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BMRB: 4577

1C05

1H, 13C, and 15N Chemical Shift Assignments for Ribosomal Protein S4 delta 41

Authors

Markus, M.A., Gerstner, R.B., Draper, D.E., Torchia, D.A.

Assembly

RIBOSOMAL PROTEIN S4 DELTA 41

Entity

1. RIBOSOMAL PROTEIN S4 DELTA 41 (polymer, Thiol state: not present), 159 monomers, 18594.10 Da Detail

MKLSEYGLQL QEKQKLRHMY GVNERQFRKT FEEAGKMPGK HGENFMILLE SRLDNLVYRL GLARTRRQAR QLVTHGHILV DGSRVNIPSY RVKPGQTIAV REKSRNLQVI KEALEANNYI PDYLSFDPEK MEGTYTRLPE RSELPAEINE ALIVEFYSR

Formula weight

18594.1 Da

Source organism

Geobacillus stearothermophilus

Exptl. method

NMR

Refine. method

hybrid DGSA protocol without the dipolar coupling restraints followed by additional SA with the dipolar coupling restraints add

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 91.8 %, Completeness (bb): 98.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	91.8 % (1781 of 1941)	88.9 % (914 of 1028)	94.0 % (700 of 745)	99.4 % (167 of 168)
Backbone	98.3 % (924 of 940)	98.4 % (316 of 321)	97.9 % (457 of 467)	99.3 % (151 of 152)
Sidechain	87.4 % (1005 of 1150)	84.6 % (598 of 707)	91.6 % (391 of 427)	100.0 % (16 of 16)
Aromatic	61.5 % (80 of 130)	63.1 % (41 of 65)	60.0 % (39 of 65)
Methyl	96.4 % (162 of 168)	96.4 % (81 of 84)	96.4 % (81 of 84)

1. RIBOSOMAL PROTEIN S4 DELTA 41

MKLSEYGLQL QEKQKLRHMY GVNERQFRKT FEEAGKMPGK HGENFMILLE SRLDNLVYRL GLARTRRQAR QLVTHGHILV DGSRVNIPSY RVKPGQTIAV REKSRNLQVI KEALEANNYI PDYLSFDPEK MEGTYTRLPE RSELPAEINE ALIVEFYSR

Show sample condition

Sample #1

Pressure 1 atm, Temperature 310 (±0.5) K, pH 5.4 (±0.1)

#	Name	Isotope labeling	Concentration
1	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N]	0.800 mM
2	deuterated acetate		20 mM
3	KCl		250 mM
4	sodium azide		0.1 mM

Sample #2

Pressure 1 atm, Temperature 310 (±0.5) K, pH 5.4 (±0.1)

#	Name	Isotope labeling	Concentration
5	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N; U-13C]	1.1 mM
6	D2O		6 %
7	deuterated acetate		20 mM
8	KCl		250 mM
9	sodium azide		0.1 mM

Sample #3

Pressure 1 atm, Temperature 310 (±0.5) K, pH 5.4 (±0.1)

#	Name	Isotope labeling	Concentration
10	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N; U-13C]	1.1 mM
11	D2O		99.9 %
12	deuterated acetate		20 mM
13	KCl		250 mM
14	sodium azide		0.1 mM

Sample #4

Pressure 1 atm, Temperature 310 (±0.5) K, pH 5.4 (±0.1)

#	Name	Isotope labeling	Concentration
15	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N]	0.100 mM
16	3:1 DMPC:DHPC		0.0 ~ 0.0 na
17	KH2PO4		10 mM
18	sodium azide		0.1 mM
19	D2O		7 %

Sample #5

Pressure 1 atm, Temperature 310 (±0.5) K, pH 5.4 (±0.1), Details residue specific labelling as follows: proline: deuterated at beta, gamma, and delta positions; methionine: uniformly 15N and 13C labelled (also protonated); tyrosine: 13C labelled at the epsilon position of the ring (also protonated)

#	Name	Isotope labeling	Concentration
20	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N; U-85% 2H], [U-13C]-MET, [13C, CE]-TYR	0.214 mM
21	Pf1 phage		2.5 mg/mL
22	KH2PO4		10 mM
23	KCl		300 mM
24	sodium azide		0.2 mM
25	D2O		6 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	TSP	methyl protons	0.0 ppm	external	direct	0.0
¹³C	TSP	methyl protons	0.0 ppm	null	indirect	0.2514495
¹⁵N	liquid ammonia	nitrogen	0.0 na	external	indirect	0.0

