BMRBj - BMREntryMaster

Found 1 Document (1.267 seconds)

BMRB: 4727

1EMW

Another piece of the Ribosome: Solution Structure of S16 and its Location in the 30S Subunit

Authors

Allard, P., Rak, A.V., Wimberly, B.T., Clemons, W.M., Kalinin, A., Helgstrand, M., Garber, M.B., Ramakrishnan, V., Hard, T.

Assembly

S16

Entity

1. S16 (polymer, Thiol state: not present), 88 monomers, 10386.80 Da Detail

MVKIRLARFG SKHNPHYRIV VTDARRKRDG KYIEKIGYYD PRKTTPDWLK VDVERARYWL SVGAQPTDTA RRLLRQAGVF RQEAREGA

Formula weight

10386.8 Da

Source organism

Thermus thermophilus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 85.0 %, Completeness (bb): 82.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	85.0 % (910 of 1071)	94.5 % (536 of 567)	70.3 % (291 of 414)	92.2 % (83 of 90)
Backbone	82.5 % (429 of 520)	96.1 % (171 of 178)	70.2 % (181 of 258)	91.7 % (77 of 84)
Sidechain	88.5 % (560 of 633)	93.8 % (365 of 389)	79.4 % (189 of 238)	100.0 % (6 of 6)
Aromatic	54.3 % (50 of 92)	100.0 % (46 of 46)	4.5 % (2 of 44)	100.0 % (2 of 2)
Methyl	100.0 % (90 of 90)	100.0 % (45 of 45)	100.0 % (45 of 45)

1. S16

MVKIRLARFG SKHNPHYRIV VTDARRKRDG KYIEKIGYYD PRKTTPDWLK VDVERARYWL SVGAQPTDTA RRLLRQAGVF RQEAREGA

Show sample condition

Sample #1

Temperature 303 (±1) K

#	Name	Isotope labeling	Type	Concentration
1	S16			1.5 mM

Sample #2

Temperature 303 (±1) K

#	Name	Isotope labeling	Type	Concentration
2	S16	[U-100% 13C; U-90% 15N]		1.5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
¹H	H2O	protons	4.732 ppm	internal	direct	0.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
¹H	H2O	protons	4.732 ppm	internal	direct	0.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
¹H	H2O	protons	4.732 ppm	internal	direct	0.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: -0.08 ppm, Outliers: 2 Detail

Release date

2000-12-06

Citation

Another piece of the Ribosome: Solution Structure of S16 and its Location in the 30S Subunit
Allard, P., Rak, A.V., Wimberly, B.T., Clemons, W.M., Kalinin, A., Helgstrand, M., Garber, M.B., Ramakrishnan, V., Hard, T.
Structure Fold. Des. (2000), 8, 875-882, DOI 10.1016/s0969-2126(00)00177-5

Related entities 1. S16, : 1 : 100 : 243 entities Detail

Interaction partners 1. S16, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 16 experiments Detail

1 DQF-COSY

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian INOVA (800 MHz)

Sample #1

Temperature 303 (±1) K

#	Name	Isotope labeling	Type	Concentration
1	S16			1.5 mM

Sample #2

Temperature 303 (±1) K

#	Name	Isotope labeling	Type	Concentration
2	S16	[U-100% 13C; U-90% 15N]		1.5 mM

2 TOCSY

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian INOVA (800 MHz)

Sample #1

Temperature 303 (±1) K

#	Name	Isotope labeling	Type	Concentration
1	S16			1.5 mM

Sample #2

Temperature 303 (±1) K

#	Name	Isotope labeling	Type	Concentration
2	S16	[U-100% 13C; U-90% 15N]		1.5 mM

3 NOESY

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian INOVA (800 MHz)

Sample #1

Temperature 303 (±1) K

#	Name	Isotope labeling	Type	Concentration
1	S16			1.5 mM

Sample #2

Temperature 303 (±1) K

#	Name	Isotope labeling	Type	Concentration
2	S16	[U-100% 13C; U-90% 15N]		1.5 mM

4 1H-15N HSQC

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian INOVA (800 MHz)

Sample #1

Temperature 303 (±1) K

#	Name	Isotope labeling	Type	Concentration
1	S16			1.5 mM

Sample #2

Temperature 303 (±1) K

#	Name	Isotope labeling	Type	Concentration
2	S16	[U-100% 13C; U-90% 15N]		1.5 mM

5 1H-15N TOCSY

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian INOVA (800 MHz)

Sample #1

Temperature 303 (±1) K

#	Name	Isotope labeling	Type	Concentration
1	S16			1.5 mM

Sample #2

Temperature 303 (±1) K

#	Name	Isotope labeling	Type	Concentration
2	S16	[U-100% 13C; U-90% 15N]		1.5 mM

6 1H-15N NOESY

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian INOVA (800 MHz)

