BMRB: 4759

Backbone 1H, 15N and 13Calpha assigned chemical shifts for reduced Escherichia coli cytochrome b562

Authors

Assfalg, M., Banci, L., Bertini, I.

Assembly

cytochrome b562

Entity

1. cytochrome b562 (polymer, Thiol state: not present), 106 monomers, 11780.08 Da Detail

ADLEDNMETL NDNLKVIEKA DNAAQVKDAL TKMRAAALDA QKATPPKLED KSPDSPEMKD FRHGFDILVG QIDDALKLAN EGKVKEAQAA AEQLKTTRNA YHQKYR

2. HEM (non-polymer), 616.487 Da

Total weight

12396.567 Da

Max. entity weight

11780.08 Da

Source organism

Escherichia coli

Exptl. method

NMR

Refine. method

TORSION ANGLE DYNAMICS FOLLOWED BY ENERGY MINIMIZATION

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 87.7 %, Completeness: 24.5 %, Completeness (bb): 44.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	24.5 % (299 of 1218)	16.7 % (107 of 640)	22.2 % (103 of 464)	78.1 % (89 of 114)
Backbone	44.9 % (282 of 628)	44.1 % (93 of 211)	31.7 % (100 of 315)	87.3 % (89 of 102)
Sidechain	3.0 % (21 of 693)	3.3 % (14 of 429)	2.8 % (7 of 252)	0.0 % (0 of 12)
Aromatic	0.0 % (0 of 44)	0.0 % (0 of 22)	0.0 % (0 of 22)
Methyl	0.0 % (0 of 112)	0.0 % (0 of 56)	0.0 % (0 of 56)

1. cytochrome b562

ADLEDNMETL NDNLKVIEKA DNAAQVKDAL TKMRAAALDA QKATPPKLED KSPDSPEMKD FRHGFDILVG QIDDALKLAN EGKVKEAQAA AEQLKTTRNA YHQKYR

Show sample condition

Sample

Temperature 298 (±1) K, pH 4.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	cytochrome b562	[U-13C; U-15N]		2.0 mM

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Protein Blocks Logo

Calculated from 1 models in PDB: 1QPU, Strand ID: A Detail

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Release date

2000-07-20

Citation

Backbone 1H, 15N and 13Calpha assigned chemical shifts for reduced Escherichia coli cytochrome b56
Assfalg, M., Banci, L., Bertini, I.

Related entities 1. cytochrome b562, : 1 : 4 : 1 : 95 : 8 entities Detail

Interaction partners 1. cytochrome b562, : 16 interactors Detail

Experiments performed 6 experiments Detail