NMR STRUCTURAL DETERMINATION OF VISCOTOXIN A3 FROM VISCUM ALBUM L.
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | disulfide | sing | 1:CYS3:SG | 1:CYS40:SG |
2 | disulfide | sing | 1:CYS4:SG | 1:CYS32:SG |
3 | disulfide | sing | 1:CYS16:SG | 1:CYS26:SG |
Polymer type: polypeptide(L)
Total | 1H | |
---|---|---|
All | 94.6 % (246 of 260) | 94.6 % (246 of 260) |
Backbone | 95.6 % (87 of 91) | 95.6 % (87 of 91) |
Sidechain | 94.1 % (159 of 169) | 94.1 % (159 of 169) |
Aromatic | 50.0 % (4 of 8) | 50.0 % (4 of 8) |
Methyl | 100.0 % (18 of 18) | 100.0 % (18 of 18) |
1. Viscotoxin A3
KSCCPNTTGR NIYNACRLTG APRPTCAKLS GCKIISGSTC PSDYPKPressure 1 atm, Temperature 285 (±0.1) K, pH 3.6
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Viscotoxin A3 | 1.0 ~ 2.0 mM | ||
2 | H3PO4/NaOH aqueous buffer | 50 mM | ||
3 | H2O | 90 % | ||
4 | D2O | 10 % |