BMRBj - BMREntryMaster

	Total	¹H	¹⁵N
All	85.2 % (623 of 731)	83.5 % (528 of 632)	96.0 % (95 of 99)
Backbone	97.5 % (278 of 285)	96.4 % (188 of 195)	100.0 % (90 of 90)
Sidechain	77.4 % (345 of 446)	77.8 % (340 of 437)	55.6 % (5 of 9)
Aromatic	89.2 % (58 of 65)	89.1 % (57 of 64)	100.0 % (1 of 1)
Methyl	95.3 % (41 of 43)	95.3 % (41 of 43)

1. MNEI SWEET PROTEIN RELATED TO MONELLIN

GEWEIIDIGP FTQNLGKFAV DEENKIGQYG RLTFNKVIRP CMKKTIYENE GFREIKGYEY QLYVYASDKL FRADISEDYK TRGRKLLRFN GPVPPP

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Sample

Pressure 1 atm, Temperature 308 K, pH 2.9

#	Name	Isotope labeling	Concentration
1	MNEI SWEET PROTEIN RELATED TO MONELLIN	[U-15N]	2 mM
2	phosphate buffer K		18.5 mM
3	H2O		90 %
4	D2O		10 %

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Calculated from 20 models in PDB: 1FA3, Strand ID: A Detail

Coupling constant

114 J values in 3 lists
Field strength (¹H) 600 MHz, Pressure 1 atm, Temperature 308 K, pH 2.9 Detail

Release date

2001-05-06

Citation

Solution structure of a sweet protein: NMR study of MNEI, a single chain monellin
Spadaccini, R., Crescenzi, O.O., tancredi, T., De casamassimi, N., saviano, G., scognamiglio, R., Di Donato, A., Temussi, P.A.
J. Mol. Biol. (2001), 305, 505-514, PubMed 11152608 , DOI 10.1006/jmbi.2000.4304 , Abstract

Related entities 1. MNEI, A SINGLE CHAIN SWEET PROTEIN, : 1 : 3 : 13 : 30 entities Detail

Experiments performed 6 experiments Detail

1 3D 15N-separated NOESY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 308 K, pH 2.9

#	Name	Isotope labeling	Concentration
1	MNEI SWEET PROTEIN RELATED TO MONELLIN	[U-15N]	2 mM
2	phosphate buffer K		18.5 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 308 K, pH 2.9

#	Name	Concentration
5	MNEI SWEET PROTEIN RELATED TO MONELLIN	2 mM
6	phosphate buffer K	18.5 mM
7	H2O	90 %
8	D2O	10 %

Sample #3

Pressure 1 atm, Temperature 308 K, pH 2.9

#	Name	Concentration
9	MNEI SWEET PROTEIN RELATED TO MONELLIN	2 mM
10	phosphate buffer K	18.5 mM
11	D2O	100 %

2 HNHA

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 308 K, pH 2.9

#	Name	Isotope labeling	Concentration
1	MNEI SWEET PROTEIN RELATED TO MONELLIN	[U-15N]	2 mM
2	phosphate buffer K		18.5 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 308 K, pH 2.9

#	Name	Concentration
5	MNEI SWEET PROTEIN RELATED TO MONELLIN	2 mM
6	phosphate buffer K	18.5 mM
7	H2O	90 %
8	D2O	10 %

Sample #3

Pressure 1 atm, Temperature 308 K, pH 2.9

#	Name	Concentration
9	MNEI SWEET PROTEIN RELATED TO MONELLIN	2 mM
10	phosphate buffer K	18.5 mM
11	D2O	100 %

3 HSQC

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 308 K, pH 2.9

#	Name	Isotope labeling	Concentration
1	MNEI SWEET PROTEIN RELATED TO MONELLIN	[U-15N]	2 mM
2	phosphate buffer K		18.5 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 308 K, pH 2.9

#	Name	Concentration
5	MNEI SWEET PROTEIN RELATED TO MONELLIN	2 mM
6	phosphate buffer K	18.5 mM
7	H2O	90 %
8	D2O	10 %

Sample #3

Pressure 1 atm, Temperature 308 K, pH 2.9

#	Name	Concentration
9	MNEI SWEET PROTEIN RELATED TO MONELLIN	2 mM
10	phosphate buffer K	18.5 mM
11	D2O	100 %

4 HNHB

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 308 K, pH 2.9

#	Name	Isotope labeling	Concentration
1	MNEI SWEET PROTEIN RELATED TO MONELLIN	[U-15N]	2 mM
2	phosphate buffer K		18.5 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 308 K, pH 2.9

#	Name	Concentration
5	MNEI SWEET PROTEIN RELATED TO MONELLIN	2 mM
6	phosphate buffer K	18.5 mM
7	H2O	90 %
8	D2O	10 %

Sample #3

Pressure 1 atm, Temperature 308 K, pH 2.9

#	Name	Concentration
9	MNEI SWEET PROTEIN RELATED TO MONELLIN	2 mM
10	phosphate buffer K	18.5 mM
11	D2O	100 %

5 E-COSY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 308 K, pH 2.9

#	Name	Isotope labeling	Concentration
1	MNEI SWEET PROTEIN RELATED TO MONELLIN	[U-15N]	2 mM
2	phosphate buffer K		18.5 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 308 K, pH 2.9

#	Name	Concentration
5	MNEI SWEET PROTEIN RELATED TO MONELLIN	2 mM
6	phosphate buffer K	18.5 mM
7	H2O	90 %
8	D2O	10 %

Sample #3

Pressure 1 atm, Temperature 308 K, pH 2.9

#	Name	Concentration
9	MNEI SWEET PROTEIN RELATED TO MONELLIN	2 mM
10	phosphate buffer K	18.5 mM
11	D2O	100 %

6 2D NOESY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 308 K, pH 2.9

#	Name	Isotope labeling	Concentration
1	MNEI SWEET PROTEIN RELATED TO MONELLIN	[U-15N]	2 mM
2	phosphate buffer K		18.5 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 308 K, pH 2.9

#	Name	Concentration
5	MNEI SWEET PROTEIN RELATED TO MONELLIN	2 mM
6	phosphate buffer K	18.5 mM
7	H2O	90 %
8	D2O	10 %

Sample #3

Pressure 1 atm, Temperature 308 K, pH 2.9

#	Name	Concentration
9	MNEI SWEET PROTEIN RELATED TO MONELLIN	2 mM
10	phosphate buffer K	18.5 mM
11	D2O	100 %

nullKeywords 1 helix, 5 stranded beta sheet

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BMRB: 4638

Sample

Related entities 1. MNEI, A SINGLE CHAIN SWEET PROTEIN, : 1 : 3 : 13 : 30 entities Detail

Experiments performed 6 experiments Detail

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