BMRBj - BMREntryMaster

Found 1 Document (2.022 seconds)

BMRB: 4579

1HYJ

Sequence-specific 1H, 15N and 13C resonance assignments of the EEA1 FYVE domain.

Authors

Kutateladze, T.G., Overduin, M.

Assembly

FYVE domain of EEA1

Entity

1. Early Endosome autoantigen 1 (polymer, Thiol state: all other bound), 86 monomers, 9579.795 Da Detail

AVQELGRENQ SLQIKHTQAL NRKWAEDNEV QNCMACGKGF SVTVRRHHCR QCGNIFCAEC SAKNALTPSS KKPVRVCDAC FNDLQG

2. ZINC ION (non-polymer), 65.409 × 2 Da

Total weight

9710.613 Da

Max. entity weight

9579.795 Da

Entity Connection

metal coordination 8 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	metal coordination	sing	1:CYS33:SG	2:ZN1:ZN
2	metal coordination	sing	1:CYS36:SG	2:ZN1:ZN
3	metal coordination	sing	1:CYS57:SG	2:ZN1:ZN
4	metal coordination	sing	1:CYS60:SG	2:ZN1:ZN
5	metal coordination	sing	1:CYS49:SG	2:ZN1:ZN
6	metal coordination	sing	1:CYS52:SG	2:ZN1:ZN
7	metal coordination	sing	1:CYS77:SG	2:ZN1:ZN
8	metal coordination	sing	1:CYS80:SG	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.7 %, Completeness (bb): 99.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	97.7 % (952 of 974)	97.9 % (501 of 512)	97.2 % (353 of 363)	99.0 % (98 of 99)
Backbone	99.0 % (507 of 512)	99.4 % (174 of 175)	98.8 % (250 of 253)	98.8 % (83 of 84)
Sidechain	96.9 % (526 of 543)	97.0 % (327 of 337)	96.3 % (184 of 191)	100.0 % (15 of 15)
Aromatic	100.0 % (54 of 54)	100.0 % (27 of 27)	100.0 % (26 of 26)	100.0 % (1 of 1)
Methyl	97.3 % (72 of 74)	97.3 % (36 of 37)	97.3 % (36 of 37)

1. Early Endosome autoantigen 1

AVQELGRENQ SLQIKHTQAL NRKWAEDNEV QNCMACGKGF SVTVRRHHCR QCGNIFCAEC SAKNALTPSS KKPVRVCDAC FNDLQG

Show sample condition

Sample #1

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Early Endosome autoantigen 1			1.0 mM

Sample #2

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Early Endosome autoantigen 1	[U-15N]		1.0 mM

Sample #3

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Early Endosome autoantigen 1	[U-15N; U-13C]		1.0 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	proton	4.74 ppm	internal	direct	0.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Referencing offset: -0.13 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	proton	4.74 ppm	internal	direct	0.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Referencing offset: -0.13 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	proton	4.74 ppm	internal	direct	0.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Referencing offset: 0.0 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	proton	4.74 ppm	internal	direct	0.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Referencing offset: 0.15 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1HYJ, Strand ID: A Detail

Release date

2000-06-18

Citation

Sequence-specific 1H, 15N and 13C resonance assignments of the EEA1 FYVE domain
Kutateladze, T.G., Overduin, M.
J. Biomol. NMR (2000), 17, 89-90, PubMed 10909872 , DOI: , Abstract

Related entities 1. Early Endosome autoantigen 1, : 3 : 36 entities Detail

Interaction partners 1. Early Endosome autoantigen 1, : 48 interactors Detail

More details for 48 interactors identified by 31 citations

Experiments performed 16 experiments Detail

1 2D TOCSY

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Early Endosome autoantigen 1			1.0 mM

Sample #2

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Early Endosome autoantigen 1	[U-15N]		1.0 mM

Sample #3

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Early Endosome autoantigen 1	[U-15N; U-13C]		1.0 mM

2 2D COSY

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Early Endosome autoantigen 1			1.0 mM

Sample #2

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Early Endosome autoantigen 1	[U-15N]		1.0 mM

Sample #3

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Early Endosome autoantigen 1	[U-15N; U-13C]		1.0 mM

3 3D 1H-15N DIPSI-HSQC

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Early Endosome autoantigen 1			1.0 mM