Referencing offset: -0.26 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	TSP	methyl protons	0.0 ppm	external	direct	0.0
¹³C	TSP	methyl protons	0.0 ppm	null	indirect	0.2514495
¹⁵N	liquid ammonia	nitrogen	0.0 na	external	indirect	0.0

Referencing offset: -0.26 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	TSP	methyl protons	0.0 ppm	external	direct	0.0
¹³C	TSP	methyl protons	0.0 ppm	null	indirect	0.2514495
¹⁵N	liquid ammonia	nitrogen	0.0 na	external	indirect	0.0

Referencing offset: -0.04 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	TSP	methyl protons	0.0 ppm	external	direct	0.0
¹³C	TSP	methyl protons	0.0 ppm	null	indirect	0.2514495
¹⁵N	liquid ammonia	nitrogen	0.0 na	external	indirect	0.0

Referencing offset: -0.15 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 1 models in PDB: 1C05, Strand ID: A Detail

Coupling constant

113 J values in 1 lists
Pressure 1 atm, Temperature 310 (±0.5) K, pH 5.4 (±0.1) Detail

Release date

2000-03-05

Citation 1

The solution structure of ribosomal protein S4 delta41 reveals two subdomains and a positively charged surface that may interact with RNA
Markus, M.A., Gerstner, R.B., Draper, D.E., Torchia, D.A.
EMBO J. (1998), 17, 4559-4571, PubMed , DOI:

Show more 1 citaion

Citation 2

Refining the overall structure and subdomain orientation of ribosomal protein S4 delta41 with dipolar couplings measured by NMR in uniaxial liquid crystalline phases
Markus, M.A., Gerstner, R.B., Draper, D.E., Torchia, D.A.
J. Mol. Biol. (1999), 292, 375-387, PubMed 10493882 , DOI 10.1006/jmbi.1999.3061 , Abstract

Hide non-primary 1 citaion

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: P81288 released on 1998-12-15
Title RS4_GEOSE Entity 30S ribosomal protein S4

Related entities 1. RIBOSOMAL PROTEIN S4 DELTA 41, : 1 : 3 : 225 entities Detail

Interaction partners 1. RIBOSOMAL PROTEIN S4 DELTA 41, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 16 experiments Detail

1 HNCACB

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (750 MHz)

Sample #1

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
1	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N]	0.800 mM
2	deuterated acetate		20 mM
3	KCl		250 mM
4	sodium azide		0.1 mM

Sample #2

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
5	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N; U-13C]	1.1 mM
6	D2O		6 %
7	deuterated acetate		20 mM
8	KCl		250 mM
9	sodium azide		0.1 mM

Sample #3

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
10	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N; U-13C]	1.1 mM
11	D2O		99.9 %
12	deuterated acetate		20 mM
13	KCl		250 mM
14	sodium azide		0.1 mM

Sample #4

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
15	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N]	0.100 mM
16	3:1 DMPC:DHPC		0.0 ~ 0.0 na
17	KH2PO4		10 mM
18	sodium azide		0.1 mM
19	D2O		7 %

Sample #5

Pressure 1 atm, Temperature 310 (±0.5) K, Details residue specific labelling as follows: proline: deuterated at beta, gamma, and delta positions; methionine: uniformly 15N and 13C labelled (also protonated); tyrosine: 13C labelled at the epsilon position of the ring (also protonated)

#	Name	Isotope labeling	Concentration
20	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N; U-85% 2H], [U-13C]-MET, [13C, CE]-TYR	0.214 mM
21	Pf1 phage		2.5 mg/mL
22	KH2PO4		10 mM
23	KCl		300 mM
24	sodium azide		0.2 mM
25	D2O		6 %

2 CBCA(CO)NH

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (750 MHz)

Sample #1

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
1	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N]	0.800 mM
2	deuterated acetate		20 mM
3	KCl		250 mM
4	sodium azide		0.1 mM

Sample #2

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
5	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N; U-13C]	1.1 mM
6	D2O		6 %
7	deuterated acetate		20 mM
8	KCl		250 mM
9	sodium azide		0.1 mM

Sample #3

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
10	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N; U-13C]	1.1 mM
11	D2O		99.9 %
12	deuterated acetate		20 mM
13	KCl		250 mM
14	sodium azide		0.1 mM