Sample #1

Temperature 303 (±1) K

#	Name	Isotope labeling	Type	Concentration
1	S16			1.5 mM

Sample #2

Temperature 303 (±1) K

#	Name	Isotope labeling	Type	Concentration
2	S16	[U-100% 13C; U-90% 15N]		1.5 mM

7 HNHA

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian INOVA (800 MHz)

Sample #1

Temperature 303 (±1) K

#	Name	Isotope labeling	Type	Concentration
1	S16			1.5 mM

Sample #2

Temperature 303 (±1) K

#	Name	Isotope labeling	Type	Concentration
2	S16	[U-100% 13C; U-90% 15N]		1.5 mM

8 HNCA

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian INOVA (800 MHz)

Sample #1

Temperature 303 (±1) K

#	Name	Isotope labeling	Type	Concentration
1	S16			1.5 mM

Sample #2

Temperature 303 (±1) K

#	Name	Isotope labeling	Type	Concentration
2	S16	[U-100% 13C; U-90% 15N]		1.5 mM

9 HN(CO)CA

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian INOVA (800 MHz)

Sample #1

Temperature 303 (±1) K

#	Name	Isotope labeling	Type	Concentration
1	S16			1.5 mM

Sample #2

Temperature 303 (±1) K

#	Name	Isotope labeling	Type	Concentration
2	S16	[U-100% 13C; U-90% 15N]		1.5 mM

10 HNCACB

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian INOVA (800 MHz)

Sample #1

Temperature 303 (±1) K

#	Name	Isotope labeling	Type	Concentration
1	S16			1.5 mM

Sample #2

Temperature 303 (±1) K

#	Name	Isotope labeling	Type	Concentration
2	S16	[U-100% 13C; U-90% 15N]		1.5 mM

11 CBCA(CO)NH

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian INOVA (800 MHz)

Sample #1

Temperature 303 (±1) K

#	Name	Isotope labeling	Type	Concentration
1	S16			1.5 mM

Sample #2

Temperature 303 (±1) K

#	Name	Isotope labeling	Type	Concentration
2	S16	[U-100% 13C; U-90% 15N]		1.5 mM

12 H(CCO)NH

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian INOVA (800 MHz)

Sample #1

Temperature 303 (±1) K

#	Name	Isotope labeling	Type	Concentration
1	S16			1.5 mM

Sample #2

Temperature 303 (±1) K

#	Name	Isotope labeling	Type	Concentration
2	S16	[U-100% 13C; U-90% 15N]		1.5 mM

13 C(CO)NH

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian INOVA (800 MHz)

Sample #1

Temperature 303 (±1) K

#	Name	Isotope labeling	Type	Concentration
1	S16			1.5 mM

Sample #2

Temperature 303 (±1) K

#	Name	Isotope labeling	Type	Concentration
2	S16	[U-100% 13C; U-90% 15N]		1.5 mM

14 HCCH-TOCSY

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian INOVA (800 MHz)

Sample #1

Temperature 303 (±1) K

#	Name	Isotope labeling	Type	Concentration
1	S16			1.5 mM

Sample #2

Temperature 303 (±1) K

#	Name	Isotope labeling	Type	Concentration
2	S16	[U-100% 13C; U-90% 15N]		1.5 mM

15 (HB)CB(CGCD)HD

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian INOVA (800 MHz)

Sample #1

Temperature 303 (±1) K

#	Name	Isotope labeling	Type	Concentration
1	S16			1.5 mM

Sample #2

Temperature 303 (±1) K

#	Name	Isotope labeling	Type	Concentration
2	S16	[U-100% 13C; U-90% 15N]		1.5 mM

16 (HB)CB(CGCDCE)HE

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian INOVA (800 MHz)

Sample #1

Temperature 303 (±1) K

#	Name	Isotope labeling	Type	Concentration
1	S16			1.5 mM

Sample #2

Temperature 303 (±1) K

#	Name	Isotope labeling	Type	Concentration
2	S16	[U-100% 13C; U-90% 15N]		1.5 mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr4727_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr4727_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	shift_set_1	Warning	979		100.0 (chain: 1, length: 88)
1	Chemical shift references	chemical_shift_reference	OK	3		No information

Assigned chemical shifts

Saveframe: shift_set_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 88, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 627
  - ¹³C chemical shifts: 269
  - ¹⁵N chemical shifts: 83
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	567	537	94.7
¹³C chemical shifts	414	269	65.0
¹⁵N chemical shifts	104	83	79.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	178	172	96.6
¹³C chemical shifts	176	85	48.3
¹⁵N chemical shifts	84	77	91.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	389	365	93.8
¹³C chemical shifts	238	184	77.3
¹⁵N chemical shifts	20	6	30.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	46	46	100.0
¹³C chemical shifts	44	0	0.0
¹⁵N chemical shifts	2	2	100.0

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (1.267 seconds)

BMRB: 4727

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. S16, : 1 : 100 : 243 entities Detail

Interaction partners 1. S16, : 1 interactors Detail

Experiments performed 16 experiments Detail

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1