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Found 1 Document (1.576 seconds)

BMRB: 4633

1FUW

Solution Structure, Backbone Dynamics, and stability of a Double Mutant Single-Chain Monellin. Structural origin of sweetness

Authors

Sung, Y.H., Shin, J., Chang, H.J., Cho, J.M., Lee, W.

Assembly

MONELLIN

Entity

1. MONELLIN (polymer, Thiol state: all free), 91 monomers, 10824.23 Da Detail

GEWEIIEIGP FYQNLGKFAV DEENKIGQYG RLTFNKVIKP CMKKTIYENE REIKGYEYQL YVYASDKLFR ADISEDYKTR GRKLLRFNGP V

Formula weight

10824.23 Da

Source organism

Dioscoreophyllum cumminsii

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.8 %, Completeness: 86.8 %, Completeness (bb): 94.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H
All	86.8 % (526 of 606)	86.8 % (526 of 606)
Backbone	94.1 % (176 of 187)	94.1 % (176 of 187)
Sidechain	83.5 % (350 of 419)	83.5 % (350 of 419)
Aromatic	71.4 % (45 of 63)	71.4 % (45 of 63)
Methyl	88.1 % (37 of 42)	88.1 % (37 of 42)

1. MONELLIN

GEWEIIEIGP FYQNLGKFAV DEENKIGQYG RLTFNKVIKP CMKKTIYENE REIKGYEYQL YVYASDKLFR ADISEDYKTR GRKLLRFNGP V

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Concentration
1	MONELLIN	2 mM
2	photassium phosphate	50 mM
3	H20	90 %
4	D20	10 %

Protein Blocks Logo

Calculated from 21 models in PDB: 1FUW, Strand ID: A Detail

Release date

2002-12-22

Citation

Solution structure, backbone dynamics, and stability of a double mutant single-chain monellin. structural origin of sweetness
Sung, Y.H., Shin, J., Chang, H.J., Cho, J.M., Lee, W.
J. Biol. Chem. (2001), 276, 19624-19630, PubMed 11279156 , DOI 10.1074/jbc.M100930200 , Abstract

Related entities 1. MONELLIN, : 1 : 45 entities Detail

Experiments performed 5 experiments Detail

1 2D NOESY

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Concentration
1	MONELLIN	2 mM
2	photassium phosphate	50 mM
3	H20	90 %
4	D20	10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
5	MONELLIN	[U-15N]	2 mM
6	photassium phosphate		50 mM
7	H20		90 %
8	D20		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Concentration
9	MONELLIN	2 mM
10	photassium phosphate	50 mM
11	D20	100 %

Sample #4

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
12	MONELLIN	[U-15N]	2 mM
13	photassium phosphate		50 mM
14	D20		100 %

2 3D 15N-separated NOESY

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Concentration
1	MONELLIN	2 mM
2	photassium phosphate	50 mM
3	H20	90 %
4	D20	10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
5	MONELLIN	[U-15N]	2 mM
6	photassium phosphate		50 mM
7	H20		90 %
8	D20		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Concentration
9	MONELLIN	2 mM
10	photassium phosphate	50 mM
11	D20	100 %

Sample #4

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
12	MONELLIN	[U-15N]	2 mM
13	photassium phosphate		50 mM
14	D20		100 %

3 DQF-COSY

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Concentration
1	MONELLIN	2 mM
2	photassium phosphate	50 mM
3	H20	90 %
4	D20	10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
5	MONELLIN	[U-15N]	2 mM
6	photassium phosphate		50 mM
7	H20		90 %
8	D20		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Concentration
9	MONELLIN	2 mM
10	photassium phosphate	50 mM
11	D20	100 %

Sample #4

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
12	MONELLIN	[U-15N]	2 mM
13	photassium phosphate		50 mM
14	D20		100 %

4 HNHA

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Concentration
1	MONELLIN	2 mM
2	photassium phosphate	50 mM
3	H20	90 %
4	D20	10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
5	MONELLIN	[U-15N]	2 mM
6	photassium phosphate		50 mM
7	H20		90 %
8	D20		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Concentration
9	MONELLIN	2 mM
10	photassium phosphate	50 mM
11	D20	100 %

Sample #4

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
12	MONELLIN	[U-15N]	2 mM
13	photassium phosphate		50 mM
14	D20		100 %

5 HSQC(exchange)

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Concentration
1	MONELLIN	2 mM
2	photassium phosphate	50 mM
3	H20	90 %
4	D20	10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
5	MONELLIN	[U-15N]	2 mM
6	photassium phosphate		50 mM
7	H20		90 %
8	D20		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Concentration
9	MONELLIN	2 mM
10	photassium phosphate	50 mM
11	D20	100 %

Sample #4

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
12	MONELLIN	[U-15N]	2 mM
13	photassium phosphate		50 mM
14	D20		100 %

nullKeywords beta-sheet, alpha-helix, loop

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Found 1 Document (1.576 seconds)

BMRB: 4633

Sample

Related entities 1. MONELLIN, : 1 : 45 entities Detail

Experiments performed 5 experiments Detail

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