Sample #4

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
15	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N]	0.100 mM
16	3:1 DMPC:DHPC		0.0 ~ 0.0 na
17	KH2PO4		10 mM
18	sodium azide		0.1 mM
19	D2O		7 %

Sample #5

#	Name	Isotope labeling	Concentration
20	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N; U-85% 2H], [U-13C]-MET, [13C, CE]-TYR	0.214 mM
21	Pf1 phage		2.5 mg/mL
22	KH2PO4		10 mM
23	KCl		300 mM
24	sodium azide		0.2 mM
25	D2O		6 %

3 HNCA

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (750 MHz)

Sample #1

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
1	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N]	0.800 mM
2	deuterated acetate		20 mM
3	KCl		250 mM
4	sodium azide		0.1 mM

Sample #2

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
5	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N; U-13C]	1.1 mM
6	D2O		6 %
7	deuterated acetate		20 mM
8	KCl		250 mM
9	sodium azide		0.1 mM

Sample #3

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
10	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N; U-13C]	1.1 mM
11	D2O		99.9 %
12	deuterated acetate		20 mM
13	KCl		250 mM
14	sodium azide		0.1 mM

Sample #4

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
15	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N]	0.100 mM
16	3:1 DMPC:DHPC		0.0 ~ 0.0 na
17	KH2PO4		10 mM
18	sodium azide		0.1 mM
19	D2O		7 %

Sample #5

#	Name	Isotope labeling	Concentration
20	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N; U-85% 2H], [U-13C]-MET, [13C, CE]-TYR	0.214 mM
21	Pf1 phage		2.5 mg/mL
22	KH2PO4		10 mM
23	KCl		300 mM
24	sodium azide		0.2 mM
25	D2O		6 %

4 HCACO

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (750 MHz)

Sample #1

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
1	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N]	0.800 mM
2	deuterated acetate		20 mM
3	KCl		250 mM
4	sodium azide		0.1 mM

Sample #2

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
5	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N; U-13C]	1.1 mM
6	D2O		6 %
7	deuterated acetate		20 mM
8	KCl		250 mM
9	sodium azide		0.1 mM

Sample #3

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
10	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N; U-13C]	1.1 mM
11	D2O		99.9 %
12	deuterated acetate		20 mM
13	KCl		250 mM
14	sodium azide		0.1 mM

Sample #4

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
15	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N]	0.100 mM
16	3:1 DMPC:DHPC		0.0 ~ 0.0 na
17	KH2PO4		10 mM
18	sodium azide		0.1 mM
19	D2O		7 %

Sample #5

#	Name	Isotope labeling	Concentration
20	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N; U-85% 2H], [U-13C]-MET, [13C, CE]-TYR	0.214 mM
21	Pf1 phage		2.5 mg/mL
22	KH2PO4		10 mM
23	KCl		300 mM
24	sodium azide		0.2 mM
25	D2O		6 %

5 HNCO

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (750 MHz)

Sample #1

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
1	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N]	0.800 mM
2	deuterated acetate		20 mM
3	KCl		250 mM
4	sodium azide		0.1 mM

Sample #2

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
5	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N; U-13C]	1.1 mM
6	D2O		6 %
7	deuterated acetate		20 mM
8	KCl		250 mM
9	sodium azide		0.1 mM

Sample #3

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
10	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N; U-13C]	1.1 mM
11	D2O		99.9 %
12	deuterated acetate		20 mM
13	KCl		250 mM
14	sodium azide		0.1 mM

Sample #4

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
15	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N]	0.100 mM
16	3:1 DMPC:DHPC		0.0 ~ 0.0 na
17	KH2PO4		10 mM
18	sodium azide		0.1 mM
19	D2O		7 %

Sample #5

#	Name	Isotope labeling	Concentration
20	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N; U-85% 2H], [U-13C]-MET, [13C, CE]-TYR	0.214 mM
21	Pf1 phage		2.5 mg/mL
22	KH2PO4		10 mM
23	KCl		300 mM
24	sodium azide		0.2 mM
25	D2O		6 %

6 HBHA(CBCACO)NH

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (750 MHz)

Sample #1

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
1	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N]	0.800 mM
2	deuterated acetate		20 mM
3	KCl		250 mM
4	sodium azide		0.1 mM

