BMRB: 4621

N-terminal RING finger domain of human NOT-4

Authors

Hanzawa, H., de Ruwe, M.J., Albert, T.K., van der Vliet, P.C., Timmers, H.T., Boelens, R.

Assembly

NOT4

Entity

1. NOT4 (polymer, Thiol state: not reported), 89 monomers, 10285.75 Da Detail

GLVPRGSHML EMSRSPDAKE DPVECPLCME PLEIDDINFF PCTCGYQICR FCWHRIRTDE NGLCPACRKP YPEDPAVYKP LSQQELQRI

2. ZN (non-polymer), 65.409 × 2 Da

Total weight

10416.568 Da

Max. entity weight

10285.75 Da

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.9 %, Completeness: 82.3 %, Completeness (bb): 93.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	82.3 % (862 of 1047)	80.0 % (445 of 556)	82.8 % (336 of 406)	95.3 % (81 of 85)
Backbone	93.0 % (476 of 512)	92.4 % (158 of 171)	91.6 % (241 of 263)	98.7 % (77 of 78)
Sidechain	75.8 % (470 of 620)	74.5 % (287 of 385)	78.5 % (179 of 228)	57.1 % (4 of 7)
Aromatic	37.8 % (28 of 74)	62.2 % (23 of 37)	11.1 % (4 of 36)	100.0 % (1 of 1)
Methyl	85.7 % (60 of 70)	85.7 % (30 of 35)	85.7 % (30 of 35)

1. NOT4

GLVPRGSHML EMSRSPDAKE DPVECPLCME PLEIDDINFF PCTCGYQICR FCWHRIRTDE NGLCPACRKP YPEDPAVYKP LSQQELQRI

Show sample condition

Sample

Temperature 300 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	NOT4			0.0 ~ 0.0 mM

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LACS Plot; CA

Referencing offset: 2.16 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 ppm	null	null	0.0
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0

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LACS Plot; CB

Referencing offset: 2.16 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 ppm	null	null	0.0
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0

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LACS Plot; HA

Referencing offset: 0.05 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 ppm	null	null	0.0
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0

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LACS Plot; CO

Referencing offset: 2.48 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 ppm	null	null	0.0
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0

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Protein Blocks Logo

Calculated from 30 models in PDB: 1E4U, Strand ID: A Detail

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Release date

2001-05-01

Citation

The structure of the C4C4 ring finger of human NOT4 reveals features distinct from those of C3HC4 RING fingers
Hanzawa, H., de Ruwe, M.J., Albert, T.K., van der Vliet, P.C., Timmers, H.T., Boelens, R.
J. Biol. Chem. (2001), 276, 10185-10190, PubMed 11087754 , DOI 10.1074/jbc.M009298200 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. NOT4, : 1 : 21 entities Detail

Interaction partners 1. NOT4, : 2 interactors Detail

Experiments performed 1 experiments Detail