BMRBj - BMREntryMaster

Found 1 Document (2.081 seconds)

BMRB: 4526

1DC2

SOLUTION NMR STRUCTURE OF TUMOR SUPPRESSOR P16INK4A, 20 STRUCTURES

Authors

Yuan, C., Selby, T.L., Li, J., Byeon, I.J.L., Tsai, M.D.

Assembly

CYCLIN-DEPENDENT KINASE 4 INHIBITOR A (P16INK4A)

Entity

1. CYCLIN-DEPENDENT KINASE 4 INHIBITOR A (P16INK4A) (polymer, Thiol state: all free), 156 monomers, 16532.45 Da Detail

MEPAAGSSME PSADWLATAA ARGRVEEVRA LLEAGALPNA PNSYGRRPIQ VMMMGSARVA ELLLLHGAEP NCADPATLTR PVHDAAREGF LDTLVVLHRA GARLDVRDAW GRLPVDLAEE LGHRDVARYL RAAAGGTRGS NHARIDAAEG PSDIPD

Formula weight

16532.45 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 87.2 %, Completeness: 40.6 %, Completeness (bb): 56.2 % Detail

Polymer type: polypeptide(L)

	Total	¹³C
All	40.6 % (264 of 650)	40.6 % (264 of 650)
Backbone	56.2 % (255 of 454)	56.2 % (255 of 454)
Sidechain	34.6 % (117 of 338)	34.6 % (117 of 338)
Aromatic	0.0 % (0 of 33)	0.0 % (0 of 33)
Methyl	33.0 % (31 of 94)	33.0 % (31 of 94)

1. CYCLIN-DEPENDENT KINASE 4 INHIBITOR A (P16INK4A)

MEPAAGSSME PSADWLATAA ARGRVEEVRA LLEAGALPNA PNSYGRRPIQ VMMMGSARVA ELLLLHGAEP NCADPATLTR PVHDAAREGF LDTLVVLHRA GARLDVRDAW GRLPVDLAEE LGHRDVARYL RAAAGGTRGS NHARIDAAEG PSDIPD

Show sample condition

Sample #1

Pressure 1 atm, Temperature 293 K, pH 7.5

#	Name	Isotope labeling	Concentration
1	CYCLIN-DEPENDENT KINASE 4 INHIBITOR A (P16INK4A)	[U-15N; U-13C]	0.2 ~ 0.4 mM
2	HEPES		4 mM
3	DTT		1 mM
4	EDTA		5 uM
5	H2O		95 %
6	D2O		5 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 7.5

#	Name	Isotope labeling	Concentration
7	CYCLIN-DEPENDENT KINASE 4 INHIBITOR A (P16INK4A)	[U-15N]	0.2 ~ 0.4 mM
8	HEPES		4 mM
9	DTT		1 mM
10	EDTA		5 uM
11	H2O		95 %
12	D2O		5 %

Sample #3

Pressure 1 atm, Temperature 293 K, pH 7.5

#	Name	Concentration
13	CYCLIN-DEPENDENT KINASE 4 INHIBITOR A (P16INK4A)	0.2 ~ 0.4 mM
14	HEPES	4 mM
15	DTT	1 mM
16	EDTA	5 uM
17	H2O	95 %
18	D2O	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	na	na	0.0 ppm	null	null	0.0

Referencing offset: -0.08 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	na	na	0.0 ppm	null	null	0.0

Referencing offset: -0.08 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1DC2, Strand ID: A Detail

Release date

2001-03-07

Citation

Tumor suppressor INK4: refinement of p16INK4A structure and determination of p15INK4B structure by comparative modeling and NMR data
Yuan, C., Selby, T.L., Li, J., Byeon, I.J.L., Tsai, M.D.
Protein Sci. (2000), 9, 1120-1128, PubMed 10892805 , DOI 10.1110/ps.9.6.1120 , Abstract

