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Found 1 Document (1.759 seconds)

BMRB: 4467

1UB1

Sequence-specific 1H, 13C, and 15N Assignments of the MAR-binding Domain of Chicken MeCP2/ARBP

Authors

Brunner, E., Weitzel, J., Heitmann, B., Maurer, T., Straetling, W.H., Kalbitzer, H.

Assembly

chicken MeCP2/ARBP monomer

Entity

1. chicken MeCP2/ARBP monomer (polymer, Thiol state: not present), 125 monomers, 13771.37 Da Detail

APAVPEASAS PKQRRSIIRD RGPMYDDPTL PEGWTRKLKQ RKSGRSAGKY DVYLINPQGK AFRSKVELIA YFEKVGDTSL DPNDFDFTVT GRGSPSRREQ RPPKKAKSPK SPGSGRGRGR PKGSG

Formula weight

13771.37 Da

Source organism

Gallus gallus

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.2 %, Completeness: 46.6 %, Completeness (bb): 91.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	46.6 % (676 of 1450)	31.2 % (243 of 780)	58.7 % (324 of 552)	92.4 % (109 of 118)
Backbone	91.8 % (663 of 722)	93.2 % (233 of 250)	88.9 % (321 of 361)	98.2 % (109 of 111)
Sidechain	12.5 % (105 of 839)	1.9 % (10 of 530)	31.5 % (95 of 302)	0.0 % (0 of 7)
Aromatic	0.0 % (0 of 84)	0.0 % (0 of 42)	0.0 % (0 of 41)	0.0 % (0 of 1)
Methyl	11.0 % (9 of 82)	4.9 % (2 of 41)	17.1 % (7 of 41)

1. Methyl-CpG-binding protein, attachment region binding protein

APAVPEASAS PKQRRSIIRD RGPMYDDPTL PEGWTRKLKQ RKSGRSAGKY DVYLINPQGK AFRSKVELIA YFEKVGDTSL DPNDFDFTVT GRGSPSRREQ RPPKKAKSPK SPGSGRGRGR PKGSG

Show sample condition

Sample #1

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Methyl-CpG-binding protein, attachment region binding protein	[U-15N; U-13C]		2.0 mM

Sample #2

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Methyl-CpG-binding protein, attachment region binding protein	[U-15N]		2.0 mM

Sample #3

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Methyl-CpG-binding protein, attachment region binding protein			2.0 mM

Sample #4

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	Methyl-CpG-binding protein, attachment region binding protein	[U-2H; U-13C; U-15N]		2.0 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.38 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.38 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.1 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.32 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 1UB1, Strand ID: A Detail

Release date

2000-12-14

Citation 1

Sequence-specific 1H, 13C, and 15N assignments of the MAR-binding domain of chicken MeCP2/ARBP
Brunner, E., Weitzel, J., Heitmann, B., Maurer, T., Straetling, W.H., Kalbitzer, H.
J. Biomol. NMR (2000), 17, 175-176, PubMed 10921781 , Abstract

Show more 10 citaions

Citation 2

Rett syndrome is caused by mutations in X-linked MECP2, encoding methyl-CpG-binding protein 2
Amir, R.E., Van den Veyver, I.B., Wan, M., Tran, C.Q., Francke, U., Zoghbi, H.Y.
Nat. Genet. (1999), 23, 185-188, PubMed 10508514 , DOI 10.1038/13810 , Abstract

Citation 3

Methylated DNA and MeCP2 recruit histone deacetylase to repress transcription
Jones, P.L., Veenstra, G.J., Wade, P.A., Vermaak, D., Kass, S.U., Landsberger, N., Strouboulis, J., Wolffe, A.P.
Nat. Genet. (1998), 19, 187-191, PubMed 9620779 , DOI 10.1038/561 , Abstract

Citation 4

Purification, sequence, and cellular localization of a novel chromosomal protein that binds to methylated DNA
Lewis, J.D., Meehan, R.R., Henzel, W.J., Maurer-Fogy, I., Jeppesen, P., Klein, F., Bird, A.
Cell (1992), 69, 905-914, PubMed 1606614 , Abstract

