BMRB: 4620

Human prion protein variant R220K

Authors

Calzonai, L., Lysek, D.A., Guntert, P., Von Schroetter, C., Zahn, R., Riek, R., Wuthrich, K.

Assembly

prion protein

Entity

1. prion protein (polymer, Thiol state: all disulfide bound), 112 monomers, 13117.45 Da Detail

GSVVGGLGGY MLGSAMSRPI IHFGSDYEDR YYRENMHRYP NQVYYRPMDE YSNQNNFVHD CVNITIKQHT VTTTTKGENF TETDVKMMER VVEQMCITQY EKESQAYYQR GS

Formula weight

13117.45 Da

Entity Connection

disulfide 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS61:SG	1:CYS96:SG

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 87.6 %, Completeness (bb): 82.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	87.6 % (1148 of 1310)	97.5 % (666 of 683)	72.0 % (363 of 504)	96.7 % (119 of 123)
Backbone	82.3 % (548 of 666)	97.4 % (224 of 230)	67.0 % (219 of 327)	96.3 % (105 of 109)
Sidechain	93.8 % (701 of 747)	97.6 % (442 of 453)	87.5 % (245 of 280)	100.0 % (14 of 14)
Aromatic	73.1 % (98 of 134)	94.0 % (63 of 67)	52.2 % (35 of 67)
Methyl	100.0 % (86 of 86)	100.0 % (43 of 43)	100.0 % (43 of 43)

1. prion protein

GSVVGGLGGY MLGSAMSRPI IHFGSDYEDR YYRENMHRYP NQVYYRPMDE YSNQNNFVHD CVNITIKQHT VTTTTKGENF TETDVKMMER VVEQMCITQY EKESQAYYQR GS

Show sample condition

Sample

Temperature 293 K, pH 4.5

#	Name	Isotope labeling	Type	Concentration
1	prion protein	[U-13C; U-15N]		1.0 mM
2	sodium acetate			50 mM

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LACS Plot; CA

Referencing offset: 0.31 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 ppm	null	null	0.0
1H	H2O	protons	4.78 ppm	internal	direct	0.0
15N	na	na	0.0 ppm	null	null	0.0

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LACS Plot; CB

Referencing offset: 0.31 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 ppm	null	null	0.0
1H	H2O	protons	4.78 ppm	internal	direct	0.0
15N	na	na	0.0 ppm	null	null	0.0

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LACS Plot; HA

Referencing offset: 0.06 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 ppm	null	null	0.0
1H	H2O	protons	4.78 ppm	internal	direct	0.0
15N	na	na	0.0 ppm	null	null	0.0

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Protein Blocks Logo

Calculated from 20 models in PDB: 1E1U, Strand ID: A Detail

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Release date

2000-09-06

Citation

NMR structures of three single-residue variants of the human prion protein
Calzonai, L., Lysek, D.A., Guntert, P., Von Schroetter, C., Zahn, R., Riek, R., Wuthrich, K.
Proc. Natl. Acad. Sci. U. S. A. (2000), 97, 8340-8345, PubMed 10900000 , DOI: , Abstract

Related entities 1. prion protein, : 1 : 30 : 1 : 130 entities Detail

Interaction partners 1. prion protein, : 70 interactors Detail

Experiments performed 5 experiments Detail