Sample #2

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
5	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N; U-13C]	1.1 mM
6	D2O		6 %
7	deuterated acetate		20 mM
8	KCl		250 mM
9	sodium azide		0.1 mM

Sample #3

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
10	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N; U-13C]	1.1 mM
11	D2O		99.9 %
12	deuterated acetate		20 mM
13	KCl		250 mM
14	sodium azide		0.1 mM

Sample #4

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
15	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N]	0.100 mM
16	3:1 DMPC:DHPC		0.0 ~ 0.0 na
17	KH2PO4		10 mM
18	sodium azide		0.1 mM
19	D2O		7 %

Sample #5

#	Name	Isotope labeling	Concentration
20	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N; U-85% 2H], [U-13C]-MET, [13C, CE]-TYR	0.214 mM
21	Pf1 phage		2.5 mg/mL
22	KH2PO4		10 mM
23	KCl		300 mM
24	sodium azide		0.2 mM
25	D2O		6 %

7 HNHB

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (750 MHz)

Sample #1

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
1	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N]	0.800 mM
2	deuterated acetate		20 mM
3	KCl		250 mM
4	sodium azide		0.1 mM

Sample #2

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
5	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N; U-13C]	1.1 mM
6	D2O		6 %
7	deuterated acetate		20 mM
8	KCl		250 mM
9	sodium azide		0.1 mM

Sample #3

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
10	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N; U-13C]	1.1 mM
11	D2O		99.9 %
12	deuterated acetate		20 mM
13	KCl		250 mM
14	sodium azide		0.1 mM

Sample #4

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
15	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N]	0.100 mM
16	3:1 DMPC:DHPC		0.0 ~ 0.0 na
17	KH2PO4		10 mM
18	sodium azide		0.1 mM
19	D2O		7 %

Sample #5

#	Name	Isotope labeling	Concentration
20	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N; U-85% 2H], [U-13C]-MET, [13C, CE]-TYR	0.214 mM
21	Pf1 phage		2.5 mg/mL
22	KH2PO4		10 mM
23	KCl		300 mM
24	sodium azide		0.2 mM
25	D2O		6 %

8 C(CO)NH

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (750 MHz)

Sample #1

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
1	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N]	0.800 mM
2	deuterated acetate		20 mM
3	KCl		250 mM
4	sodium azide		0.1 mM

Sample #2

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
5	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N; U-13C]	1.1 mM
6	D2O		6 %
7	deuterated acetate		20 mM
8	KCl		250 mM
9	sodium azide		0.1 mM

Sample #3

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
10	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N; U-13C]	1.1 mM
11	D2O		99.9 %
12	deuterated acetate		20 mM
13	KCl		250 mM
14	sodium azide		0.1 mM

Sample #4

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
15	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N]	0.100 mM
16	3:1 DMPC:DHPC		0.0 ~ 0.0 na
17	KH2PO4		10 mM
18	sodium azide		0.1 mM
19	D2O		7 %

Sample #5

#	Name	Isotope labeling	Concentration
20	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N; U-85% 2H], [U-13C]-MET, [13C, CE]-TYR	0.214 mM
21	Pf1 phage		2.5 mg/mL
22	KH2PO4		10 mM
23	KCl		300 mM
24	sodium azide		0.2 mM
25	D2O		6 %

9 H(CCO)NH

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (750 MHz)

Sample #1

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
1	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N]	0.800 mM
2	deuterated acetate		20 mM
3	KCl		250 mM
4	sodium azide		0.1 mM

Sample #2

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
5	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N; U-13C]	1.1 mM
6	D2O		6 %
7	deuterated acetate		20 mM
8	KCl		250 mM
9	sodium azide		0.1 mM

Sample #3

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
10	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N; U-13C]	1.1 mM
11	D2O		99.9 %
12	deuterated acetate		20 mM
13	KCl		250 mM
14	sodium azide		0.1 mM

Sample #4

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
15	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N]	0.100 mM
16	3:1 DMPC:DHPC		0.0 ~ 0.0 na
17	KH2PO4		10 mM
18	sodium azide		0.1 mM
19	D2O		7 %

Sample #5

#	Name	Isotope labeling	Concentration
20	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N; U-85% 2H], [U-13C]-MET, [13C, CE]-TYR	0.214 mM
21	Pf1 phage		2.5 mg/mL
22	KH2PO4		10 mM
23	KCl		300 mM
24	sodium azide		0.2 mM
25	D2O		6 %