Citation 5

Dissection of the methyl-CpG binding domain from the chromosomal protein MeCP2
Nan, X., Meehan, R.R., Bird, A.
Nucleic Acids Res. (1993), 21, 4886-4892, PubMed 8177735 , Abstract

Citation 6

MeCP2 is a transcriptional repressor with abundant binding sites in genomic chromatin
Nan, X., Campoy, F.J., Bird, A.
Cell (1997), 88, 471-481, PubMed 9038338 , Abstract

Citation 7

Transcriptional repression by the methyl-CpG-binding protein MeCP2 involves a histone deacetylase complex
Nan, X., Ng, H.H., Johnson, C.A., Laherty, C.D., Turner, B.M., Eisenman, R.N., Bird, A.
Nature (1998), 393, 386-389, PubMed 9620804 , DOI 10.1038/30764 , Abstract

Citation 8

A matrix/scaffold attachment region binding protein: identification, purification, and mode of binding
von Kries, J.P., Buhrmester, H., Stratling, W.H.
Cell (1991), 64, 123-135, PubMed 1846084 , Abstract

Citation 9

The solution structure of the domain from MeCP2 that binds to methylated DNA
Wakefield, R.I., Smith, B.O., Nan, X., Free, A., Soteriou, A., Uhrin, D., Bird, A.P., Barlow, P.N.
J. Mol. Biol. (1999), 291, 1055-1065, PubMed 10518942 , DOI 10.1006/jmbi.1999.3023 , Abstract

Citation 10

Chicken MAR-binding protein ARBP is homologous to rat methyl-CpG-binding protein MeCP2
Weitzel, J.M., Buhrmester, H., Stratling, W.H.
Mol. Cell. Biol. (1997), 17, 5656-5666, PubMed 9271441 , Abstract

Citation 11

The 13C chemical-shift index: a simple method for the identification of protein secondary structure using 13C chemical-shift data
Wishart, D.S., Sykes, B.D.
J. Biomol. NMR (1994), 4, 171-180, PubMed 8019132 , Abstract

Hide non-primary 10 citaions

Entries sharing articles BMRB: 5, Swiss-Prot: 2 entries Detail

  BMRB: 4573 released on 2004-05-14
    Title 1HN,15N,13CO,13Ca,13Cb chemical shifts of 7,8-dihydroneopterin aldolase (DHNA) from Staphylococcus aureus
  BMRB: 4376 released on 1999-10-12
    Title Chemical shift assignments, 3JHNHA coupling constants, secondary structure and 15N{1H} Heteronuclear NOE values of the N-domain of VAT (VCP like ATPase of Thermoplasma). A group II AAA ATPase.
  BMRB: 4280 released on 1999-10-05
    Title Sequence Specific 1H, 13C and 15N Assignment of the Methyl Binding Domain of the Methyl-CpG-binding Protein MeCP2.
  BMRB: 4130 released on 1998-08-11
    Title 1H, 15N, 13C Chemical Shifts of Recombinant Rat Ferrocytochrome b5, A conformation
  BMRB: 4131 released on 1998-08-11
    Title 1H, 15N, 13C chemical shifts of recombinant rat ferrocytochrome b5, B conformation
  Swiss-Prot: P51608 released on 1996-10-01
    Title MECP2_HUMAN Entity Methyl-CpG-binding protein 2
  Swiss-Prot: Q00566 released on 1993-04-01
    Title MECP2_RAT Entity Methyl-CpG-binding protein 2

Related entities 1. chicken MeCP2/ARBP monomer, : 1 : 18 entities Detail

Interaction partners 1. chicken MeCP2/ARBP monomer, : 2 interactors Detail

More details for 2 interactors identified by 1 citations

Experiments performed 7 experiments Detail

1 HNCA

Instrument

#1 Model Bruker DRX (800 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Methyl-CpG-binding protein, attachment region binding protein	[U-15N; U-13C]		2.0 mM

