BMRBj - BMREntryMaster

Found 1 Document (1.705 seconds)

BMRB: 4726

1PQV

Structure of a Conserved Domain Common to the Transcription Factors TFIIS, elongin A, and CRSP70

Authors

Booth, V., Koth, C., Edwards, A., Arrowsmith, C.

Assembly

TFIIS domain I

Entity

1. TFIIS domain I (polymer, Thiol state: not present), 114 monomers, 12821.40 Da Detail

GSHMDSKEVL VHVKNLEKNK SNDAAVLEIL HVLDKEFVPT EKLLRETKVG VEVNKFKKST NVEISKLVKK MISSWKDAIN KNKRSRQAQQ HHQDHAPGNA EDKTTVGESV NGVQ

Formula weight

12821.4 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

NMR

Refine. method

simulated annealing molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 70.2 %, Completeness: 56.8 %, Completeness (bb): 57.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	56.8 % (767 of 1350)	61.7 % (438 of 710)	48.7 % (250 of 513)	62.2 % (79 of 127)
Backbone	57.5 % (391 of 680)	68.4 % (158 of 231)	46.6 % (157 of 337)	67.9 % (76 of 112)
Sidechain	57.8 % (450 of 779)	58.5 % (280 of 479)	58.6 % (167 of 285)	20.0 % (3 of 15)
Aromatic	32.1 % (18 of 56)	64.3 % (18 of 28)	0.0 % (0 of 27)	0.0 % (0 of 1)
Methyl	68.3 % (86 of 126)	73.0 % (46 of 63)	63.5 % (40 of 63)

1. TFIIS domain I

GSHMDSKEVL VHVKNLEKNK SNDAAVLEIL HVLDKEFVPT EKLLRETKVG VEVNKFKKST NVEISKLVKK MISSWKDAIN KNKRSRQAQQ HHQDHAPGNA EDKTTVGESV NGVQ

Show sample condition

Sample

Temperature 298 (±0.2) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	TFIIS domain I			0.0 ~ 0.0 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹³C	na	na	null	null
¹H	na	na	null	null
¹⁵N	na	na	null	null

Referencing offset: -0.21 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹³C	na	na	null	null
¹H	na	na	null	null
¹⁵N	na	na	null	null

Referencing offset: -0.21 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹³C	na	na	null	null
¹H	na	na	null	null
¹⁵N	na	na	null	null

Referencing offset: 0.05 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 1EO0, Strand ID: A Detail

Release date

2000-12-06

Citation

Structure of a conserved domain common to the transcription factors TFIIS, elongin A, and CRSP70
Booth, V., Koth, C.M., Edwards, A.M., Arrowsmith, C.H.
J. Biol. Chem. (2000), 275, 31266-31268, PubMed 10811649 , DOI 10.1074/jbc.M002595200 , Abstract

Related entities 1. TFIIS domain I, : 1 : 2 : 3 entities Detail

Interaction partners 1. TFIIS domain I, : 30 interactors Detail

More details for 30 interactors identified by 7 citations

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr4726_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr4726_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	cs_1	Warning	839		67.5 (chain: 1, length: 114)

Assigned chemical shifts

Saveframe: cs_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 114, Sequence coverage: 67.5%
- Total number of assignments
  - ¹⁵N chemical shifts: 79
  - ¹H chemical shifts: 515
  - ¹³C chemical shifts: 245
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹⁵N chemical shifts	18	3	16.7
¹H chemical shifts	479	270	56.4
¹³C chemical shifts	285	168	58.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	65	45	69.2
¹³C chemical shifts	65	40	61.5

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Found 1 Document (1.705 seconds)

BMRB: 4726

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. TFIIS domain I, : 1 : 2 : 3 entities Detail

Interaction partners 1. TFIIS domain I, : 30 interactors Detail

Chemical shift validation 3 contents Detail

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹⁵N chemical shifts	130	79	60.8
¹H chemical shifts	710	424	59.7
¹³C chemical shifts	513	245	47.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹⁵N chemical shifts	112	76	67.9
¹H chemical shifts	231	154	66.7
¹³C chemical shifts	228	77	33.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹⁵N chemical shifts	1	0	0.0
¹H chemical shifts	28	18	64.3
¹³C chemical shifts	27	0	0.0