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1H and 13C chemical shift assignments of native hen egg white lysozyme
Authors
Wang, Y., Bjorndahl, T.C., Wishart, D.S.
Assembly
Hen Egg White Lysozyme
Entity
1. Hen Egg White Lysozyme (polymer, Thiol state: all disulfide bound), 129 monomers, 14313.01 Da Detail

KVFGRCELAA AMKRHGLDNY RGYSLGNWVC AAKFESNFNT QATNRNTDGS TDYGILQINS RWWCNDGRTP GSRNLCNIPC SALLSSDITA SVNCAKKIVS DGNGMNAWVA WRNRCKGTDV QAWIRGCRL


Formula weight
14313.01 Da
Source organism
Gallus gallus
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 85.8 %, Completeness (bb): 80.3 % Detail

Polymer type: polypeptide(L)

Total1H13C
All85.8 % (1116 of 1300)92.8 % (698 of 752)76.3 % (418 of 548)
Backbone80.3 % (507 of 631)98.4 % (252 of 256)68.0 % (255 of 375)
Sidechain92.1 % (724 of 786)89.9 % (446 of 496)95.9 % (278 of 290)
Aromatic97.6 % (121 of 124)96.9 % (63 of 65)98.3 % (58 of 59)
Methyl99.2 % (117 of 118)100.0 % (59 of 59)98.3 % (58 of 59)

1. hen egg white lysozyme

KVFGRCELAA AMKRHGLDNY RGYSLGNWVC AAKFESNFNT QATNRNTDGS TDYGILQINS RWWCNDGRTP GSRNLCNIPC SALLSSDITA SVNCAKKIVS DGNGMNAWVA WRNRCKGTDV QAWIRGCRL

Sample

Temperature 318 K, pH 3.6


#NameIsotope labelingTypeConcentration
1hen egg white lysozyme10 mM

LACS Plot; CA
Referencing offset: -0.08 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: -0.08 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: -0.06 ppm, Outliers: 1 Detail
Release date
2000-01-11
Citation
Complete 1H and non-carbonylic 13C assignments of native hen egg-white lysozyme
Wang, Y., Bjorndahl, T.C., Wishart, D.S.
J. Biomol. NMR (2000), 17, 83-84, PubMed 10909869 ,
Related entities 1. Hen Egg White Lysozyme, : 1 : 103 : 1 : 98 entities Detail
Interaction partners 1. Hen Egg White Lysozyme, : 8 interactors Detail