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Native and non-native secondary structure and dynamics in the pH 4 intermediate of apomyoglobin
Authors
Eliezer, D., Chung, J., Dyson, J., Wright, P.E.
Assembly
apomyoglobin
Entity
1. apomyoglobin (polymer, Thiol state: not present), 153 monomers, 17199.65 Da Detail

VLSEGEWQLV LHVWAKVEAD VAGHGQDILI RLFKSHPETL EKFDRFKHLK TEAEMKASED LKKHGVTVLT ALGAILKKKG HHEAELKPLA QSHATKHKIP IKYLEFISEA IIHVLHSRHP GDFGADAQGA MNKALELFRK DIAAKYKELG YQG


Formula weight
17199.65 Da
Source organism
Physeter catodon
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 47.8 %, Completeness (bb): 95.0 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All47.8 % (877 of 1836)31.0 % (295 of 953)60.0 % (433 of 722)92.5 % (149 of 161)
Backbone95.0 % (854 of 899)91.7 % (277 of 302)95.5 % (428 of 448)100.0 % (149 of 149)
Sidechain14.1 % (152 of 1079) 2.8 % (18 of 651)32.2 % (134 of 416) 0.0 % (0 of 12)
Aromatic 2.6 % (4 of 156) 2.6 % (2 of 78) 2.6 % (2 of 76) 0.0 % (0 of 2)
Methyl12.0 % (22 of 184) 2.2 % (2 of 92)21.7 % (20 of 92)

1. myoglobin

VLSEGEWQLV LHVWAKVEAD VAGHGQDILI RLFKSHPETL EKFDRFKHLK TEAEMKASED LKKHGVTVLT ALGAILKKKG HHEAELKPLA QSHATKHKIP IKYLEFISEA IIHVLHSRHP GDFGADAQGA MNKALELFRK DIAAKYKELG YQG

Sample

Temperature 323 (±1) K, pH 4.1 (±0.1)


#NameIsotope labelingTypeConcentration
1myoglobin[U-95% 13C; U-90% 15N; U-80% 2H]0.3 mM
2sodium acetate[U-90% 2H]10 mM
3ethanol[U-90% 2H]10 % v/v

LACS Plot; CA
Referencing offset: -0.12 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: -0.12 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: 0.06 ppm, Outliers: 2 Detail
LACS Plot; CO
Referencing offset: 0.31 ppm, Outliers: 7 Detail
Release date
2000-05-24
Citation
Native and non-native secondary structure and dynamics in the pH 4 intermediate of apomyoglobin
Eliezer, D., Chung, J., Dyson, J., Wright, P.E.
Biochemistry
Related entities 1. apomyoglobin, : 1 : 93 : 7 : 101 entities Detail
Interaction partners 1. apomyoglobin, : 2 interactors Detail
Experiments performed 7 experiments Detail
Keywords equilibrium folding intermediate