HMG-D complexed to a bulge DNA: an NMR study
MSDKPKRPLS AYMLWLNSAR ESIKRENPGI KVTEVAKRGG ELWRAMKDKS EWEAKAAKAK DDYDRAVKEF EANGGSSAAN GGGAKKRAKP AKKVAKKSKK EESDEDDDDE SE
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 53.8 % (692 of 1286) | 59.7 % (410 of 687) | 39.3 % (190 of 484) | 80.0 % (92 of 115) |
Backbone | 60.1 % (399 of 664) | 66.7 % (152 of 228) | 49.4 % (162 of 328) | 78.7 % (85 of 108) |
Sidechain | 49.2 % (357 of 726) | 56.2 % (258 of 459) | 35.4 % (92 of 260) | 100.0 % (7 of 7) |
Aromatic | 53.2 % (33 of 62) | 96.8 % (30 of 31) | 0.0 % (0 of 28) | 100.0 % (3 of 3) |
Methyl | 68.9 % (51 of 74) | 81.1 % (30 of 37) | 56.8 % (21 of 37) |
1. High Mobility Protein of Drosophila
MSDKPKRPLS AYMLWLNSAR ESIKRENPGI KVTEVAKRGG ELWRAMKDKS EWEAKAAKAK DDYDRAVKEF EANGGSSAAN GGGAKKRAKP AKKVAKKSKK EESDEDDDDE SETemperature 303 (±1) K, pH 6.0 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | High Mobility Protein of Drosophila | [U-95% 15N; U-95% 13C] | 0.8 ~ 1.0 mM |