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Backbone 1H,13C,and 15N assignments of the anti-dansyl antibody Fv fragment
Authors
Shindo, K., Masuda, K., Takahashi, H., Arata, Y., Shimada, I.
Assembly
anti-dansyl Fv fragment
Entity
1. VH domain (polymer, Thiol state: all disulfide bound), 124 monomers, 14136.65 Da Detail

EVKLEESGGG LVQPGGSMKL SCATSGFTFS DAWMDWVRQS PEKGLEWVAE IRNKANNHAT YYAESVKGRF TISRDDSKRR VYLQMNTLRA EDTGIYYCTG IYYHYPWFAY WGQGTLVTVS AEPR


2. VL domain (polymer, Thiol state: all disulfide bound), 113 monomers, 12372.83 Da Detail

DVVMTQTPLS LPVSLGNQAS ISCRSSQSLV HSNGNTYLHW YLQKPGQSPK LLIYKVSNRF SGVPDRFSGS GSGTDFTLKI SRVEAEDLGV YFCSQSTHVP FTFGSGTKLE IKR


Total weight
26509.48 Da
Max. entity weight
14136.65 Da
Entity Connection
disulfide 2 Detail

IDTypeValue orderAtom ID 1Atom ID 2
1disulfidesing1:CYS22:SG1:CYS98:SG
2disulfidesing2:CYS23:SG2:CYS93:SG

Source organism
Mus musculus
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete1
Sequence coverage: 52.3 %, Completeness: 13.9 %, Completeness (bb): 26.1 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All13.9 % (384 of 2753) 9.5 % (134 of 1417)12.0 % (129 of 1072)45.8 % (121 of 264)
Backbone26.1 % (360 of 1380)24.4 % (113 of 464)18.4 % (127 of 689)52.9 % (120 of 227)
Sidechain 2.0 % (32 of 1588) 2.2 % (21 of 953) 1.7 % (10 of 598) 2.7 % (1 of 37)
Aromatic 0.7 % (2 of 296) 0.7 % (1 of 148) 0.0 % (0 of 142)16.7 % (1 of 6)
Methyl 2.6 % (6 of 228) 2.6 % (3 of 114) 2.6 % (3 of 114)

1. VH domain

EVKLEESGGG LVQPGGSMKL SCATSGFTFS DAWMDWVRQS PEKGLEWVAE IRNKANNHAT YYAESVKGRF TISRDDSKRR VYLQMNTLRA EDTGIYYCTG IYYHYPWFAY WGQGTLVTVS AEPR

2. VL domain

DVVMTQTPLS LPVSLGNQAS ISCRSSQSLV HSNGNTYLHW YLQKPGQSPK LLIYKVSNRF SGVPDRFSGS GSGTDFTLKI SRVEAEDLGV YFCSQSTHVP FTFGSGTKLE IKR

Sample

Temperature 310 (±1) K, pH 6.0 (±0.05)


#NameIsotope labelingTypeConcentration
1VH domain[U-95% 13C; U-95% 15N(except Cys and Trp); [98% 15N]-Cys; [98% 15N]-Trp]1.2 mM
2VL domain[U-95% 13C; U-95% 15N(except Cys and Trp); [98% 15N]-Cys; [98% 15N]-Trp]1.2 mM

Chem. Shift Complete2
Sequence coverage: 49.4 %, Completeness: 13.6 %, Completeness (bb): 25.2 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All13.6 % (748 of 5506) 9.3 % (263 of 2834)11.8 % (254 of 2144)43.8 % (231 of 528)
Backbone25.2 % (695 of 2760)23.5 % (218 of 928)17.9 % (247 of 1378)50.7 % (230 of 454)
Sidechain 2.1 % (66 of 3176) 2.4 % (45 of 1906) 1.7 % (20 of 1196) 1.4 % (1 of 74)
Aromatic 0.3 % (2 of 592) 0.3 % (1 of 296) 0.0 % (0 of 284) 8.3 % (1 of 12)
Methyl 3.1 % (14 of 456) 3.1 % (7 of 228) 3.1 % (7 of 228)

1. VH domain

EVKLEESGGG LVQPGGSMKL SCATSGFTFS DAWMDWVRQS PEKGLEWVAE IRNKANNHAT YYAESVKGRF TISRDDSKRR VYLQMNTLRA EDTGIYYCTG IYYHYPWFAY WGQGTLVTVS AEPR

2. VL domain

DVVMTQTPLS LPVSLGNQAS ISCRSSQSLV HSNGNTYLHW YLQKPGQSPK LLIYKVSNRF SGVPDRFSGS GSGTDFTLKI SRVEAEDLGV YFCSQSTHVP FTFGSGTKLE IKR

Sample

Temperature 310 (±1) K, pH 6.0 (±0.05)


#NameIsotope labelingTypeConcentration
1VH domain[U-95% 13C; U-95% 15N(except Cys and Trp); [98% 15N]-Cys; [98% 15N]-Trp]1.2 mM
2VL domain[U-95% 13C; U-95% 15N(except Cys and Trp); [98% 15N]-Cys; [98% 15N]-Trp]1.2 mM

Release date
2000-09-24
Citation
Letter to the Editor: Backbone 1H, 13C, and 15N resonance assignments of the anti-dansyl antibody Fv fragment
Shindo, K., Masuda, K., Takahashi, H., Arata, Y., Shimada, I.
J. Biomol. NMR (2000), 17, 357-358
Related entities 1. VH domain, : 1 : 1 : 1 : 147 entities Detail
Related entities 2. VL domain, : 1 : 2 : 240 entities Detail
Interaction partners 2. VL domain, : 4 interactors Detail
Experiments performed 5 experiments Detail
Keywords anti-dansyl antibody, heteronuclear NMR, resonace assignments