 mM
17	phosphate buffer		25 mM
18	DTT		5 mM
19	H2O		93 %
20	D2O		7 %

12 3D HNHA

Instrument

#1 Model Bruker AMX (500 MHz)

#2 Model Bruker AMX (600 MHz)

#3 Model Bruker DRX (600 MHz)

#4 Model Bruker DMX (750 MHz)

#5 Model Bruker DRX (800 MHz)

Sample #1

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	Replication Protein A	[U-13C; U-15N]	1 mM
2	phosphate buffer		25 mM
3	DTT		5 mM
4	H2O		93 %
5	D2O		7 %

Sample #2

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
6	Replication Protein A	[U-10% 13C; U-15N]	1 mM
7	phosphate buffer		25 mM
8	DTT		5 mM
9	H2O		93 %
10	D2O		7 %

Sample #3

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
11	Replication Protein A	[U-15N]	1 mM
12	phosphate buffer		25 mM
13	DTT		5 mM
14	H2O		93 %
15	D2O		7 %

Sample #4

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
16	Replication Protein A	NA	1 mM
17	phosphate buffer		25 mM
18	DTT		5 mM
19	H2O		93 %
20	D2O		7 %

13 3D HNHB

Instrument

#1 Model Bruker AMX (500 MHz)

#2 Model Bruker AMX (600 MHz)

#3 Model Bruker DRX (600 MHz)

#4 Model Bruker DMX (750 MHz)

#5 Model Bruker DRX (800 MHz)

Sample #1

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	Replication Protein A	[U-13C; U-15N]	1 mM
2	phosphate buffer		25 mM
3	DTT		5 mM
4	H2O		93 %
5	D2O		7 %

Sample #2

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
6	Replication Protein A	[U-10% 13C; U-15N]	1 mM
7	phosphate buffer		25 mM
8	DTT		5 mM
9	H2O		93 %
10	D2O		7 %

Sample #3

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
11	Replication Protein A	[U-15N]	1 mM
12	phosphate buffer		25 mM
13	DTT		5 mM
14	H2O		93 %
15	D2O		7 %

Sample #4

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
16	Replication Protein A	NA	1 mM
17	phosphate buffer		25 mM
18	DTT		5 mM
19	H2O		93 %
20	D2O		7 %

14 3D HACAHB-COSY

Instrument

#1 Model Bruker AMX (500 MHz)

#2 Model Bruker AMX (600 MHz)

#3 Model Bruker DRX (600 MHz)

#4 Model Bruker DMX (750 MHz)

#5 Model Bruker DRX (800 MHz)

Sample #1

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	Replication Protein A	[U-13C; U-15N]	1 mM
2	phosphate buffer		25 mM
3	DTT		5 mM
4	H2O		93 %
5	D2O		7 %

Sample #2

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
6	Replication Protein A	[U-10% 13C; U-15N]	1 mM
7	phosphate buffer		25 mM
8	DTT		5 mM
9	H2O		93 %
10	D2O		7 %

Sample #3

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
11	Replication Protein A	[U-15N]	1 mM
12	phosphate buffer		25 mM
13	DTT		5 mM
14	H2O		93 %
15	D2O		7 %

Sample #4

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
16	Replication Protein A	NA	1 mM
17	phosphate buffer		25 mM
18	DTT		5 mM
19	H2O		93 %
20	D2O		7 %

15 3D 15N edited TOCSY

Instrument

#1 Model Bruker AMX (500 MHz)

#2 Model Bruker AMX (600 MHz)

#3 Model Bruker DRX (600 MHz)

#4 Model Bruker DMX (750 MHz)

#5 Model Bruker DRX (800 MHz)

Sample #1

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	Replication Protein A	[U-13C; U-15N]	1 mM
2	phosphate buffer		25 mM
3	DTT		5 mM
4	H2O		93 %
5	D2O		7 %

Sample #2

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
6	Replication Protein A	[U-10% 13C; U-15N]	1 mM
7	phosphate buffer		25 mM
8	DTT		5 mM
9	H2O		93 %
10	D2O		7 %

Sample #3

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
11	Replication Protein A	[U-15N]	1 mM
12	phosphate buffer		25 mM
13	DTT		5 mM
14	H2O		93 %
15	D2O		7 %

Sample #4

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
16	Replication Protein A	NA	1 mM
17	phosphate buffer		25 mM
18	DTT		5 mM
19	H2O		93 %
20	D2O		7 %

16 3D 15N edited NOESY

Instrument

#1 Model Bruker AMX (500 MHz)

#2 Model Bruker AMX (600 MHz)

#3 Model Bruker DRX (600 MHz)

#4 Model Bruker DMX (750 MHz)

#5 Model Bruker DRX (800 MHz)

Sample #1

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	Replication Protein A	[U-13C; U-15N]	1 mM
2	phosphate buffer		25 mM
3	DTT		5 mM
4	H2O		93 %
5	D2O		7 %

Sample #2

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
6	Replication Protein A	[U-10% 13C; U-15N]	1 mM
7	phosphate buffer		25 mM
8	DTT		5 mM
9	H2O		93 %
10	D2O		7 %

Sample #3

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
11	Replication Protein A	[U-15N]	1 mM
12	phosphate buffer		25 mM
13	DTT		5 mM
14	H2O		93 %
15	D2O		7 %

Sample #4

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
16	Replication Protein A	NA	1 mM
17	phosphate buffer		25 mM
18	DTT		5 mM
19	H2O		93 %
20	D2O		7 %

17 3D 13C edited NOESY

Instrument

#1 Model Bruker AMX (500 MHz)

#2 Model Bruker AMX (600 MHz)

#3 Model Bruker DRX (600 MHz)

#4 Model Bruker DMX (750 MHz)

#5 Model Bruker DRX (800 MHz)

Sample #1

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	Replication Protein A	[U-13C; U-15N]	1 mM
2	phosphate buffer		25 mM
3	DTT		5 mM
4	H2O		93 %
5	D2O		7 %

Sample #2

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
6	Replication Protein A	[U-10% 13C; U-15N]	1 mM
7	phosphate buffer		25 mM
8	DTT		5 mM
9	H2O		93 %
10	D2O		7 %

Sample #3

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
11	Replication Protein A	[U-15N]	1 mM
12	phosphate buffer		25 mM
13	DTT		5 mM
14	H2O		93 %
15	D2O		7 %

Sample #4

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
16	Replication Protein A	NA	1 mM
17	phosphate buffer		25 mM
18	DTT		5 mM
19	H2O		93 %
20	D2O		7 %

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr4460_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr4460_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_label	Warning	1012		96.1 (chain: 1, length: 103)
1	Chemical shift references	chemical_shift_reference	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_label
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 103, Sequence coverage: 96.1%
- Total number of assignments
  - ¹H chemical shifts: 631
  - ¹³C chemical shifts: 277
  - ¹⁵N chemical shifts: 104
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Keywords DNA replication, DNA recombination, DNA repair

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	591	543	91.9
¹³C chemical shifts	432	277	64.1
¹⁵N chemical shifts	115	104	90.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	209	197	94.3
¹³C chemical shifts	206	99	48.1
¹⁵N chemical shifts	97	90	92.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	382	346	90.6
¹³C chemical shifts	226	178	78.8
¹⁵N chemical shifts	18	14	77.8

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Found 1 Document (3.193 seconds)

BMRB: 4460

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. Replication Protein A 32kDa subunit, : 1 : 5 : 12 entities Detail

Interaction partners 1. Replication Protein A 32kDa subunit, : 94 interactors Detail

Experiments performed 17 experiments Detail

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