10 HCCH-TOCSY

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (750 MHz)

Sample #1

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
1	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N]	0.800 mM
2	deuterated acetate		20 mM
3	KCl		250 mM
4	sodium azide		0.1 mM

Sample #2

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
5	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N; U-13C]	1.1 mM
6	D2O		6 %
7	deuterated acetate		20 mM
8	KCl		250 mM
9	sodium azide		0.1 mM

Sample #3

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
10	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N; U-13C]	1.1 mM
11	D2O		99.9 %
12	deuterated acetate		20 mM
13	KCl		250 mM
14	sodium azide		0.1 mM

Sample #4

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
15	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N]	0.100 mM
16	3:1 DMPC:DHPC		0.0 ~ 0.0 na
17	KH2PO4		10 mM
18	sodium azide		0.1 mM
19	D2O		7 %

Sample #5

#	Name	Isotope labeling	Concentration
20	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N; U-85% 2H], [U-13C]-MET, [13C, CE]-TYR	0.214 mM
21	Pf1 phage		2.5 mg/mL
22	KH2PO4		10 mM
23	KCl		300 mM
24	sodium azide		0.2 mM
25	D2O		6 %

11 13C HSQC

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (750 MHz)

Sample #1

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
1	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N]	0.800 mM
2	deuterated acetate		20 mM
3	KCl		250 mM
4	sodium azide		0.1 mM

Sample #2

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
5	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N; U-13C]	1.1 mM
6	D2O		6 %
7	deuterated acetate		20 mM
8	KCl		250 mM
9	sodium azide		0.1 mM

Sample #3

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
10	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N; U-13C]	1.1 mM
11	D2O		99.9 %
12	deuterated acetate		20 mM
13	KCl		250 mM
14	sodium azide		0.1 mM

Sample #4

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
15	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N]	0.100 mM
16	3:1 DMPC:DHPC		0.0 ~ 0.0 na
17	KH2PO4		10 mM
18	sodium azide		0.1 mM
19	D2O		7 %

Sample #5

#	Name	Isotope labeling	Concentration
20	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N; U-85% 2H], [U-13C]-MET, [13C, CE]-TYR	0.214 mM
21	Pf1 phage		2.5 mg/mL
22	KH2PO4		10 mM
23	KCl		300 mM
24	sodium azide		0.2 mM
25	D2O		6 %

12 3D_15N-separated_NOESY

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (750 MHz)

Sample #1

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
1	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N]	0.800 mM
2	deuterated acetate		20 mM
3	KCl		250 mM
4	sodium azide		0.1 mM

Sample #2

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
5	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N; U-13C]	1.1 mM
6	D2O		6 %
7	deuterated acetate		20 mM
8	KCl		250 mM
9	sodium azide		0.1 mM

Sample #3

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
10	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N; U-13C]	1.1 mM
11	D2O		99.9 %
12	deuterated acetate		20 mM
13	KCl		250 mM
14	sodium azide		0.1 mM

Sample #4

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
15	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N]	0.100 mM
16	3:1 DMPC:DHPC		0.0 ~ 0.0 na
17	KH2PO4		10 mM
18	sodium azide		0.1 mM
19	D2O		7 %

Sample #5

#	Name	Isotope labeling	Concentration
20	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N; U-85% 2H], [U-13C]-MET, [13C, CE]-TYR	0.214 mM
21	Pf1 phage		2.5 mg/mL
22	KH2PO4		10 mM
23	KCl		300 mM
24	sodium azide		0.2 mM
25	D2O		6 %

13 3D_13C-separated_NOESY

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (750 MHz)

Sample #1

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
1	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N]	0.800 mM
2	deuterated acetate		20 mM
3	KCl		250 mM
4	sodium azide		0.1 mM

Sample #2

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
5	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N; U-13C]	1.1 mM
6	D2O		6 %
7	deuterated acetate		20 mM
8	KCl		250 mM
9	sodium azide		0.1 mM

Sample #3

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
10	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N; U-13C]	1.1 mM
11	D2O		99.9 %
12	deuterated acetate		20 mM
13	KCl		250 mM
14	sodium azide		0.1 mM

Sample #4

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
15	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N]	0.100 mM
16	3:1 DMPC:DHPC		0.0 ~ 0.0 na
17	KH2PO4		10 mM
18	sodium azide		0.1 mM
19	D2O		7 %

