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SOLUTION STRUCTURE OF MNEI, A SWEET PROTEIN
Authors
Temussi, P.A., Spadaccini, R.
Assembly
MNEI, A SINGLE CHAIN SWEET PROTEIN
Entity
1. MNEI, A SINGLE CHAIN SWEET PROTEIN (polymer, Thiol state: all free), 96 monomers, 11271.72 Da Detail

GEWEIIDIGP FTQNLGKFAV DEENKIGQYG RLTFNKVIRP CMKKTIYENE GFREIKGYEY QLYVYASDKL FRADISEDYK TRGRKLLRFN GPVPPP


Formula weight
11271.72 Da
Source organism
Dioscoreophyllum cumminsii
Exptl. method
NMR
Refine. method
torsion angle dynamics
Data set
assigned_chemical_shifts, coupling_constants
Chem. Shift Complete
Sequence coverage: 97.9 %, Completeness: 84.3 %, Completeness (bb): 97.8 % Detail

Polymer type: polypeptide(L)

Total1H15N
All84.3 % (622 of 738)82.3 % (520 of 632)96.2 % (102 of 106)
Backbone97.8 % (270 of 276)96.8 % (180 of 186)100.0 % (90 of 90)
Sidechain76.2 % (352 of 462)76.2 % (340 of 446)75.0 % (12 of 16)
Aromatic89.2 % (58 of 65)89.1 % (57 of 64)100.0 % (1 of 1)
Methyl95.3 % (41 of 43)95.3 % (41 of 43)

1. MNEI SWEET PROTEIN RELATED TO MONELLIN

GEWEIIDIGP FTQNLGKFAV DEENKIGQYG RLTFNKVIRP CMKKTIYENE GFREIKGYEY QLYVYASDKL FRADISEDYK TRGRKLLRFN GPVPPP

Sample

Pressure 1 atm, Temperature 308 K, pH 2.9


#NameIsotope labelingTypeConcentration
1MNEI SWEET PROTEIN RELATED TO MONELLIN[U-15N]2 mM
2phosphate buffer K18.5 mM
3H2O90 %
4D2O10 %

Protein Blocks Logo
Calculated from 20 models in PDB: 1FA3, Strand ID: A Detail


Coupling constant
114 J values in 3 lists
Field strength (1H) 600 MHz, Pressure 1 atm, Temperature 308 K, pH 2.9 Detail
Release date
2001-05-06
Citation
Solution structure of a sweet protein: NMR study of MNEI, a single chain monellin
Spadaccini, R., Crescenzi, O.O., tancredi, T., De casamassimi, N., saviano, G., scognamiglio, R., Di Donato, A., Temussi, P.A.
J. Mol. Biol. (2001), 305, 505-514, PubMed 11152608 , DOI 10.1006/jmbi.2000.4304 ,
Related entities 1. MNEI, A SINGLE CHAIN SWEET PROTEIN, : 1 : 2 : 12 : 17 entities Detail
Keywords 1 helix, 5 stranded beta sheet