Entries sharing articles Swiss-Prot: 2 entries Detail

  Swiss-Prot: P42771 released on 1995-11-01
    Title CD2A1_HUMAN Entity Cyclin-dependent kinase inhibitor 2A, isoforms 1/2/3
  Swiss-Prot: P42772 released on 1995-11-01
    Title CDN2B_HUMAN Entity Cyclin-dependent kinase 4 inhibitor B

Related entities 1. CYCLIN-DEPENDENT KINASE 4 INHIBITOR A (P16INK4A), : 1 : 5 : 1 : 100 entities Detail

Interaction partners 1. CYCLIN-DEPENDENT KINASE 4 INHIBITOR A (P16INK4A), : 47 interactors Detail

More details for 47 interactors identified by 11 citations

Experiments performed 6 experiments Detail

1 3D_13C-SEPARATED NOESY

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 7.5

#	Name	Isotope labeling	Concentration
1	CYCLIN-DEPENDENT KINASE 4 INHIBITOR A (P16INK4A)	[U-15N; U-13C]	0.2 ~ 0.4 mM
2	HEPES		4 mM
3	DTT		1 mM
4	EDTA		5 uM
5	H2O		95 %
6	D2O		5 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 7.5

#	Name	Isotope labeling	Concentration
7	CYCLIN-DEPENDENT KINASE 4 INHIBITOR A (P16INK4A)	[U-15N]	0.2 ~ 0.4 mM
8	HEPES		4 mM
9	DTT		1 mM
10	EDTA		5 uM
11	H2O		95 %
12	D2O		5 %

Sample #3

Pressure 1 atm, Temperature 293 K, pH 7.5

#	Name	Concentration
13	CYCLIN-DEPENDENT KINASE 4 INHIBITOR A (P16INK4A)	0.2 ~ 0.4 mM
14	HEPES	4 mM
15	DTT	1 mM
16	EDTA	5 uM
17	H2O	95 %
18	D2O	5 %

2 4D_13C-SEPARATED NOESY

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 7.5

#	Name	Isotope labeling	Concentration
1	CYCLIN-DEPENDENT KINASE 4 INHIBITOR A (P16INK4A)	[U-15N; U-13C]	0.2 ~ 0.4 mM
2	HEPES		4 mM
3	DTT		1 mM
4	EDTA		5 uM
5	H2O		95 %
6	D2O		5 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 7.5

#	Name	Isotope labeling	Concentration
7	CYCLIN-DEPENDENT KINASE 4 INHIBITOR A (P16INK4A)	[U-15N]	0.2 ~ 0.4 mM
8	HEPES		4 mM
9	DTT		1 mM
10	EDTA		5 uM
11	H2O		95 %
12	D2O		5 %

Sample #3

Pressure 1 atm, Temperature 293 K, pH 7.5

#	Name	Concentration
13	CYCLIN-DEPENDENT KINASE 4 INHIBITOR A (P16INK4A)	0.2 ~ 0.4 mM
14	HEPES	4 mM
15	DTT	1 mM
16	EDTA	5 uM
17	H2O	95 %
18	D2O	5 %

3 4D_13C/15N-SEPARATED NOESY

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 7.5

#	Name	Isotope labeling	Concentration
1	CYCLIN-DEPENDENT KINASE 4 INHIBITOR A (P16INK4A)	[U-15N; U-13C]	0.2 ~ 0.4 mM
2	HEPES		4 mM
3	DTT		1 mM
4	EDTA		5 uM
5	H2O		95 %
6	D2O		5 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 7.5

#	Name	Isotope labeling	Concentration
7	CYCLIN-DEPENDENT KINASE 4 INHIBITOR A (P16INK4A)	[U-15N]	0.2 ~ 0.4 mM
8	HEPES		4 mM
9	DTT		1 mM
10	EDTA		5 uM
11	H2O		95 %
12	D2O		5 %

Sample #3

Pressure 1 atm, Temperature 293 K, pH 7.5

#	Name	Concentration
13	CYCLIN-DEPENDENT KINASE 4 INHIBITOR A (P16INK4A)	0.2 ~ 0.4 mM
14	HEPES	4 mM
15	DTT	1 mM
16	EDTA	5 uM
17	H2O	95 %
18	D2O	5 %