Sample #2

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Methyl-CpG-binding protein, attachment region binding protein	[U-15N]		2.0 mM

Sample #3

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Methyl-CpG-binding protein, attachment region binding protein			2.0 mM

Sample #4

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	Methyl-CpG-binding protein, attachment region binding protein	[U-2H; U-13C; U-15N]		2.0 mM

2 CBCA(CO)NH

Instrument

#1 Model Bruker DRX (800 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Methyl-CpG-binding protein, attachment region binding protein	[U-15N; U-13C]		2.0 mM

Sample #2

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Methyl-CpG-binding protein, attachment region binding protein	[U-15N]		2.0 mM

Sample #3

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Methyl-CpG-binding protein, attachment region binding protein			2.0 mM

Sample #4

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	Methyl-CpG-binding protein, attachment region binding protein	[U-2H; U-13C; U-15N]		2.0 mM

3 1H-15N-HSQC

Instrument

#1 Model Bruker DRX (800 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Methyl-CpG-binding protein, attachment region binding protein	[U-15N; U-13C]		2.0 mM

Sample #2

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Methyl-CpG-binding protein, attachment region binding protein	[U-15N]		2.0 mM

Sample #3

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Methyl-CpG-binding protein, attachment region binding protein			2.0 mM

Sample #4

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	Methyl-CpG-binding protein, attachment region binding protein	[U-2H; U-13C; U-15N]		2.0 mM

4 1H-15N-NOESY HSQC

Instrument

#1 Model Bruker DRX (800 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Methyl-CpG-binding protein, attachment region binding protein	[U-15N; U-13C]		2.0 mM

Sample #2

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Methyl-CpG-binding protein, attachment region binding protein	[U-15N]		2.0 mM

Sample #3

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Methyl-CpG-binding protein, attachment region binding protein			2.0 mM

Sample #4

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	Methyl-CpG-binding protein, attachment region binding protein	[U-2H; U-13C; U-15N]		2.0 mM

5 1H-15N-TOCSY HSQC

Instrument

#1 Model Bruker DRX (800 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Methyl-CpG-binding protein, attachment region binding protein	[U-15N; U-13C]		2.0 mM

Sample #2

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Methyl-CpG-binding protein, attachment region binding protein	[U-15N]		2.0 mM

Sample #3

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Methyl-CpG-binding protein, attachment region binding protein			2.0 mM

Sample #4

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	Methyl-CpG-binding protein, attachment region binding protein	[U-2H; U-13C; U-15N]		2.0 mM

6 HNCO

Instrument

#1 Model Bruker DRX (800 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Methyl-CpG-binding protein, attachment region binding protein	[U-15N; U-13C]		2.0 mM

Sample #2

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Methyl-CpG-binding protein, attachment region binding protein	[U-15N]		2.0 mM

Sample #3

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Methyl-CpG-binding protein, attachment region binding protein			2.0 mM

Sample #4

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	Methyl-CpG-binding protein, attachment region binding protein	[U-2H; U-13C; U-15N]		2.0 mM

7 HCCH-TOCSY

Instrument

#1 Model Bruker DRX (800 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Methyl-CpG-binding protein, attachment region binding protein	[U-15N; U-13C]		2.0 mM

Sample #2

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Methyl-CpG-binding protein, attachment region binding protein	[U-15N]		2.0 mM

Sample #3

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Methyl-CpG-binding protein, attachment region binding protein			2.0 mM

Sample #4

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	Methyl-CpG-binding protein, attachment region binding protein	[U-2H; U-13C; U-15N]		2.0 mM

nullKeywords Chicken methyl-CpG-binding protein 2 (cMeCP2/ARBP), NMR assignments

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Found 1 Document (1.759 seconds)

BMRB: 4467

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles BMRB: 5, Swiss-Prot: 2 entries Detail

Related entities 1. chicken MeCP2/ARBP monomer, : 1 : 18 entities Detail

Interaction partners 1. chicken MeCP2/ARBP monomer, : 2 interactors Detail

Experiments performed 7 experiments Detail

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