Sample #5

#	Name	Isotope labeling	Concentration
20	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N; U-85% 2H], [U-13C]-MET, [13C, CE]-TYR	0.214 mM
21	Pf1 phage		2.5 mg/mL
22	KH2PO4		10 mM
23	KCl		300 mM
24	sodium azide		0.2 mM
25	D2O		6 %

14 4D_13C-separated_NOESY

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (750 MHz)

Sample #1

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
1	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N]	0.800 mM
2	deuterated acetate		20 mM
3	KCl		250 mM
4	sodium azide		0.1 mM

Sample #2

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
5	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N; U-13C]	1.1 mM
6	D2O		6 %
7	deuterated acetate		20 mM
8	KCl		250 mM
9	sodium azide		0.1 mM

Sample #3

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
10	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N; U-13C]	1.1 mM
11	D2O		99.9 %
12	deuterated acetate		20 mM
13	KCl		250 mM
14	sodium azide		0.1 mM

Sample #4

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
15	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N]	0.100 mM
16	3:1 DMPC:DHPC		0.0 ~ 0.0 na
17	KH2PO4		10 mM
18	sodium azide		0.1 mM
19	D2O		7 %

Sample #5

#	Name	Isotope labeling	Concentration
20	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N; U-85% 2H], [U-13C]-MET, [13C, CE]-TYR	0.214 mM
21	Pf1 phage		2.5 mg/mL
22	KH2PO4		10 mM
23	KCl		300 mM
24	sodium azide		0.2 mM
25	D2O		6 %

15 HNHA

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (750 MHz)

Sample #1

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
1	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N]	0.800 mM
2	deuterated acetate		20 mM
3	KCl		250 mM
4	sodium azide		0.1 mM

Sample #2

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
5	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N; U-13C]	1.1 mM
6	D2O		6 %
7	deuterated acetate		20 mM
8	KCl		250 mM
9	sodium azide		0.1 mM

Sample #3

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
10	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N; U-13C]	1.1 mM
11	D2O		99.9 %
12	deuterated acetate		20 mM
13	KCl		250 mM
14	sodium azide		0.1 mM

Sample #4

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
15	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N]	0.100 mM
16	3:1 DMPC:DHPC		0.0 ~ 0.0 na
17	KH2PO4		10 mM
18	sodium azide		0.1 mM
19	D2O		7 %

Sample #5

#	Name	Isotope labeling	Concentration
20	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N; U-85% 2H], [U-13C]-MET, [13C, CE]-TYR	0.214 mM
21	Pf1 phage		2.5 mg/mL
22	KH2PO4		10 mM
23	KCl		300 mM
24	sodium azide		0.2 mM
25	D2O		6 %

16 IPAP 15N HSQC

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (750 MHz)

Sample #1

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
1	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N]	0.800 mM
2	deuterated acetate		20 mM
3	KCl		250 mM
4	sodium azide		0.1 mM

Sample #2

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
5	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N; U-13C]	1.1 mM
6	D2O		6 %
7	deuterated acetate		20 mM
8	KCl		250 mM
9	sodium azide		0.1 mM

Sample #3

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
10	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N; U-13C]	1.1 mM
11	D2O		99.9 %
12	deuterated acetate		20 mM
13	KCl		250 mM
14	sodium azide		0.1 mM

Sample #4

Pressure 1 atm, Temperature 310 (±0.5) K

#	Name	Isotope labeling	Concentration
15	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N]	0.100 mM
16	3:1 DMPC:DHPC		0.0 ~ 0.0 na
17	KH2PO4		10 mM
18	sodium azide		0.1 mM
19	D2O		7 %

Sample #5

#	Name	Isotope labeling	Concentration
20	RIBOSOMAL PROTEIN S4 DELTA 41	[U-15N; U-85% 2H], [U-13C]-MET, [13C, CE]-TYR	0.214 mM
21	Pf1 phage		2.5 mg/mL
22	KH2PO4		10 mM
23	KCl		300 mM
24	sodium azide		0.2 mM
25	D2O		6 %

null

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BMRB: 4577

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Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. RIBOSOMAL PROTEIN S4 DELTA 41, : 1 : 3 : 225 entities Detail

Interaction partners 1. RIBOSOMAL PROTEIN S4 DELTA 41, : 1 interactors Detail

Experiments performed 16 experiments Detail

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