4 3D_15N-SEPARATED NOESY

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 7.5

#	Name	Isotope labeling	Concentration
1	CYCLIN-DEPENDENT KINASE 4 INHIBITOR A (P16INK4A)	[U-15N; U-13C]	0.2 ~ 0.4 mM
2	HEPES		4 mM
3	DTT		1 mM
4	EDTA		5 uM
5	H2O		95 %
6	D2O		5 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 7.5

#	Name	Isotope labeling	Concentration
7	CYCLIN-DEPENDENT KINASE 4 INHIBITOR A (P16INK4A)	[U-15N]	0.2 ~ 0.4 mM
8	HEPES		4 mM
9	DTT		1 mM
10	EDTA		5 uM
11	H2O		95 %
12	D2O		5 %

Sample #3

Pressure 1 atm, Temperature 293 K, pH 7.5

#	Name	Concentration
13	CYCLIN-DEPENDENT KINASE 4 INHIBITOR A (P16INK4A)	0.2 ~ 0.4 mM
14	HEPES	4 mM
15	DTT	1 mM
16	EDTA	5 uM
17	H2O	95 %
18	D2O	5 %

5 HNHA

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 7.5

#	Name	Isotope labeling	Concentration
1	CYCLIN-DEPENDENT KINASE 4 INHIBITOR A (P16INK4A)	[U-15N; U-13C]	0.2 ~ 0.4 mM
2	HEPES		4 mM
3	DTT		1 mM
4	EDTA		5 uM
5	H2O		95 %
6	D2O		5 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 7.5

#	Name	Isotope labeling	Concentration
7	CYCLIN-DEPENDENT KINASE 4 INHIBITOR A (P16INK4A)	[U-15N]	0.2 ~ 0.4 mM
8	HEPES		4 mM
9	DTT		1 mM
10	EDTA		5 uM
11	H2O		95 %
12	D2O		5 %

Sample #3

Pressure 1 atm, Temperature 293 K, pH 7.5

#	Name	Concentration
13	CYCLIN-DEPENDENT KINASE 4 INHIBITOR A (P16INK4A)	0.2 ~ 0.4 mM
14	HEPES	4 mM
15	DTT	1 mM
16	EDTA	5 uM
17	H2O	95 %
18	D2O	5 %

6 2D NOESY

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 7.5

#	Name	Isotope labeling	Concentration
1	CYCLIN-DEPENDENT KINASE 4 INHIBITOR A (P16INK4A)	[U-15N; U-13C]	0.2 ~ 0.4 mM
2	HEPES		4 mM
3	DTT		1 mM
4	EDTA		5 uM
5	H2O		95 %
6	D2O		5 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 7.5

#	Name	Isotope labeling	Concentration
7	CYCLIN-DEPENDENT KINASE 4 INHIBITOR A (P16INK4A)	[U-15N]	0.2 ~ 0.4 mM
8	HEPES		4 mM
9	DTT		1 mM
10	EDTA		5 uM
11	H2O		95 %
12	D2O		5 %

Sample #3

Pressure 1 atm, Temperature 293 K, pH 7.5

#	Name	Concentration
13	CYCLIN-DEPENDENT KINASE 4 INHIBITOR A (P16INK4A)	0.2 ~ 0.4 mM
14	HEPES	4 mM
15	DTT	1 mM
16	EDTA	5 uM
17	H2O	95 %
18	D2O	5 %

nullKeywords ANKYRIN REPEAT, HELIX-TURN-HELIX, HELIX BUNDLE

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Found 1 Document (2.081 seconds)

BMRB: 4526

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Chemical shift reference

Chemical shift reference

Entries sharing articles Swiss-Prot: 2 entries Detail

Related entities 1. CYCLIN-DEPENDENT KINASE 4 INHIBITOR A (P16INK4A), : 1 : 5 : 1 : 100 entities Detail

Interaction partners 1. CYCLIN-DEPENDENT KINASE 4 INHIBITOR A (P16INK4A), : 47 interactors Detail

Experiments performed 6 experiments Detail

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