Sample #2

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Early Endosome autoantigen 1	[U-15N]		1.0 mM

Sample #3

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Early Endosome autoantigen 1	[U-15N; U-13C]		1.0 mM

4 3D 1H-13C HCCH TOCSY

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Early Endosome autoantigen 1			1.0 mM

Sample #2

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Early Endosome autoantigen 1	[U-15N]		1.0 mM

Sample #3

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Early Endosome autoantigen 1	[U-15N; U-13C]		1.0 mM

5 2D 1H-15N HSQC

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Early Endosome autoantigen 1			1.0 mM

Sample #2

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Early Endosome autoantigen 1	[U-15N]		1.0 mM

Sample #3

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Early Endosome autoantigen 1	[U-15N; U-13C]		1.0 mM

6 2D 1H-13C HSQC

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Early Endosome autoantigen 1			1.0 mM

Sample #2

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Early Endosome autoantigen 1	[U-15N]		1.0 mM

Sample #3

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Early Endosome autoantigen 1	[U-15N; U-13C]		1.0 mM

7 2D HBCBCGCDHD TOCSY

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Early Endosome autoantigen 1			1.0 mM

Sample #2

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Early Endosome autoantigen 1	[U-15N]		1.0 mM

Sample #3

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Early Endosome autoantigen 1	[U-15N; U-13C]		1.0 mM

8 2D HBCBCGCDCEHE TOCSY

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Early Endosome autoantigen 1			1.0 mM

Sample #2

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Early Endosome autoantigen 1	[U-15N]		1.0 mM

Sample #3

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Early Endosome autoantigen 1	[U-15N; U-13C]		1.0 mM

9 3D HNCACB

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Early Endosome autoantigen 1			1.0 mM

Sample #2

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Early Endosome autoantigen 1	[U-15N]		1.0 mM

Sample #3

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Early Endosome autoantigen 1	[U-15N; U-13C]		1.0 mM

10 3D CBCA(CO)NH

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Early Endosome autoantigen 1			1.0 mM

Sample #2

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Early Endosome autoantigen 1	[U-15N]		1.0 mM

Sample #3

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Early Endosome autoantigen 1	[U-15N; U-13C]		1.0 mM

11 3D HNHA

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Early Endosome autoantigen 1			1.0 mM

Sample #2

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Early Endosome autoantigen 1	[U-15N]		1.0 mM

Sample #3

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Early Endosome autoantigen 1	[U-15N; U-13C]		1.0 mM

12 3D HNCO

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Early Endosome autoantigen 1			1.0 mM

Sample #2

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Early Endosome autoantigen 1	[U-15N]		1.0 mM

Sample #3

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Early Endosome autoantigen 1	[U-15N; U-13C]		1.0 mM

13 3D HCC TOCSY

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Early Endosome autoantigen 1			1.0 mM

Sample #2

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Early Endosome autoantigen 1	[U-15N]		1.0 mM

Sample #3

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Early Endosome autoantigen 1	[U-15N; U-13C]		1.0 mM

14 3D CCC TOCSY

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Early Endosome autoantigen 1			1.0 mM

Sample #2

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Early Endosome autoantigen 1	[U-15N]		1.0 mM

Sample #3

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Early Endosome autoantigen 1	[U-15N; U-13C]		1.0 mM

15 3D 1H-15N NOESY

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Early Endosome autoantigen 1			1.0 mM

Sample #2

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Early Endosome autoantigen 1	[U-15N]		1.0 mM

Sample #3

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Early Endosome autoantigen 1	[U-15N; U-13C]		1.0 mM

16 3D 1H-13C NOESY

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Early Endosome autoantigen 1			1.0 mM

Sample #2

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Early Endosome autoantigen 1	[U-15N]		1.0 mM

Sample #3

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Early Endosome autoantigen 1	[U-15N; U-13C]		1.0 mM

nullKeywords resonance assignment, FYVE, EEA1

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Found 1 Document (2.022 seconds)

BMRB: 4579

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Chemical shift reference

Related entities 1. Early Endosome autoantigen 1, : 3 : 36 entities Detail

Interaction partners 1. Early Endosome autoantigen 1, : 48 interactors Detail

Experiments performed 16 experiments Detail

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