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Solution structure of the syndecan-4 whole cytoplasmic domain in the presence of phosphatidylinositol 4,5-bisphosphate
Authors
Shin, J., Oh, E.S., Lee, D., Couchman, J.R., Lee, W.
Assembly
Syndecan 4
Entity
1. Syndecan 4 (polymer, Thiol state: not present), 28 monomers, 3306.785 × 2 Da Detail

RMKKKDEGSY DLGKKPIYKK APTNEFYA


2. PT5 (non-polymer), 1047.088 Da
Total weight
7660.6577 Da
Max. entity weight
3306.785 Da
Exptl. method
NMR
Refine. method
simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 92.9 %, Completeness: 82.9 %, Completeness (bb): 90.7 % Detail

Polymer type: polypeptide(L)

Total1H
All82.9 % (160 of 193)82.9 % (160 of 193)
Backbone90.7 % (49 of 54)90.7 % (49 of 54)
Sidechain79.9 % (111 of 139)79.9 % (111 of 139)
Aromatic100.0 % (17 of 17)100.0 % (17 of 17)
Methyl100.0 % (7 of 7)100.0 % (7 of 7)

1. Syndecan 4

RMKKKDEGSY DLGKKPIYKK APTNEFYA

Sample

Pressure 1 atm, Temperature 298 K, pH 7.4


#NameIsotope labelingTypeConcentration
1Syndecan 42.0 ~ 4.0 mM
2PIP20.5 ~ 1.0 mM
3phosphate buffer50 mM
4H2O90 %
5D2O10 %

Protein Blocks Logo
Calculated from 15 models in PDB: 1EJQ, Strand ID: A, B Detail


Release date
2001-03-05
Citation
Solution structure of a syndecan-4 cytoplasmic domain and its interaction with phosphatidylinositol 4,5-bisphosphate
Lee, D., Oh, E.S., Woods, A., Couchman, J.R., Lee, W.
J. Biol. Chem. (1998), 273, 13022-13029, PubMed 9582338 , DOI http://dx.doi.org/10.1074/jbc.273.21.13022 ,
Related entities 1. Syndecan 4, : 1 : 7 : 10 entities Detail
Interaction partners 1. Syndecan 4, : 8 interactors Detail
Keywords symmetric dimer, complexed with PIP2