BMRB: 4592

Solution structure of the syndecan-4 whole cytoplasmic domain in the presence of phosphatidylinositol 4,5-bisphosphate

Authors

Shin, J., Oh, E.S., Lee, D., Couchman, J.R., Lee, W.

Assembly

Syndecan 4

Entity

1. Syndecan 4 (polymer, Thiol state: not present), 28 monomers, 3306.785 × 2 Da Detail

RMKKKDEGSY DLGKKPIYKK APTNEFYA

2. PT5 (non-polymer), 1047.088 Da

Total weight

7660.6577 Da

Max. entity weight

3306.785 Da

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.9 %, Completeness: 80.8 %, Completeness (bb): 91.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	80.8 % (156 of 193)	80.8 % (156 of 193)
Backbone	91.1 % (51 of 56)	91.1 % (51 of 56)
Sidechain	76.6 % (105 of 137)	76.6 % (105 of 137)
Aromatic	100.0 % (17 of 17)	100.0 % (17 of 17)
Methyl	100.0 % (7 of 7)	100.0 % (7 of 7)

1. Syndecan 4

RMKKKDEGSY DLGKKPIYKK APTNEFYA

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 7.4

#	Name	Isotope labeling	Type	Concentration
1	Syndecan 4			2.0 ~ 4.0 mM
2	PIP2			0.5 ~ 1.0 mM
3	phosphate buffer			50 mM
4	H2O			90 %
5	D2O			10 %

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Calculated from 15 models in PDB: 1EJQ, Strand ID: A, B Detail

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Release date

2001-03-05

Citation

Solution structure of a syndecan-4 cytoplasmic domain and its interaction with phosphatidylinositol 4,5-bisphosphate
Lee, D., Oh, E.S., Woods, A., Couchman, J.R., Lee, W.
J. Biol. Chem. (1998), 273, 13022-13029, PubMed 9582338 , DOI http://dx.doi.org/10.1074/jbc.273.21.13022 , Abstract

Related entities 1. Syndecan 4, : 1 : 10 : 17 entities Detail

Interaction partners 1. Syndecan 4, : 8 interactors Detail

Experiments performed 2 experiments Detail