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1H, 15N, 13C, and 13CO Assignments for ZipA
Authors
Moy, F.J., Glasfeld, E., Powers, R.
Assembly
C-terminal FtsZ binding domain of E. coli ZipA
Entity
1. C-terminal FtsZ binding domain of E. coli ZipA (polymer, Thiol state: not present), 144 monomers, 16116.26 Da Detail

MDKPKRKEAV IIMNVAAHHG SELNGELLLN SIQQAGFIFG DMNIYHRHLS PDGSGPALFS LANMVKPGTF DPEMKDFTTP GVTIFMQVPS YGDELQNFKL MLQSAQHIAD EVGGVVLDDQ RRMMTPQKLR EYQDIIREVK DANA


Formula weight
16116.26 Da
Source organism
Escherichia coli
Exptl. method
NMR
Refine. method
distance geometry simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 90.6 %, Completeness (bb): 98.1 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All90.6 % (1530 of 1688)88.9 % (781 of 879)93.9 % (611 of 651)87.3 % (138 of 158)
Backbone98.1 % (821 of 837)98.9 % (277 of 280)97.4 % (410 of 421)98.5 % (134 of 136)
Sidechain85.6 % (842 of 984)84.1 % (504 of 599)92.0 % (334 of 363)18.2 % (4 of 22)
Aromatic58.8 % (67 of 114)59.6 % (34 of 57)57.9 % (33 of 57)
Methyl98.7 % (148 of 150)98.7 % (74 of 75)98.7 % (74 of 75)

1. E. coli ZipA

MDKPKRKEAV IIMNVAAHHG SELNGELLLN SIQQAGFIFG DMNIYHRHLS PDGSGPALFS LANMVKPGTF DPEMKDFTTP GVTIFMQVPS YGDELQNFKL MLQSAQHIAD EVGGVVLDDQ RRMMTPQKLR EYQDIIREVK DANA

Sample #1

Temperature 298 (±0.2) K, pH 6.0 (±0.05)


#NameIsotope labelingTypeConcentration
1E. coli ZipA[U-15N]1.0 mM
2potassium phosphate50 mM
3NaN32 mM
4DTT[U-2H]50 mM
5H2O90 %
6D2O10 %
Sample #2

Temperature 298 (±0.2) K, pH 6.0 (±0.05)


#NameIsotope labelingTypeConcentration
7E. coli ZipA[U-13C; U-15N]1.0 mM
8potassium phosphate50 mM
9NaN32 mM
10DTT[U-2H]50 mM
11H2O90 %
12D2O10 %

LACS Plot; CA
Referencing offset: 0.13 ppm, Outliers: 2 Detail
LACS Plot; CB
Referencing offset: 0.13 ppm, Outliers: 2 Detail
LACS Plot; HA
Referencing offset: -0.06 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 0.03 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 1 models in PDB: 1F7W, Strand ID: A Detail


Release date
2000-05-23
Citation
Letter to the Editor: 1H, 15N, 13C, and 13CO assignments and secondary structure determination of ZipA*
Moy, F.J., Glasfeld, E., Powers, R.
J. Biomol. NMR (2000), 17, 275-276, PubMed , DOI:
Related entities 1. C-terminal FtsZ binding domain of E. coli ZipA, : 1 : 13 : 7 : 85 entities Detail
Interaction partners 1. C-terminal FtsZ binding domain of E. coli ZipA, : 2 interactors Detail
Keywords Bacterial Cell Division, ZipA, Resonance assignments, Secondary Structure