1H, 13C and 15N resonance assignments of the DNA binding domain of the human forkhead transcription factor AFX
GSSHHHHHHS SGLVPRGSHM LEDPGAVTGP RKGGSRRNAW GNQSYAELIS QAIESAPEKR LTLAQIYEWM VRTVPYFKDK GDSNSSAGWK NSIRHNLSLH SKFIKVHNEA TGKSSWWMLN PEGGKSGKAP RRRAASMDSS SKLLRGRSKA
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 73.2 % (1252 of 1711) | 66.2 % (596 of 900) | 79.2 % (517 of 653) | 88.0 % (139 of 158) |
Backbone | 84.7 % (750 of 886) | 82.5 % (254 of 308) | 85.5 % (372 of 435) | 86.7 % (124 of 143) |
Sidechain | 64.8 % (622 of 960) | 57.8 % (342 of 592) | 75.1 % (265 of 353) | 100.0 % (15 of 15) |
Aromatic | 53.5 % (77 of 144) | 55.6 % (40 of 72) | 47.8 % (32 of 67) | 100.0 % (5 of 5) |
Methyl | 60.7 % (68 of 112) | 25.0 % (14 of 56) | 96.4 % (54 of 56) |
1. AFX
GSSHHHHHHS SGLVPRGSHM LEDPGAVTGP RKGGSRRNAW GNQSYAELIS QAIESAPEKR LTLAQIYEWM VRTVPYFKDK GDSNSSAGWK NSIRHNLSLH SKFIKVHNEA TGKSSWWMLN PEGGKSGKAP RRRAASMDSS SKLLRGRSKATemperature 304 (±0.5) K, pH 6.3 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | AFX | [U-13C; U-15N] | 1.5 mM | |
2 | Na phosphate | 20 mM | ||
3 | NaCl | 100 mM | ||
4 | EDTA | 1 mM | ||
5 | Pefabloc | 1 mM | ||
6 | NaN3 | 0.02 % w/v |