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Assignment of 1H, 13C and 15N Resonances in Unfolded Apomyoglobin at pH 2.3
Authors
Yao, J., Chung, J., Eliezer, D., Wright, P.E., Dyson, J.
Assembly
apomyoglobin pH 2.3
Entity
1. apomyoglobin pH 2.3 (polymer, Thiol state: not present), 153 monomers, 17199.65 Da Detail

VLSEGEWQLV LHVWAKVEAD VAGHGQDILI RLFKSHPETL EKFDRFKHLK TEAEMKASED LKKHGVTVLT ALGAILKKKG HHEAELKPLA QSHATKHKIP IKYLEFISEA IIHVLHSRHP GDFGADAQGA MNKALELFRK DIAAKYKELG YQG


Formula weight
17199.65 Da
Source organism
Physeter catodon
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 99.3 %, Completeness: 50.4 %, Completeness (bb): 96.8 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All50.4 % (925 of 1836)33.6 % (320 of 953)63.4 % (458 of 722)91.3 % (147 of 161)
Backbone96.8 % (870 of 899)92.7 % (280 of 302)98.9 % (443 of 448)98.7 % (147 of 149)
Sidechain18.2 % (196 of 1079) 6.1 % (40 of 651)37.5 % (156 of 416) 0.0 % (0 of 12)
Aromatic 0.0 % (0 of 156) 0.0 % (0 of 78) 0.0 % (0 of 76) 0.0 % (0 of 2)
Methyl17.9 % (33 of 184) 8.7 % (8 of 92)27.2 % (25 of 92)

1. unfolded myoglobin

VLSEGEWQLV LHVWAKVEAD VAGHGQDILI RLFKSHPETL EKFDRFKHLK TEAEMKASED LKKHGVTVLT ALGAILKKKG HHEAELKPLA QSHATKHKIP IKYLEFISEA IIHVLHSRHP GDFGADAQGA MNKALELFRK DIAAKYKELG YQG

Sample

Temperature 298 (±1) K, pH 2.3 (±0.1)


#NameIsotope labelingTypeConcentration
1unfolded myoglobin[U-95% 13C; U-95% 15N]0.2 mM

LACS Plot; CA
Referencing offset: -0.07 ppm, Outliers: 5 Detail
LACS Plot; CB
Referencing offset: -0.07 ppm, Outliers: 5 Detail
LACS Plot; HA
Referencing offset: 0.03 ppm, Outliers: 2 Detail
LACS Plot; CO
Referencing offset: 0.21 ppm, Outliers: 2 Detail
Release date
2001-08-08
Citation
NMR structural and dynamic characterization of the acid-unfolded state of apomyoglobin provides insights into the early events in protein folding
Yao, J., Chung, J., Eliezer, D., Wright, P.E., Dyson, H.JANE.
Biochemistry (2001), 40, 3561-3571, PubMed 11297422 , DOI 10.1021/bi002776i ,
Related entities 1. apomyoglobin pH 2.3, : 1 : 93 : 7 : 101 entities Detail
Interaction partners 1. apomyoglobin pH 2.3, : 2 interactors Detail
Experiments performed 2 experiments Detail
Keywords myoglobin, unfolded